Involvement of Lipids in Dimethoate-Induced Inhibition of Testosterone Biosynthesis in Rat Interstitial Cells

The mechanism involved in the inhibition of testosterone (Te) biosynthesis after a sub-chronic exposure to low doses of dimethoate (D) was studied in rat interstitial cells (IC). Expression of COX-2 in IC isolated from D-treated rats increased by 44% over C data, while transcription of StAR decrease...

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Autores principales: Astiz, Mariana, Hurtado de Catalfo, Graciela E., Tacconi de Alaniz, María Josefa, Marra, Carlos Alberto
Formato: Articulo
Lenguaje:Inglés
Publicado: 2009
Materias:
Medicina
Arachidonic acid
COX-2
Dimethoate
Oxidative stress
Prostaglandins
Rat interstitial cells
StAR
h-CG
Rofecoxib
TROLOX
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/131025
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-131025
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Medicina
Arachidonic acid
COX-2
Dimethoate
Oxidative stress
Prostaglandins
Rat interstitial cells
StAR
h-CG
Rofecoxib
TROLOX
spellingShingle Medicina
Arachidonic acid
COX-2
Dimethoate
Oxidative stress
Prostaglandins
Rat interstitial cells
StAR
h-CG
Rofecoxib
TROLOX
Astiz, Mariana
Hurtado de Catalfo, Graciela E.
Tacconi de Alaniz, María Josefa
Marra, Carlos Alberto
Involvement of Lipids in Dimethoate-Induced Inhibition of Testosterone Biosynthesis in Rat Interstitial Cells
topic_facet Medicina
Arachidonic acid
COX-2
Dimethoate
Oxidative stress
Prostaglandins
Rat interstitial cells
StAR
h-CG
Rofecoxib
TROLOX
description The mechanism involved in the inhibition of testosterone (Te) biosynthesis after a sub-chronic exposure to low doses of dimethoate (D) was studied in rat interstitial cells (IC). Expression of COX-2 in IC isolated from D-treated rats increased by 44% over C data, while transcription of StAR decreased by approx. 50% and the expression of this protein was diminished by approximately 40%. PGE₂ and PGF<sub>2α</sub> were increased by 61 and 78%, respectively. Te concentration decreased by 49% in IC homogenates. Concomitantly, plasma concentration of LH and FSH both increased. Araquidonate (ARA) and C₂₂ fatty acyl chains in phospholipids from IC mitochondrial fraction decreased by approx. 30% after D treatment. Protein carbonyls, lipoperoxides and nitrite content increased while α-tocopherol and the antioxidant capacity of the soluble cellular fraction decreased significantly. Stimulation with h-CG 10 nM overnight failed to overcome the inhibition caused by D on both Te biosynthesis and 3β- and 17β-hydroxysteroid dehydrogenases. Decreased Te biosynthesis may be attributed to (1) inhibition of StAR protein activity due to the stimulation of COX-2 and the overproduction of PGF<sub>2α</sub>, (2) decreased stimulatory effect of ARA on StAR with a subsequent reduction in the availability of CHO for the androgenic pathway, and/or (3) indirect inhibition of steroidogenic enzymes by a lower transcriptional rate caused by elevated PGF<sub>2α</sub>. Rofecoxib administration prevents the deleterious effect(s) exerted by D.
format Articulo
Articulo
author Astiz, Mariana
Hurtado de Catalfo, Graciela E.
Tacconi de Alaniz, María Josefa
Marra, Carlos Alberto
author_facet Astiz, Mariana
Hurtado de Catalfo, Graciela E.
Tacconi de Alaniz, María Josefa
Marra, Carlos Alberto
author_sort Astiz, Mariana
title Involvement of Lipids in Dimethoate-Induced Inhibition of Testosterone Biosynthesis in Rat Interstitial Cells
title_short Involvement of Lipids in Dimethoate-Induced Inhibition of Testosterone Biosynthesis in Rat Interstitial Cells
title_full Involvement of Lipids in Dimethoate-Induced Inhibition of Testosterone Biosynthesis in Rat Interstitial Cells
title_fullStr Involvement of Lipids in Dimethoate-Induced Inhibition of Testosterone Biosynthesis in Rat Interstitial Cells
title_full_unstemmed Involvement of Lipids in Dimethoate-Induced Inhibition of Testosterone Biosynthesis in Rat Interstitial Cells
title_sort involvement of lipids in dimethoate-induced inhibition of testosterone biosynthesis in rat interstitial cells
publishDate 2009
url http://sedici.unlp.edu.ar/handle/10915/131025
work_keys_str_mv AT astizmariana involvementoflipidsindimethoateinducedinhibitionoftestosteronebiosynthesisinratinterstitialcells
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AT tacconidealanizmariajosefa involvementoflipidsindimethoateinducedinhibitionoftestosteronebiosynthesisinratinterstitialcells
AT marracarlosalberto involvementoflipidsindimethoateinducedinhibitionoftestosteronebiosynthesisinratinterstitialcells
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