The stay-green phenotype of TaNAM-RNAi wheat plants is associated with maintenance of chloroplast structure and high enzymatic antioxidant activity

TaNAM transcription factors play an important role in controlling senescence, which in turn, influences the delivery of nitrogen, iron and other elements to the grain of wheat (Triticum aestivum) plants, thus contributing to grain nutritional value. While lack or diminished expression of TaNAMs dete...

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Autores principales: Checovich, Mariana Leonela, Galatro, Andrea Verónica, Moriconi, Jorge I., Simontacchi, Marcela, Dubcovsky, Jorge, Santa-María, Guillermo E.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2016
Materias:
Bioquímica
Botánica
Chloroplast
NAM
Senescence
Stay-green
Triticum aestivum
Wheat
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/127948
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-127948
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Bioquímica
Botánica
Chloroplast
NAM
Senescence
Stay-green
Triticum aestivum
Wheat
spellingShingle Bioquímica
Botánica
Chloroplast
NAM
Senescence
Stay-green
Triticum aestivum
Wheat
Checovich, Mariana Leonela
Galatro, Andrea Verónica
Moriconi, Jorge I.
Simontacchi, Marcela
Dubcovsky, Jorge
Santa-María, Guillermo E.
The stay-green phenotype of TaNAM-RNAi wheat plants is associated with maintenance of chloroplast structure and high enzymatic antioxidant activity
topic_facet Bioquímica
Botánica
Chloroplast
NAM
Senescence
Stay-green
Triticum aestivum
Wheat
description TaNAM transcription factors play an important role in controlling senescence, which in turn, influences the delivery of nitrogen, iron and other elements to the grain of wheat (Triticum aestivum) plants, thus contributing to grain nutritional value. While lack or diminished expression of TaNAMs determines a stay-green phenotype, the precise effect of these factors on chloroplast structure has not been studied. In this work we focused on the events undergone by chloroplasts in two wheat lines having either control or diminished TaNAM expression due to RNA interference (RNAi). It was found that in RNAi plants maintenance of chlorophyll levels and maximal photochemical efficiency of photosystem II were associated with lack of chloroplast dismantling. Flow cytometer studies and electron microscope analysis showed that RNAi plants conserved organelle ultrastructure and complexity. It was also found that senescence in control plants was accompanied by a low leaf enzymatic antioxidant activity. Lack of chloroplast dismantling in RNAi plants was associated with maintenance of protein and iron concentration in the flag leaf, the opposite being observed in control plants. These data provide a structural basis for the observation that down regulation of TaNAMs confers a functional stay-green phenotype and indicate that the low export of iron and nitrogen from the flag leaf of these plants is concomitant, within the developmental window studied, with lack of chloroplast degradation and high enzymatic antioxidant activity.
format Articulo
Articulo
author Checovich, Mariana Leonela
Galatro, Andrea Verónica
Moriconi, Jorge I.
Simontacchi, Marcela
Dubcovsky, Jorge
Santa-María, Guillermo E.
author_facet Checovich, Mariana Leonela
Galatro, Andrea Verónica
Moriconi, Jorge I.
Simontacchi, Marcela
Dubcovsky, Jorge
Santa-María, Guillermo E.
author_sort Checovich, Mariana Leonela
title The stay-green phenotype of TaNAM-RNAi wheat plants is associated with maintenance of chloroplast structure and high enzymatic antioxidant activity
title_short The stay-green phenotype of TaNAM-RNAi wheat plants is associated with maintenance of chloroplast structure and high enzymatic antioxidant activity
title_full The stay-green phenotype of TaNAM-RNAi wheat plants is associated with maintenance of chloroplast structure and high enzymatic antioxidant activity
title_fullStr The stay-green phenotype of TaNAM-RNAi wheat plants is associated with maintenance of chloroplast structure and high enzymatic antioxidant activity
title_full_unstemmed The stay-green phenotype of TaNAM-RNAi wheat plants is associated with maintenance of chloroplast structure and high enzymatic antioxidant activity
title_sort stay-green phenotype of tanam-rnai wheat plants is associated with maintenance of chloroplast structure and high enzymatic antioxidant activity
publishDate 2016
url http://sedici.unlp.edu.ar/handle/10915/127948
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