Biosynthesis of anti-HCV compounds using thermophilic microorganisms

This work describes the application of thermophilic microorganisms for obtaining 6-halogenated purine nucleosides. Biosynthesis of 6-chloropurine-2′-deoxyriboside and 6-chloropurine riboside was achieved by Geobacillus stearothermophilus CECT 43 with a conversion of 90% and 68%, respectively. Furthe...

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Detalles Bibliográficos
Autores principales: Rivero, Cintia W., De Benedetti, Eliana C., Sambeth, Jorge Enrique, Lozano, Mario Enrique, Trelles, Jorge A.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2012
Materias:
Química
Geobacillus stearothermophilus
Nucleoside analogues
Green chemistry
Entrapment immobilization
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/128236
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
Descripción
Sumario:This work describes the application of thermophilic microorganisms for obtaining 6-halogenated purine nucleosides. Biosynthesis of 6-chloropurine-2′-deoxyriboside and 6-chloropurine riboside was achieved by Geobacillus stearothermophilus CECT 43 with a conversion of 90% and 68%, respectively. Furthermore, the selected microorganism was satisfactorily stabilized by immobilization in an agarose matrix. This biocatalyst can be reused at least 70 times without significant loss of activity, obtaining 379 mg/L of 6-chloropurine-2′-deoxyriboside. The obtained compounds can be used as antiviral agents.

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