Interactions with membrane lipids and SNARE complex underlie dual roles for calcium-dependent activator protein for secretion (CAPS) in large dense-core vesicles (LDCVS) priming

CAPS (Calcium-dependent activator protein for secretion), was discovered as a soluble factor required for catecholamine secretion from PC12 cells. CAPS facilitates release of dense-core vesicles and release of neurotransmitters. In vertebrates CAPS is required for priming of synaptic vesicles and de...

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Detalles Bibliográficos
Autores principales: Stevens, David R., Ratai, Olga, Rettig, Jens
Formato: Articulo Revision
Lenguaje:Inglés
Publicado: 2015
Materias:
Ciencias Médicas
priming
exocytosis
synaptic vesicles
large dense-core vesicles
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/127787
https://pmr.safisiol.org.ar/archive/id/79
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-127787
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
priming
exocytosis
synaptic vesicles
large dense-core vesicles
spellingShingle Ciencias Médicas
priming
exocytosis
synaptic vesicles
large dense-core vesicles
Stevens, David R.
Ratai, Olga
Rettig, Jens
Interactions with membrane lipids and SNARE complex underlie dual roles for calcium-dependent activator protein for secretion (CAPS) in large dense-core vesicles (LDCVS) priming
topic_facet Ciencias Médicas
priming
exocytosis
synaptic vesicles
large dense-core vesicles
description CAPS (Calcium-dependent activator protein for secretion), was discovered as a soluble factor required for catecholamine secretion from PC12 cells. CAPS facilitates release of dense-core vesicles and release of neurotransmitters. In vertebrates CAPS is required for priming of synaptic vesicles and dense-core vesicles in neurons and in catecholamine release from the adrenal medulla. The pleckstrin homology domain of CAPS has been shown to mediate an interaction with the plasma membrane. An interaction with SNARE proteins and assembled SNARE complexes requires the Munc13 homology domain. The interaction with SNARE components facilitates SNARE complex formation, the molecular basis of the priming reaction, while interactions with the phosphoinositol 4,5 bisphosphate (PI(4,5)P2) rich areas of the plasma membrane localize CAPS at the release site but may also promote the fusion of primed vesicles. CAPS splice variants lacking the Munc13 homology domain but with an intact pleckstrin homology domain promote priming to the readily releasable pool while CAPS splice variants with a deletion in the pleckstrin homology domain promote catecholamine release, albeit at slower rates. These results confirm a dual action of CAPS dependent on the Munc13 homology- and the pleckstrin homology domains, respectively.
format Articulo
Revision
author Stevens, David R.
Ratai, Olga
Rettig, Jens
author_facet Stevens, David R.
Ratai, Olga
Rettig, Jens
author_sort Stevens, David R.
title Interactions with membrane lipids and SNARE complex underlie dual roles for calcium-dependent activator protein for secretion (CAPS) in large dense-core vesicles (LDCVS) priming
title_short Interactions with membrane lipids and SNARE complex underlie dual roles for calcium-dependent activator protein for secretion (CAPS) in large dense-core vesicles (LDCVS) priming
title_full Interactions with membrane lipids and SNARE complex underlie dual roles for calcium-dependent activator protein for secretion (CAPS) in large dense-core vesicles (LDCVS) priming
title_fullStr Interactions with membrane lipids and SNARE complex underlie dual roles for calcium-dependent activator protein for secretion (CAPS) in large dense-core vesicles (LDCVS) priming
title_full_unstemmed Interactions with membrane lipids and SNARE complex underlie dual roles for calcium-dependent activator protein for secretion (CAPS) in large dense-core vesicles (LDCVS) priming
title_sort interactions with membrane lipids and snare complex underlie dual roles for calcium-dependent activator protein for secretion (caps) in large dense-core vesicles (ldcvs) priming
publishDate 2015
url http://sedici.unlp.edu.ar/handle/10915/127787
https://pmr.safisiol.org.ar/archive/id/79
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