Determination of thermodynamic binding constants by affinity capillary electrophoresis

A strategy to study thermodynamic binding constants by affinity capillary electrophoresis (ACE) is presented. In order to simplify mathematical treatment, analogy with acid-base dissociation equilibrium is proposed: instead of ligand concentration [X], negative logarithm of ligand concentration (or...

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Detalles Bibliográficos
Autores principales:	Lancioni, Carlina, Keunchkarian, Sonia, Castells, Cecilia Beatriz Marta, Gagliardi, Leonardo Gabriel
Formato:	Articulo Preprint
Lenguaje:	Inglés
Publicado:	2019
Materias:	Química Thermodynamic binding constant Affinity capillary electrophoresis Chiral separation Cyclodextrin
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/128708
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-128708
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Química Thermodynamic binding constant Affinity capillary electrophoresis Chiral separation Cyclodextrin
spellingShingle	Química Thermodynamic binding constant Affinity capillary electrophoresis Chiral separation Cyclodextrin Lancioni, Carlina Keunchkarian, Sonia Castells, Cecilia Beatriz Marta Gagliardi, Leonardo Gabriel Determination of thermodynamic binding constants by affinity capillary electrophoresis
topic_facet	Química Thermodynamic binding constant Affinity capillary electrophoresis Chiral separation Cyclodextrin
description	A strategy to study thermodynamic binding constants by affinity capillary electrophoresis (ACE) is presented. In order to simplify mathematical treatment, analogy with acid-base dissociation equilibrium is proposed: instead of ligand concentration [X], negative logarithm of ligand concentration (or activity), pX = -log[X], is used. On this base, and taking into account ionic activities, a general procedure for obtaining thermodynamic binding constants is proposed. In addition, the method provides electrophoretic mobilities of the free analyte and analyte-ligand complex, even when binding constants are low and thus, the complexed analyte fraction is also low. This is useful as a base to rationally analyze a diversity of situations, i.e., different mathematical dependencies are obtained when analytes and ligands with different charges are combined. Practical considerations are given for carrying out a full experimental design. Enantiomeric ACE separation based on the use of chiral selectors is addressed. 2-hydroxypropyl-β-cyclodextrin was chosen as a model ligand, and both enantiomeric forms of four pharmaceutical drugs (propranolol, pindolol, oxprenolol and homatropine methylbromide) were considered as model analytes. Practical aspects are detailed and thermodynamic binding constants as well as free and complexed analytes mobilities are determined.
format	Articulo Preprint
author	Lancioni, Carlina Keunchkarian, Sonia Castells, Cecilia Beatriz Marta Gagliardi, Leonardo Gabriel
author_facet	Lancioni, Carlina Keunchkarian, Sonia Castells, Cecilia Beatriz Marta Gagliardi, Leonardo Gabriel
author_sort	Lancioni, Carlina
title	Determination of thermodynamic binding constants by affinity capillary electrophoresis
title_short	Determination of thermodynamic binding constants by affinity capillary electrophoresis
title_full	Determination of thermodynamic binding constants by affinity capillary electrophoresis
title_fullStr	Determination of thermodynamic binding constants by affinity capillary electrophoresis
title_full_unstemmed	Determination of thermodynamic binding constants by affinity capillary electrophoresis
title_sort	determination of thermodynamic binding constants by affinity capillary electrophoresis
publishDate	2019
url	http://sedici.unlp.edu.ar/handle/10915/128708
work_keys_str_mv	AT lancionicarlina determinationofthermodynamicbindingconstantsbyaffinitycapillaryelectrophoresis AT keunchkariansonia determinationofthermodynamicbindingconstantsbyaffinitycapillaryelectrophoresis AT castellsceciliabeatrizmarta determinationofthermodynamicbindingconstantsbyaffinitycapillaryelectrophoresis AT gagliardileonardogabriel determinationofthermodynamicbindingconstantsbyaffinitycapillaryelectrophoresis
bdutipo_str	Repositorios
_version_	1764820452761403393

Determination of thermodynamic binding constants by affinity capillary electrophoresis

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