Diacylglycerol generated in CHO cell plasma membrane by phospholipase C is used for triacylglycerol synthesis

The diacylglycerol (DAG) signal generated from membrane phospholipids by hormone-activated phospholipases is attenuated by mechanisms that include lipolysis or phospholipid resynthesis. To determine whether the DAG signal might also be terminated by incorporation of DAG into triacylglycerol (TAG), w...

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Autores principales: Igal, Rubén Ariel, Caviglia, Jorge Matías, Tacconi de Gómez Dumm, Irma Nelva, Coleman, Rosalind A.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2001
Materias:
Medicina
diacylglycerol lipase inhibitor
diacylglycerol kinase inhibitor
phospholipids
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/128705
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-128705
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Medicina
diacylglycerol lipase inhibitor
diacylglycerol kinase inhibitor
phospholipids
spellingShingle Medicina
diacylglycerol lipase inhibitor
diacylglycerol kinase inhibitor
phospholipids
Igal, Rubén Ariel
Caviglia, Jorge Matías
Tacconi de Gómez Dumm, Irma Nelva
Coleman, Rosalind A.
Diacylglycerol generated in CHO cell plasma membrane by phospholipase C is used for triacylglycerol synthesis
topic_facet Medicina
diacylglycerol lipase inhibitor
diacylglycerol kinase inhibitor
phospholipids
description The diacylglycerol (DAG) signal generated from membrane phospholipids by hormone-activated phospholipases is attenuated by mechanisms that include lipolysis or phospholipid resynthesis. To determine whether the DAG signal might also be terminated by incorporation of DAG into triacylglycerol (TAG), we studied the direct formation of TAG from endogenous DAG generated by bacterial phospholipase C (PLC). When Chinese hamster ovary (CHO) cells prelabeled with [¹⁴C]oleate were treated with PLC from <i>Clostridium perfringens</i> for 6 h, [¹⁴C]phospholipid decreased 15% and labeled TAG increased 60%. This transfer of ¹⁴C label was even greater when the cells were simultaneously exposed to PLC and 100 μM oleic acid. PLC as well as oleate treatment concomitantly increased the TAG mass within the cell. Moreover, when phospholipids were prelabeled with [³H]glycerol, a subsequent increase in [³H]TAG indicated that an intact DAG moiety was channeled into the TAG structure. Incubating CHO cells with the diacylglycerol kinase inhibitor R59022 enhanced the formation of TAG from phospholipids hydrolyzed by PLC or by PLC in the presence of 100 μM oleate, but not by incubation with oleate alone, indicating that the DAG released from plasma membrane phospholipids does not require the formation of a phosphatidic acid precursor for TAG synthesis. Similarly, the diacylglycerol lipase inhibitor RHC 80267 did not alter TAG synthesis from plasma membrane DAG, further supporting direct incorporation of DAG into TAG. These studies indicate that DAG derived from plasma membrane phospholipid is largely used for TAG formation, and support the view that this mechanism can terminate DAG signals. The studies also suggest that a transport mechanism exists to move plasma membrane-derived DAG to the endoplasmic reticulum.
format Articulo
Articulo
author Igal, Rubén Ariel
Caviglia, Jorge Matías
Tacconi de Gómez Dumm, Irma Nelva
Coleman, Rosalind A.
author_facet Igal, Rubén Ariel
Caviglia, Jorge Matías
Tacconi de Gómez Dumm, Irma Nelva
Coleman, Rosalind A.
author_sort Igal, Rubén Ariel
title Diacylglycerol generated in CHO cell plasma membrane by phospholipase C is used for triacylglycerol synthesis
title_short Diacylglycerol generated in CHO cell plasma membrane by phospholipase C is used for triacylglycerol synthesis
title_full Diacylglycerol generated in CHO cell plasma membrane by phospholipase C is used for triacylglycerol synthesis
title_fullStr Diacylglycerol generated in CHO cell plasma membrane by phospholipase C is used for triacylglycerol synthesis
title_full_unstemmed Diacylglycerol generated in CHO cell plasma membrane by phospholipase C is used for triacylglycerol synthesis
title_sort diacylglycerol generated in cho cell plasma membrane by phospholipase c is used for triacylglycerol synthesis
publishDate 2001
url http://sedici.unlp.edu.ar/handle/10915/128705
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AT cavigliajorgematias diacylglycerolgeneratedinchocellplasmamembranebyphospholipasecisusedfortriacylglycerolsynthesis
AT tacconidegomezdummirmanelva diacylglycerolgeneratedinchocellplasmamembranebyphospholipasecisusedfortriacylglycerolsynthesis
AT colemanrosalinda diacylglycerolgeneratedinchocellplasmamembranebyphospholipasecisusedfortriacylglycerolsynthesis
bdutipo_str Repositorios
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