Impact of sphingomyelin acyl chain (16:0 vs 24:1) on the interfacial properties of Langmuir monolayers: A PM-IRRAS study
Membrane structure is a key factor for the cell`s physiology, pathology, and therapy. Evaluating the importance of lipid species such as N-nervonoyl sphingomyelin (24:1-SM) -able to prevent phase separation- to membrane structuring remains a formidable challenge. This is the first report in which po...
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| Autores principales: | , , , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/128276 |
| Aporte de: |
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I19-R120-10915-128276 |
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| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Bioquímica Membrane rafts Cholesterol-sphingomyelin interactions Langmuir monolayers PM-IRRAS |
| spellingShingle |
Bioquímica Membrane rafts Cholesterol-sphingomyelin interactions Langmuir monolayers PM-IRRAS Vázquez, Romina Florencia Daza Millone, María Antonieta Pavinatto, Felippe José Fanani, Maria Laura Oliveira, Osvaldo N. Vela, María Elena Maté, Sabina María Impact of sphingomyelin acyl chain (16:0 vs 24:1) on the interfacial properties of Langmuir monolayers: A PM-IRRAS study |
| topic_facet |
Bioquímica Membrane rafts Cholesterol-sphingomyelin interactions Langmuir monolayers PM-IRRAS |
| description |
Membrane structure is a key factor for the cell`s physiology, pathology, and therapy. Evaluating the importance of lipid species such as N-nervonoyl sphingomyelin (24:1-SM) -able to prevent phase separation- to membrane structuring remains a formidable challenge. This is the first report in which polarization-modulated infrared reflection-absorption spectroscopy (PM-IRRAS) is applied to investigate the lipid-lipid interactions in 16:0 vs 24:1-SM monolayers and their mixtures with 1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC) and cholesterol (Chol) (DOPC/SM/Chol 2:1:1). From the results we inferred that the cis double bond (Δ15) in 24:1-SM molecule diminishes intermolecular H-bonding and chain packing density compared to that of 16:0-SM. In ternary mixtures containing 16:0-SM, the relative intensity of the two components of the Amide I band reflected changes in the H-bonding network due to SM-Chol interactions. In contrast, the contribution of the main components of the Amide I band in DOPC/24:1-SM/Chol remained as in 24:1-SM monolayers, with a larger contribution of the non-H-bonded component. The most interesting feature in these ternary films is that the CO stretching mode of DOPC appeared with an intensity similar to that of SM Amide I band in DOPC/16:0-SM/Chol monolayers (a two-phase [Lo/Le] system), whereas an extremely low intensity of the CO band was detected in DOPC/24:1-SM/Chol monolayers (single Le phase). This is evidence that the unsaturation in 24:1-SM affected not only the conformational properties of acyl chains but also the orientation of the chemical groups at the air/water interface. The physical properties and overall H-bonding ability conferred by 24:1-SM may have implications in cell signaling and binding of biomolecules. |
| format |
Articulo Articulo |
| author |
Vázquez, Romina Florencia Daza Millone, María Antonieta Pavinatto, Felippe José Fanani, Maria Laura Oliveira, Osvaldo N. Vela, María Elena Maté, Sabina María |
| author_facet |
Vázquez, Romina Florencia Daza Millone, María Antonieta Pavinatto, Felippe José Fanani, Maria Laura Oliveira, Osvaldo N. Vela, María Elena Maté, Sabina María |
| author_sort |
Vázquez, Romina Florencia |
| title |
Impact of sphingomyelin acyl chain (16:0 vs 24:1) on the interfacial properties of Langmuir monolayers: A PM-IRRAS study |
| title_short |
Impact of sphingomyelin acyl chain (16:0 vs 24:1) on the interfacial properties of Langmuir monolayers: A PM-IRRAS study |
| title_full |
Impact of sphingomyelin acyl chain (16:0 vs 24:1) on the interfacial properties of Langmuir monolayers: A PM-IRRAS study |
| title_fullStr |
Impact of sphingomyelin acyl chain (16:0 vs 24:1) on the interfacial properties of Langmuir monolayers: A PM-IRRAS study |
| title_full_unstemmed |
Impact of sphingomyelin acyl chain (16:0 vs 24:1) on the interfacial properties of Langmuir monolayers: A PM-IRRAS study |
| title_sort |
impact of sphingomyelin acyl chain (16:0 vs 24:1) on the interfacial properties of langmuir monolayers: a pm-irras study |
| publishDate |
2018 |
| url |
http://sedici.unlp.edu.ar/handle/10915/128276 |
| work_keys_str_mv |
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Repositorios |
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1764820452216143876 |