Characterization of paneth cells in alpacas (<i>Vicugna pacos</i>, Mammalia, Camelidae)

Paneth cells are secretory epithelial cells of the innate immune system of the intestine of several mammals, including alpacas. Little is known about the latter; thus, in the present study we described the morphology and histochemical characteristics of Paneth cells in healthy fetuses, and young and...

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Autores principales: Vasquez, María, Lira, Boris, Rodríguez, José, Falcón, Néstor, Ocampo, Jorge Dario, Nishida, Fabián, Barbeito, Claudio Gustavo, Zanuzzi, Carolina Natalia
Formato: Articulo
Lenguaje:Inglés
Publicado: 2016
Materias:
Ciencias Veterinarias
Paneth cells
Alpacas
Morphometry
Immunohistochemistry
Lectinhistochemistry
Lactancy
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/128231
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-128231
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Paneth cells
Alpacas
Morphometry
Immunohistochemistry
Lectinhistochemistry
Lactancy
spellingShingle Ciencias Veterinarias
Paneth cells
Alpacas
Morphometry
Immunohistochemistry
Lectinhistochemistry
Lactancy
Vasquez, María
Lira, Boris
Rodríguez, José
Falcón, Néstor
Ocampo, Jorge Dario
Nishida, Fabián
Barbeito, Claudio Gustavo
Zanuzzi, Carolina Natalia
Characterization of paneth cells in alpacas (<i>Vicugna pacos</i>, Mammalia, Camelidae)
topic_facet Ciencias Veterinarias
Paneth cells
Alpacas
Morphometry
Immunohistochemistry
Lectinhistochemistry
Lactancy
description Paneth cells are secretory epithelial cells of the innate immune system of the intestine of several mammals, including alpacas. Little is known about the latter; thus, in the present study we described the morphology and histochemical characteristics of Paneth cells in healthy fetuses, and young and adult alpacas. For this purpose, samples of duodenum, jejunum and ileum were taken from 6 fetuses at different days of pregnancy (between days 221-330), 66 offsprings (between 0 and 45-days-old) and 5 adult alpacas (>2-years-old). Samples were fixed in 10% buffered formalin and processed for histological and morphometrical analysis using HE and Masson Trichomics technique. Immunohistochemistry was used to identify Paneth cells using anti-lysozyme antibody. In addition, the lectinhistochemichal binding-pattern of Paneth celĺs granules was evaluated. Lyzozyme was immunohistochemically detected in the granules of Paneth cells from day 283 of pregnancy in all the small intestinal sections of the studied fetuses. In newborn alpacas Paneth cells were initially found in the duodenum, but the following days (days 18-21 after birth) they were also found in the ileum. Their size gradually increased after birth, but then no significant differences were found. In adult alpacas the number was lower than offsprings. We suggest that Paneth cells early differentiate in the small intestine of alpacas, and the increase in their number during the first two weeks of life strongly support their possible involvement in the intestinal defensive functions against the enteric diseases that occur during the lactancy stage.
format Articulo
Articulo
author Vasquez, María
Lira, Boris
Rodríguez, José
Falcón, Néstor
Ocampo, Jorge Dario
Nishida, Fabián
Barbeito, Claudio Gustavo
Zanuzzi, Carolina Natalia
author_facet Vasquez, María
Lira, Boris
Rodríguez, José
Falcón, Néstor
Ocampo, Jorge Dario
Nishida, Fabián
Barbeito, Claudio Gustavo
Zanuzzi, Carolina Natalia
author_sort Vasquez, María
title Characterization of paneth cells in alpacas (<i>Vicugna pacos</i>, Mammalia, Camelidae)
title_short Characterization of paneth cells in alpacas (<i>Vicugna pacos</i>, Mammalia, Camelidae)
title_full Characterization of paneth cells in alpacas (<i>Vicugna pacos</i>, Mammalia, Camelidae)
title_fullStr Characterization of paneth cells in alpacas (<i>Vicugna pacos</i>, Mammalia, Camelidae)
title_full_unstemmed Characterization of paneth cells in alpacas (<i>Vicugna pacos</i>, Mammalia, Camelidae)
title_sort characterization of paneth cells in alpacas (<i>vicugna pacos</i>, mammalia, camelidae)
publishDate 2016
url http://sedici.unlp.edu.ar/handle/10915/128231
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