MUC1 oncogene amplification correlates with protein overexpression in invasive breast carcinoma cells

The MUC1 gene is aberrantly overexpressed in approximately 90% of human breast cancers. Several studies have shown that MUC1 overexpression is due to transcriptional regulatory events. However, the importance of gene amplification as a mechanism leading to the increase of MUC1 expression in breast c...

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Autores principales: Lacunza, Ezequiel, Baudis, Michael, Colussi, Andrea G., Segal-Eiras, Amada, Croce, María Virginia, Abba, Martín Carlos
Formato: Articulo
Lenguaje:Inglés
Publicado: 2010
Materias:
Medicina
MUC1 gene
breast cancer
gene amplification
protein expression
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/128899
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-128899
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Medicina
MUC1 gene
breast cancer
gene amplification
protein expression
spellingShingle Medicina
MUC1 gene
breast cancer
gene amplification
protein expression
Lacunza, Ezequiel
Baudis, Michael
Colussi, Andrea G.
Segal-Eiras, Amada
Croce, María Virginia
Abba, Martín Carlos
MUC1 oncogene amplification correlates with protein overexpression in invasive breast carcinoma cells
topic_facet Medicina
MUC1 gene
breast cancer
gene amplification
protein expression
description The MUC1 gene is aberrantly overexpressed in approximately 90% of human breast cancers. Several studies have shown that MUC1 overexpression is due to transcriptional regulatory events. However, the importance of gene amplification as a mechanism leading to the increase of MUC1 expression in breast cancer has been poorly characterized. The aim of this study was to evaluate the role of MUC1 gene amplification and protein expression in human breast cancer development. By means of real-time quantitative polymerase chain reaction and immunohistochemical methods, 83 breast tissue samples were analyzed for MUC1 gene amplification and protein expression. This analysis showed MUC1 genomic amplification and a positive association with the histopathological group in 12% (1 out of 8) of benign lesions and 38% (23 out of 60) of primary invasive breast carcinoma samples (P = 0.004). Array-comparative genomic hybridization meta-analysis of 886 primary invasive breast carcinomas obtained from 22 studies showed MUC1 genomic gain in 43.7% (387 out of 886) of the samples. Moreover, we identified a highly statistical significant association between MUC1 gene amplification and MUC1 protein expression assessed by immunohistochemistry and Western blot test (P < 0.0001). In conclusion, this study demonstrated that MUC1 copy number increases from normal breast tissue to primary invasive breast carcinomas in correlation with MUC1 protein expression.
format Articulo
Articulo
author Lacunza, Ezequiel
Baudis, Michael
Colussi, Andrea G.
Segal-Eiras, Amada
Croce, María Virginia
Abba, Martín Carlos
author_facet Lacunza, Ezequiel
Baudis, Michael
Colussi, Andrea G.
Segal-Eiras, Amada
Croce, María Virginia
Abba, Martín Carlos
author_sort Lacunza, Ezequiel
title MUC1 oncogene amplification correlates with protein overexpression in invasive breast carcinoma cells
title_short MUC1 oncogene amplification correlates with protein overexpression in invasive breast carcinoma cells
title_full MUC1 oncogene amplification correlates with protein overexpression in invasive breast carcinoma cells
title_fullStr MUC1 oncogene amplification correlates with protein overexpression in invasive breast carcinoma cells
title_full_unstemmed MUC1 oncogene amplification correlates with protein overexpression in invasive breast carcinoma cells
title_sort muc1 oncogene amplification correlates with protein overexpression in invasive breast carcinoma cells
publishDate 2010
url http://sedici.unlp.edu.ar/handle/10915/128899
work_keys_str_mv AT lacunzaezequiel muc1oncogeneamplificationcorrelateswithproteinoverexpressionininvasivebreastcarcinomacells
AT baudismichael muc1oncogeneamplificationcorrelateswithproteinoverexpressionininvasivebreastcarcinomacells
AT colussiandreag muc1oncogeneamplificationcorrelateswithproteinoverexpressionininvasivebreastcarcinomacells
AT segaleirasamada muc1oncogeneamplificationcorrelateswithproteinoverexpressionininvasivebreastcarcinomacells
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bdutipo_str Repositorios
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