Decrease in PTEN and increase in Akt expression and neuron size in aged rat spinal cord

PTEN is a tumor suppressor gene known to play an important role in the regulation of cell size. In this study we compared PTEN expression in the spinal cord of young (5 months old) vs. aged (32 months old) female rats and correlated them with alterations in neuron size and morphology in the same ani...

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Autores principales: Rodrigues De Amorim, Miguel Augusto, García Segura, Luis M., Goya, Rodolfo Gustavo, Portiansky, Enrique Leo
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2010
Materias:
Ciencias Médicas
Aging
Morphometry
Motoneurons
Neuronal size
Protein phosphatase
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/128558
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-128558
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Aging
Morphometry
Motoneurons
Neuronal size
Protein phosphatase
spellingShingle Ciencias Médicas
Aging
Morphometry
Motoneurons
Neuronal size
Protein phosphatase
Rodrigues De Amorim, Miguel Augusto
García Segura, Luis M.
Goya, Rodolfo Gustavo
Portiansky, Enrique Leo
Decrease in PTEN and increase in Akt expression and neuron size in aged rat spinal cord
topic_facet Ciencias Médicas
Aging
Morphometry
Motoneurons
Neuronal size
Protein phosphatase
description PTEN is a tumor suppressor gene known to play an important role in the regulation of cell size. In this study we compared PTEN expression in the spinal cord of young (5 months old) vs. aged (32 months old) female rats and correlated them with alterations in neuron size and morphology in the same animals. Total and phosphorylated PTEN (pPTEN) as well as its downstream target phosphorylated Akt (pAkt) were assessed by Western blotting. Spinal cord neurons were morphometrically characterized. Total PTEN, pPTEN and total Akt expression were significantly higher in young rats than in aged animals. Expression of pAkt was stronger in aged animals. A significant increase in neuronal size was observed in large motoneurons of aged as compared with young rats. Our data show that in the spinal cord of rats, neuronal PTEN expression diminishes with advanced age while neuronal size increases. These results suggest that in the spinal cord, an age-related reduction in PTEN and increase of pAkt expression may be involved in the progressive enlargement of neurons.
format Articulo
Preprint
author Rodrigues De Amorim, Miguel Augusto
García Segura, Luis M.
Goya, Rodolfo Gustavo
Portiansky, Enrique Leo
author_facet Rodrigues De Amorim, Miguel Augusto
García Segura, Luis M.
Goya, Rodolfo Gustavo
Portiansky, Enrique Leo
author_sort Rodrigues De Amorim, Miguel Augusto
title Decrease in PTEN and increase in Akt expression and neuron size in aged rat spinal cord
title_short Decrease in PTEN and increase in Akt expression and neuron size in aged rat spinal cord
title_full Decrease in PTEN and increase in Akt expression and neuron size in aged rat spinal cord
title_fullStr Decrease in PTEN and increase in Akt expression and neuron size in aged rat spinal cord
title_full_unstemmed Decrease in PTEN and increase in Akt expression and neuron size in aged rat spinal cord
title_sort decrease in pten and increase in akt expression and neuron size in aged rat spinal cord
publishDate 2010
url http://sedici.unlp.edu.ar/handle/10915/128558
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