Response of the fungus Pseudocercospora griseola f. mesoamericana to Tricyclazole

Pseudocercospora griseola, an anamorph of Mycosphaerella, causes Angular Leaf Spot (ALS). The mycelia and conidia from P. griseola are coloured due to the synthesis of 1,8 dihydroxynaphthalene (DHN)-melanin. The aim of this work was to identif...

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Detalles Bibliográficos
Autores principales:	Bárcena, Alejandra, Petroselli, Gabriela, Velasquez, Silvia M., Estevez, José M., Erra-Balsells, Rosa, Balatti, Pedro Alberto, Saparrat, Mario Carlos Nazareno
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2015
Materias:	Ciencias Naturales Pseudocercospora griseola f. mesoamericana Phaseolus vulgaris Melanin Tricyclazole UV-MALDI-TOFMS Microscopy FTIR
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/130754
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-130754
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Naturales Pseudocercospora griseola f. mesoamericana Phaseolus vulgaris Melanin Tricyclazole UV-MALDI-TOFMS Microscopy FTIR
spellingShingle	Ciencias Naturales Pseudocercospora griseola f. mesoamericana Phaseolus vulgaris Melanin Tricyclazole UV-MALDI-TOFMS Microscopy FTIR Bárcena, Alejandra Petroselli, Gabriela Velasquez, Silvia M. Estevez, José M. Erra-Balsells, Rosa Balatti, Pedro Alberto Saparrat, Mario Carlos Nazareno Response of the fungus <i>Pseudocercospora griseola f. mesoamericana</i> to Tricyclazole
topic_facet	Ciencias Naturales Pseudocercospora griseola f. mesoamericana Phaseolus vulgaris Melanin Tricyclazole UV-MALDI-TOFMS Microscopy FTIR
description	<i>Pseudocercospora griseola</i>, an anamorph of <i>Mycosphaerella</i>, causes Angular Leaf Spot (ALS). The mycelia and conidia from <i>P. griseola</i> are coloured due to the synthesis of 1,8 dihydroxynaphthalene (DHN)-melanin. The aim of this work was to identify in <i>P. griseola f. mesoamericana</i> isolate T4, a highly pigmented fungus, intermediary compounds as a result of the inhibition of melanin synthesis by tricyclazole, and to analyze at the structural level the localization of these dark pigments. The main metabolites were analyzed using ultraviolet matrix-assisted laser desorption-ionization mass spectrometry (UV–MALDI MS). Tricyclazole affected <i>P. griseola f. mesoamericana</i> in several different ways. The most evident effect was the reduction of melanin synthesis, and therefore diffusible shunt products were found and identified. Flaviolin was the main intermediate metabolite found in cultures supplemented with tricyclazole. This inhibitor, which affected pigmentation and the cell wall structure of mycelium, revealed macroscopically by the reduction in growth, decreased the stratification and deposition of melanin in the hyphal wall. These results suggest a possible role of tricyclazole to control ALS.
format	Articulo Articulo
author	Bárcena, Alejandra Petroselli, Gabriela Velasquez, Silvia M. Estevez, José M. Erra-Balsells, Rosa Balatti, Pedro Alberto Saparrat, Mario Carlos Nazareno
author_facet	Bárcena, Alejandra Petroselli, Gabriela Velasquez, Silvia M. Estevez, José M. Erra-Balsells, Rosa Balatti, Pedro Alberto Saparrat, Mario Carlos Nazareno
author_sort	Bárcena, Alejandra
title	Response of the fungus <i>Pseudocercospora griseola f. mesoamericana</i> to Tricyclazole
title_short	Response of the fungus <i>Pseudocercospora griseola f. mesoamericana</i> to Tricyclazole
title_full	Response of the fungus <i>Pseudocercospora griseola f. mesoamericana</i> to Tricyclazole
title_fullStr	Response of the fungus <i>Pseudocercospora griseola f. mesoamericana</i> to Tricyclazole
title_full_unstemmed	Response of the fungus <i>Pseudocercospora griseola f. mesoamericana</i> to Tricyclazole
title_sort	response of the fungus <i>pseudocercospora griseola f. mesoamericana</i> to tricyclazole
publishDate	2015
url	http://sedici.unlp.edu.ar/handle/10915/130754
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Response of the fungus <i>Pseudocercospora griseola f. mesoamericana</i> to Tricyclazole

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