Cellular injuries of spray-dried <i>Lactobacillus</i> spp. isolated from kefir and their impact on probiotic properties

The injuries caused by spray drying (SD) of three potential probiotic lactobacilli isolated from kefir grains and the impact on some probiotic properties, were evaluated. Results demonstrated that Lactobacillus plantarum 83114 and L. kefir 8321 showed a slight reduction of viability (0.11 and 0.29 l...

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Autores principales: Golowczyc, Marina Alejandra, Silva, Joana, Teixeira, Paula, De Antoni, Graciela Liliana, Abraham, Analía Graciela
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2010
Materias:
Biología
Lactobacillus spp.
Kéfir
Spray drying
Probiotic properties
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/127936
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-127936
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Lactobacillus spp.
Kéfir
Spray drying
Probiotic properties
spellingShingle Biología
Lactobacillus spp.
Kéfir
Spray drying
Probiotic properties
Golowczyc, Marina Alejandra
Silva, Joana
Teixeira, Paula
De Antoni, Graciela Liliana
Abraham, Analía Graciela
Cellular injuries of spray-dried <i>Lactobacillus</i> spp. isolated from kefir and their impact on probiotic properties
topic_facet Biología
Lactobacillus spp.
Kéfir
Spray drying
Probiotic properties
description The injuries caused by spray drying (SD) of three potential probiotic lactobacilli isolated from kefir grains and the impact on some probiotic properties, were evaluated. Results demonstrated that Lactobacillus plantarum 83114 and L. kefir 8321 showed a slight reduction of viability (0.11 and 0.29 log CFU/ml respectively) after SD process, and L. kefir 8348 was found to be more sensitive to the process with a reduction in viability of 0.70 log CFU/ml. Neither membrane damage, evaluated by increased sensitivity to NaCl, lysozyme, bile salt and penicillin G, nor changes in acidifying activity in MRS and milk by lactobacilli were detected after SD. L. plantarum 83114 and L. kefir 8321 after SD did not lose their capacity to adhere to intestinal cells. Nevertheless, L. kefir 8348 showed a significant loss of adhesion capacity after SD. In addition, rehydrated spray-dried L. kefir 8321 retained the ability to protect against Salmonella invasion of intestinal cells. This effect was observed when L. kefir is co-incubated with Salmonella before invasion assay. This work shows that the membrane integrity evaluated by indirect methods and some probiotic properties of lactobacilli isolated from kefir did not change significantly after SD, and these powders could be used in functional foods applications.
format Articulo
Preprint
author Golowczyc, Marina Alejandra
Silva, Joana
Teixeira, Paula
De Antoni, Graciela Liliana
Abraham, Analía Graciela
author_facet Golowczyc, Marina Alejandra
Silva, Joana
Teixeira, Paula
De Antoni, Graciela Liliana
Abraham, Analía Graciela
author_sort Golowczyc, Marina Alejandra
title Cellular injuries of spray-dried <i>Lactobacillus</i> spp. isolated from kefir and their impact on probiotic properties
title_short Cellular injuries of spray-dried <i>Lactobacillus</i> spp. isolated from kefir and their impact on probiotic properties
title_full Cellular injuries of spray-dried <i>Lactobacillus</i> spp. isolated from kefir and their impact on probiotic properties
title_fullStr Cellular injuries of spray-dried <i>Lactobacillus</i> spp. isolated from kefir and their impact on probiotic properties
title_full_unstemmed Cellular injuries of spray-dried <i>Lactobacillus</i> spp. isolated from kefir and their impact on probiotic properties
title_sort cellular injuries of spray-dried <i>lactobacillus</i> spp. isolated from kefir and their impact on probiotic properties
publishDate 2010
url http://sedici.unlp.edu.ar/handle/10915/127936
work_keys_str_mv AT golowczycmarinaalejandra cellularinjuriesofspraydriedilactobacillusisppisolatedfromkefirandtheirimpactonprobioticproperties
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AT teixeirapaula cellularinjuriesofspraydriedilactobacillusisppisolatedfromkefirandtheirimpactonprobioticproperties
AT deantonigracielaliliana cellularinjuriesofspraydriedilactobacillusisppisolatedfromkefirandtheirimpactonprobioticproperties
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bdutipo_str Repositorios
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