Expression of Ghrelin and Its Receptor mRNA in Bovine Oocyte and Cumulus Cells

Energy balance is regulated by ghrelin which is a neuroendocrine modulator. Ghrelin is expressed in reproductive organs. However, the role of ghrelin during <i>in vitro</i> maturation (IVM) and bovine preimplantational development is limited. The purpose of this study was to measure the...

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Detalles Bibliográficos
Autores principales: Sirini, Matías Ángel, Anchordoquy, Juan Patricio, Quintana, Silvina, Furnus, Cecilia Cristina, Relling, Alejandro Enrique, Anchordoquy, Juan Mateo
Formato: Articulo
Lenguaje:Inglés
Publicado: 2019
Materias:
Veterinaria
Ghrelin
GHS-R1A
In Vitro Oocyte Maturation
mRNA Expression
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/128216
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-128216
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Veterinaria
Ghrelin
GHS-R1A
In Vitro Oocyte Maturation
mRNA Expression
spellingShingle Veterinaria
Ghrelin
GHS-R1A
In Vitro Oocyte Maturation
mRNA Expression
Sirini, Matías Ángel
Anchordoquy, Juan Patricio
Quintana, Silvina
Furnus, Cecilia Cristina
Relling, Alejandro Enrique
Anchordoquy, Juan Mateo
Expression of Ghrelin and Its Receptor mRNA in Bovine Oocyte and Cumulus Cells
topic_facet Veterinaria
Ghrelin
GHS-R1A
In Vitro Oocyte Maturation
mRNA Expression
description Energy balance is regulated by ghrelin which is a neuroendocrine modulator. Ghrelin is expressed in reproductive organs. However, the role of ghrelin during <i>in vitro</i> maturation (IVM) and bovine preimplantational development is limited. The purpose of this study was to measure the expression of ghrelin (GHRL) and its receptor growth hormone secretagogue receptor 1A (GHS-R1A) mRNA, and determine cumulus oocyte complex (COC) viability after IVM with 0, 20, 40 and 60 pM of ghrelin. Also, pronuclear formation was recorded after <i>in vitro</i> fertilization (IVF). GHRL and GHS-R1A mRNA expression in oocyte and cumulus cells (CCs) was assessed using reverse transcription-polymerase chain reaction (PCR). Oocyte and CC viability were analyzed with the fluorescein diacetate fluorochrome-trypan blue technique. Pronuclear formation was determined 18 hours after IVF with Hoechst 33342. The results demonstrated that ghrelin mRNA is present in oocyte and CCs before and after 24 hours IVM with all treatments. Ghrelin receptor, GHS-R1A, was only detected in oocytes and CCs after 24 hours IVM with 20, 40 and 60 pM of ghrelin. Oocyte viability was not significantly different (P=0.77) among treatments. However, CC viability was significantly lower (P=0.04) when COCs were matured with ghrelin (77.65, 72.10, 66.32 and 46.86% for 0, 20, 40, and 60 pM of ghrelin, respectively). The chance of two pronuclei forming were higher (P=0.03) when ghrelin was not be added to the IVM medium. We found that ghrelin negatively impacts CC viability and pronuclear formation.
format Articulo
Articulo
author Sirini, Matías Ángel
Anchordoquy, Juan Patricio
Quintana, Silvina
Furnus, Cecilia Cristina
Relling, Alejandro Enrique
Anchordoquy, Juan Mateo
author_facet Sirini, Matías Ángel
Anchordoquy, Juan Patricio
Quintana, Silvina
Furnus, Cecilia Cristina
Relling, Alejandro Enrique
Anchordoquy, Juan Mateo
author_sort Sirini, Matías Ángel
title Expression of Ghrelin and Its Receptor mRNA in Bovine Oocyte and Cumulus Cells
title_short Expression of Ghrelin and Its Receptor mRNA in Bovine Oocyte and Cumulus Cells
title_full Expression of Ghrelin and Its Receptor mRNA in Bovine Oocyte and Cumulus Cells
title_fullStr Expression of Ghrelin and Its Receptor mRNA in Bovine Oocyte and Cumulus Cells
title_full_unstemmed Expression of Ghrelin and Its Receptor mRNA in Bovine Oocyte and Cumulus Cells
title_sort expression of ghrelin and its receptor mrna in bovine oocyte and cumulus cells
publishDate 2019
url http://sedici.unlp.edu.ar/handle/10915/128216
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