Ligninolytic Ability and Potential Biotechnology Applications of the South American Fungus Pleurotus laciniatocrenatus

The extracellular ligninolytic enzyme system of Pleurotus laciniatocrenatus, grown under different culture conditions, was characterized and the ability of this strain to degrade different components of Eucalyptus globulus wood was determined. In shaken liquid c...

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Detalles Bibliográficos
Autores principales:	Saparrat, Mario Carlos Nazareno, Guillén, Francisco
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2005
Materias:	Ciencias Naturales Vanillic Acid Ligninolytic Enzyme Versatile Peroxidase Ligninolytic Fungus Pleurotus Species
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/130851
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-130851
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Naturales Vanillic Acid Ligninolytic Enzyme Versatile Peroxidase Ligninolytic Fungus Pleurotus Species
spellingShingle	Ciencias Naturales Vanillic Acid Ligninolytic Enzyme Versatile Peroxidase Ligninolytic Fungus Pleurotus Species Saparrat, Mario Carlos Nazareno Guillén, Francisco Ligninolytic Ability and Potential Biotechnology Applications of the South American Fungus <i>Pleurotus laciniatocrenatus</i>
topic_facet	Ciencias Naturales Vanillic Acid Ligninolytic Enzyme Versatile Peroxidase Ligninolytic Fungus Pleurotus Species
description	The extracellular ligninolytic enzyme system of <i>Pleurotus laciniatocrenatus</i>, grown under different culture conditions, was characterized and the ability of this strain to degrade different components of <i>Eucalyptus globulus</i> wood was determined. In shaken liquid cultures grown on a C-limited medium supplemented with yeast extract (0.1 %) and peptone (0.5 %), the fungus produced extracellular aryl-alcohol oxidase (Aao), laccase (Lac), manganese-dependent peroxidase (MnP) and manganese-independent peroxidase (MiP) activities, their maximum levels being, respectively, about 600, 50, 1360, and 920 pkat/mL. The supplementation of 1 mmol/L vanillic acid and 150 µmol/L CuSO4 produced an increase of Lac activity levels up to 4-fold and 68.3-fold, respectively. No significant differences were found in the levels of the other ligninolytic enzyme activities when compared to the basal medium. Solid-state fermentation cultures on <i>E. globulus</i> wood chips revealed Lac and MiP activities. These cultures showed degradative activity on lignin and lipophilic wood extractives.
format	Articulo Articulo
author	Saparrat, Mario Carlos Nazareno Guillén, Francisco
author_facet	Saparrat, Mario Carlos Nazareno Guillén, Francisco
author_sort	Saparrat, Mario Carlos Nazareno
title	Ligninolytic Ability and Potential Biotechnology Applications of the South American Fungus <i>Pleurotus laciniatocrenatus</i>
title_short	Ligninolytic Ability and Potential Biotechnology Applications of the South American Fungus <i>Pleurotus laciniatocrenatus</i>
title_full	Ligninolytic Ability and Potential Biotechnology Applications of the South American Fungus <i>Pleurotus laciniatocrenatus</i>
title_fullStr	Ligninolytic Ability and Potential Biotechnology Applications of the South American Fungus <i>Pleurotus laciniatocrenatus</i>
title_full_unstemmed	Ligninolytic Ability and Potential Biotechnology Applications of the South American Fungus <i>Pleurotus laciniatocrenatus</i>
title_sort	ligninolytic ability and potential biotechnology applications of the south american fungus <i>pleurotus laciniatocrenatus</i>
publishDate	2005
url	http://sedici.unlp.edu.ar/handle/10915/130851
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bdutipo_str	Repositorios
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Ligninolytic Ability and Potential Biotechnology Applications of the South American Fungus <i>Pleurotus laciniatocrenatus</i>

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