Evaluation of Strontium-Containing PCL-PDIPF Scaffolds for Bone Tissue Engineering: <i>In Vitro</i> and <i>In Vivo Studies</i>
Bone tissue engineering (BTE) has the general objective of restoring and improving damaged bone. A very interesting strategy for BTE is to combine an adequate polymeric scaffold with an osteoinductive compound. Strontium is a divalent cation that can substitute calcium in hydroxyapatite and induce b...
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| Formato: | Articulo |
| Lenguaje: | Inglés |
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2019
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/130919 |
| Aporte de: |
| id |
I19-R120-10915-130919 |
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| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Ciencias Exactas Biología Bone marrow stromal cells RAW 264.7 macrophages Poly-ε-caprolactone Polydiisopropyl fumarate Strontium Bone regeneration |
| spellingShingle |
Ciencias Exactas Biología Bone marrow stromal cells RAW 264.7 macrophages Poly-ε-caprolactone Polydiisopropyl fumarate Strontium Bone regeneration Lino, Agustina Berenice McCarthy, Antonio Desmond Fernández, Juan Manuel Evaluation of Strontium-Containing PCL-PDIPF Scaffolds for Bone Tissue Engineering: <i>In Vitro</i> and <i>In Vivo Studies</i> |
| topic_facet |
Ciencias Exactas Biología Bone marrow stromal cells RAW 264.7 macrophages Poly-ε-caprolactone Polydiisopropyl fumarate Strontium Bone regeneration |
| description |
Bone tissue engineering (BTE) has the general objective of restoring and improving damaged bone. A very interesting strategy for BTE is to combine an adequate polymeric scaffold with an osteoinductive compound. Strontium is a divalent cation that can substitute calcium in hydroxyapatite and induce both anabolic and anti-catabolic effects in bone. On the other hand, systemic increases in Sr²⁺ levels can provoke adverse cardiovascular effects. In the present study we have developed a compatibilized blend of poly-ε-caprolactone (PCL) and polydiisopropyl fumarate (PDIPF) enriched with 1% or 5% Sr²⁺ and evaluated the applicability of these biomaterials for BTE, both <i>in vitro</i> and <i>in vivo</i>. <i>In vitro</i>, whereas Blend + 5% Sr²⁺ was pro-inflammatory and anti-osteogenic, Blend + 1% Sr²⁺ released very low quantities of the cation; was not cytotoxic for cultured macrophages; and showed improved osteocompatibility when used as a substratum for primary cultures of bone marrow stromal cells. <i>In vivo</i>, implants with Blend + 1% Sr²⁺ significantly increased bone tissue regeneration and improved fibrous bridging (vs. Blend alone), while neither inducing a local inflammatory response nor increased serum levels of Sr²⁺. These results indicate that our compatibilized blend of PCL-PDIPF enriched with 1% Sr²⁺ could be useful for BTE. |
| format |
Articulo Articulo |
| author |
Lino, Agustina Berenice McCarthy, Antonio Desmond Fernández, Juan Manuel |
| author_facet |
Lino, Agustina Berenice McCarthy, Antonio Desmond Fernández, Juan Manuel |
| author_sort |
Lino, Agustina Berenice |
| title |
Evaluation of Strontium-Containing PCL-PDIPF Scaffolds for Bone Tissue Engineering: <i>In Vitro</i> and <i>In Vivo Studies</i> |
| title_short |
Evaluation of Strontium-Containing PCL-PDIPF Scaffolds for Bone Tissue Engineering: <i>In Vitro</i> and <i>In Vivo Studies</i> |
| title_full |
Evaluation of Strontium-Containing PCL-PDIPF Scaffolds for Bone Tissue Engineering: <i>In Vitro</i> and <i>In Vivo Studies</i> |
| title_fullStr |
Evaluation of Strontium-Containing PCL-PDIPF Scaffolds for Bone Tissue Engineering: <i>In Vitro</i> and <i>In Vivo Studies</i> |
| title_full_unstemmed |
Evaluation of Strontium-Containing PCL-PDIPF Scaffolds for Bone Tissue Engineering: <i>In Vitro</i> and <i>In Vivo Studies</i> |
| title_sort |
evaluation of strontium-containing pcl-pdipf scaffolds for bone tissue engineering: <i>in vitro</i> and <i>in vivo studies</i> |
| publishDate |
2019 |
| url |
http://sedici.unlp.edu.ar/handle/10915/130919 |
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Repositorios |
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