Changes in global gene expression during in vitro decidualization of rat endometrial stromal cells

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During the preimplantation phase of pregnancy the endometrial stroma differentiates into decidua, a process that implies numerous morphological changes and is an example of physiological transdifferentiation. Here we show that UIII rat endometrial stromal cells cultured in the presence of calf serum...

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Autores principales: Vallejo, Griselda, Maschi, Darío, Mestre Citrinovitz, Ana Cecilia, Aiba, Kazuhiro, Maronna, Ricardo Antonio, Yohai, Victor, Ko, Minoru S.H., Beato, Miguel, Saragüeta, Patricia
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2010
Materias:
Biología
Gene expression
Microarrays
Decidualization
UIII cells
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/128408
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-128408
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Gene expression
Microarrays
Decidualization
UIII cells
spellingShingle Biología
Gene expression
Microarrays
Decidualization
UIII cells
Vallejo, Griselda
Maschi, Darío
Mestre Citrinovitz, Ana Cecilia
Aiba, Kazuhiro
Maronna, Ricardo Antonio
Yohai, Victor
Ko, Minoru S.H.
Beato, Miguel
Saragüeta, Patricia
Changes in global gene expression during in vitro decidualization of rat endometrial stromal cells
topic_facet Biología
Gene expression
Microarrays
Decidualization
UIII cells
description During the preimplantation phase of pregnancy the endometrial stroma differentiates into decidua, a process that implies numerous morphological changes and is an example of physiological transdifferentiation. Here we show that UIII rat endometrial stromal cells cultured in the presence of calf serum acquired morphological features of decidual cells and expressed decidual markers. To identify genes involved in decidualization we compared gene expression patterns of control and decidualized UIII cells using cDNA microarray. We found 322 annotated genes exhibiting significant differences in expression (>3-fold, fold discovery rate (FDR) >0.005), of which 312 have not been previously related to decidualization. Analysis of overrepresented functions revealed that protein synthesis, gene expression, and chromatin architecture and remodeling are the most relevant modified functions during decidualization. Relevant genes are also found in the functional terms differentiation, cell proliferation, signal transduction, and matrix/structural proteins. Several of these new genes involved in decidualization (Csdc2, Trim27, Eef1a1, Bmp1, Wt1, Aes, Gna12, and Men1) are shown to be also regulated in uterine decidua during normal pregnancy. Thus, the UIII cell culture model will allow future mechanistic studies to define the transcriptional network regulating reprogramming of stromal cells into decidual cells.
format Articulo
Preprint
author Vallejo, Griselda
Maschi, Darío
Mestre Citrinovitz, Ana Cecilia
Aiba, Kazuhiro
Maronna, Ricardo Antonio
Yohai, Victor
Ko, Minoru S.H.
Beato, Miguel
Saragüeta, Patricia
author_facet Vallejo, Griselda
Maschi, Darío
Mestre Citrinovitz, Ana Cecilia
Aiba, Kazuhiro
Maronna, Ricardo Antonio
Yohai, Victor
Ko, Minoru S.H.
Beato, Miguel
Saragüeta, Patricia
author_sort Vallejo, Griselda
title Changes in global gene expression during in vitro decidualization of rat endometrial stromal cells
title_short Changes in global gene expression during in vitro decidualization of rat endometrial stromal cells
title_full Changes in global gene expression during in vitro decidualization of rat endometrial stromal cells
title_fullStr Changes in global gene expression during in vitro decidualization of rat endometrial stromal cells
title_full_unstemmed Changes in global gene expression during in vitro decidualization of rat endometrial stromal cells
title_sort changes in global gene expression during in vitro decidualization of rat endometrial stromal cells
publishDate 2010
url http://sedici.unlp.edu.ar/handle/10915/128408
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bdutipo_str Repositorios
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