Impact of brassinosteroids and ethylene on ascorbic acid accumulation in tomato leaves

Plant steroid hormones brassinosteroids (BRs) and the gaseous hormone ethylene (ET) alter the ascorbic acid–glutathione (AA–GSH) levels in tomato (Solanum lycopersicum L.) plants. The interaction of these hormones in regulating antioxidant metabolism is however unknown. The combined use of genetics...

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Autores principales: Mazorra, Luis Miguel, Senn, María Eugenia, Gergoff Grozeff, Gustavo Esteban, Fanello, Diego Darío, Carrión, Cristian Antonio, Núñez, Miriam, Bishop, Gerard J., Bartoli, Carlos Guillermo
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2014
Materias:
Botánica
Antioxidants
Ascorbic acid
Brassinosteroids
Ethylene
Leaves
RespirationTomato
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/128719
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-128719
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Botánica
Antioxidants
Ascorbic acid
Brassinosteroids
Ethylene
Leaves
RespirationTomato
spellingShingle Botánica
Antioxidants
Ascorbic acid
Brassinosteroids
Ethylene
Leaves
RespirationTomato
Mazorra, Luis Miguel
Senn, María Eugenia
Gergoff Grozeff, Gustavo Esteban
Fanello, Diego Darío
Carrión, Cristian Antonio
Núñez, Miriam
Bishop, Gerard J.
Bartoli, Carlos Guillermo
Impact of brassinosteroids and ethylene on ascorbic acid accumulation in tomato leaves
topic_facet Botánica
Antioxidants
Ascorbic acid
Brassinosteroids
Ethylene
Leaves
RespirationTomato
description Plant steroid hormones brassinosteroids (BRs) and the gaseous hormone ethylene (ET) alter the ascorbic acid–glutathione (AA–GSH) levels in tomato (Solanum lycopersicum L.) plants. The interaction of these hormones in regulating antioxidant metabolism is however unknown. The combined use of genetics (BR-mutants) and chemical application (BR/ET-related chemicals) shows that BRs and ET signalling pathways interact, to regulate leaf AA content and synthesis. BR-deficient (dx) leaves display low total AA but BR-accumulating (35S:D) leaves show normal total AA content. Leaves with either BR levels lower or higher than wild type plants showed a higher oxidised AA redox state. The activity of l-galactono-1,4-lactone dehydrogenase (l-GalLDH), the mitochondrial enzyme that catalyses the last step in AA synthesis is lower in dx and higher in 35S:D plants. BR-deficient mutants show higher ET production but it is restored to normal levels when BR content is increased in 35S:D plants. Suppression of ET signalling using 1-methylcyclopropene in dx and 35S:D plants restored leaf AA content and l-GalLDH activity, to the values observed in wild type. The suppression of ET action in dx and 35S:D leaves leads to the respective decreasing and increasing respiration, indicating an opposite response compared to AA synthesis. This inverse relationship is lacking in ET suppressed dx plants in response to external BRs. The modifications in the in vivo activity of l-GalLDH activity do not correlate with changes in the level of the enzyme. Taken together, these data suggest that ET suppresses and BRs promote AA synthesis and accumulation.
format Articulo
Preprint
author Mazorra, Luis Miguel
Senn, María Eugenia
Gergoff Grozeff, Gustavo Esteban
Fanello, Diego Darío
Carrión, Cristian Antonio
Núñez, Miriam
Bishop, Gerard J.
Bartoli, Carlos Guillermo
author_facet Mazorra, Luis Miguel
Senn, María Eugenia
Gergoff Grozeff, Gustavo Esteban
Fanello, Diego Darío
Carrión, Cristian Antonio
Núñez, Miriam
Bishop, Gerard J.
Bartoli, Carlos Guillermo
author_sort Mazorra, Luis Miguel
title Impact of brassinosteroids and ethylene on ascorbic acid accumulation in tomato leaves
title_short Impact of brassinosteroids and ethylene on ascorbic acid accumulation in tomato leaves
title_full Impact of brassinosteroids and ethylene on ascorbic acid accumulation in tomato leaves
title_fullStr Impact of brassinosteroids and ethylene on ascorbic acid accumulation in tomato leaves
title_full_unstemmed Impact of brassinosteroids and ethylene on ascorbic acid accumulation in tomato leaves
title_sort impact of brassinosteroids and ethylene on ascorbic acid accumulation in tomato leaves
publishDate 2014
url http://sedici.unlp.edu.ar/handle/10915/128719
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