Non-Muscle Myosin IIA (Myh9) is in the Nucleus of S-Phase Entering NT2-D1 Cells

Non-muscle myosin IIA is a cytoplasmic protein that works in concert with F-actin to produce cell movement. The heavy chain of this protein is codified by the MYH9 gene. The presence of motor proteins as myosin or mono and F-actin and their role in transcription has recently been observed. Prep1–the...

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Detalles Bibliográficos
Autores principales: Naum-Onganía, Gabriela, Rivera Pomar, Rolando Víctor
Formato: Articulo
Lenguaje:Inglés
Publicado: 2018
Materias:
Biología
Myosin9
Hox genes
NT2-D1 cells
S-phase
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/127843
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-127843
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Myosin9
Hox genes
NT2-D1 cells
S-phase
spellingShingle Biología
Myosin9
Hox genes
NT2-D1 cells
S-phase
Naum-Onganía, Gabriela
Rivera Pomar, Rolando Víctor
Non-Muscle Myosin IIA (Myh9) is in the Nucleus of S-Phase Entering NT2-D1 Cells
topic_facet Biología
Myosin9
Hox genes
NT2-D1 cells
S-phase
description Non-muscle myosin IIA is a cytoplasmic protein that works in concert with F-actin to produce cell movement. The heavy chain of this protein is codified by the MYH9 gene. The presence of motor proteins as myosin or mono and F-actin and their role in transcription has recently been observed. Prep1–the transcription factor of HOXB genes– constitutes a dimer with Pbx1, which induces HOXB gene expression. Prep1 has been found purifying with β-actin and Myh9. HOXB transcription initiates when cells enter in S-phase, during which DNA duplication and transcription occur at the same time. Here, we have shown that Myh9 co-localizes with Prep1 in the nucleus and in the periphery of the nucleolus in S-phase NT2-D1 cells. Furthermore, we have shown that Myh9 purifies with Pbx1 from nuclear extracts of S-phase entering NT2-D1 cells –and not from cytoplasmic extracts. Taking into account these results, we conclude that Myh9 is in the nucleus of the S-phase entering NT2-D1 cells and might have a role in HOXB transcription.
format Articulo
Articulo
author Naum-Onganía, Gabriela
Rivera Pomar, Rolando Víctor
author_facet Naum-Onganía, Gabriela
Rivera Pomar, Rolando Víctor
author_sort Naum-Onganía, Gabriela
title Non-Muscle Myosin IIA (Myh9) is in the Nucleus of S-Phase Entering NT2-D1 Cells
title_short Non-Muscle Myosin IIA (Myh9) is in the Nucleus of S-Phase Entering NT2-D1 Cells
title_full Non-Muscle Myosin IIA (Myh9) is in the Nucleus of S-Phase Entering NT2-D1 Cells
title_fullStr Non-Muscle Myosin IIA (Myh9) is in the Nucleus of S-Phase Entering NT2-D1 Cells
title_full_unstemmed Non-Muscle Myosin IIA (Myh9) is in the Nucleus of S-Phase Entering NT2-D1 Cells
title_sort non-muscle myosin iia (myh9) is in the nucleus of s-phase entering nt2-d1 cells
publishDate 2018
url http://sedici.unlp.edu.ar/handle/10915/127843
work_keys_str_mv AT naumonganiagabriela nonmusclemyosiniiamyh9isinthenucleusofsphaseenteringnt2d1cells
AT riverapomarrolandovictor nonmusclemyosiniiamyh9isinthenucleusofsphaseenteringnt2d1cells
bdutipo_str Repositorios
_version_ 1764820451399303170

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