ISSR markers to explore entomopathogenic fungi genetic diversity: Implications for biological control of tobacco pests

Tobacco is one of the major industrial crops cultivated worldwide. Chemical control is the main method employed to reduce damage by insect pests. The use of entomopathogenic fungi represents an alternative to replace insecticides. The search for effective strains in the field constitutes a first ste...

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Detalles Bibliográficos
Autores principales: Vianna, María Florencia, Pelizza, Sebastián Alberto, Russo, María Leticia, Toledo, Andrea Vanesa, Mourelos, Cecilia Alejandra, Scorsetti, Ana Clara
Formato: Articulo
Lenguaje:Inglés
Publicado: 2020
Materias:
Ciencias Naturales
Ciencias Agrarias
Beauveria bassiana
Diabrotica speciose
genetic polymorphism
Helicoverpa gelotopoeon
pathogenicity
Purpureocillium lilacinum
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/130867
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-130867
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Naturales
Ciencias Agrarias
Beauveria bassiana
Diabrotica speciose
genetic polymorphism
Helicoverpa gelotopoeon
pathogenicity
Purpureocillium lilacinum
spellingShingle Ciencias Naturales
Ciencias Agrarias
Beauveria bassiana
Diabrotica speciose
genetic polymorphism
Helicoverpa gelotopoeon
pathogenicity
Purpureocillium lilacinum
Vianna, María Florencia
Pelizza, Sebastián Alberto
Russo, María Leticia
Toledo, Andrea Vanesa
Mourelos, Cecilia Alejandra
Scorsetti, Ana Clara
ISSR markers to explore entomopathogenic fungi genetic diversity: Implications for biological control of tobacco pests
topic_facet Ciencias Naturales
Ciencias Agrarias
Beauveria bassiana
Diabrotica speciose
genetic polymorphism
Helicoverpa gelotopoeon
pathogenicity
Purpureocillium lilacinum
description Tobacco is one of the major industrial crops cultivated worldwide. Chemical control is the main method employed to reduce damage by insect pests. The use of entomopathogenic fungi represents an alternative to replace insecticides. The search for effective strains in the field constitutes a first step when developing a formulation. The objective of this work was to study genetic differences among isolates of entomopathogenic fungi obtained from tobacco grown soils using ISSR markers. The pathogenicity of the strains towards <i>Helicoverpa gelotopoeon</i> and <i>Diabrotica speciosa</i> was also assessed in order to search for a relationship between virulence and genetic diversity. Nineteen isolates were identified according to morphological features and molecular techniques as <i>Beauveria bassiana</i> (11) and <i>Purpureocillium lilacinum</i> (8). The diversity tree generated by ISSR analysis showed a high diversity among the strains. The pathogenicity towards <i>H. gelotopoeon</i> and <i>D. speciosa</i> was assessed and the logistic models generated showed that <i>B. bassiana</i> isolates LPSc1215 and LPSc1364 were the most pathogenic against both insect pests tested. In the diversity tree, these strains were grouped in a same cluster with a similarity level of approximately 85%, indicating a possible relationship between virulence and the band pattern generated.
format Articulo
Articulo
author Vianna, María Florencia
Pelizza, Sebastián Alberto
Russo, María Leticia
Toledo, Andrea Vanesa
Mourelos, Cecilia Alejandra
Scorsetti, Ana Clara
author_facet Vianna, María Florencia
Pelizza, Sebastián Alberto
Russo, María Leticia
Toledo, Andrea Vanesa
Mourelos, Cecilia Alejandra
Scorsetti, Ana Clara
author_sort Vianna, María Florencia
title ISSR markers to explore entomopathogenic fungi genetic diversity: Implications for biological control of tobacco pests
title_short ISSR markers to explore entomopathogenic fungi genetic diversity: Implications for biological control of tobacco pests
title_full ISSR markers to explore entomopathogenic fungi genetic diversity: Implications for biological control of tobacco pests
title_fullStr ISSR markers to explore entomopathogenic fungi genetic diversity: Implications for biological control of tobacco pests
title_full_unstemmed ISSR markers to explore entomopathogenic fungi genetic diversity: Implications for biological control of tobacco pests
title_sort issr markers to explore entomopathogenic fungi genetic diversity: implications for biological control of tobacco pests
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/130867
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