Changes in microbial communities during phytoremediation of contaminated soil with phenanthrene

Although polycyclic aromatic hydrocarbons (PAHs) are environmental pollutants that affect negatively soils biology, several strategies lead to their removal such as the phytoremediation. In order to assess the potential of phytoremediation using “alfalfa” Medicago sativa as a strategy to reduce the...

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Autores principales: Hariyo, Diego Daniel, Saparrat, Mario Carlos Nazareno, Barrera, Marcelo Daniel
Formato: Articulo
Lenguaje:Inglés
Publicado: 2020
Materias:
Biología
Botánica
Bioremediation
PAHs
Rhizosphere
Rhizodegradation
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/127322
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-127322
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Botánica
Bioremediation
PAHs
Rhizosphere
Rhizodegradation
spellingShingle Biología
Botánica
Bioremediation
PAHs
Rhizosphere
Rhizodegradation
Hariyo, Diego Daniel
Saparrat, Mario Carlos Nazareno
Barrera, Marcelo Daniel
Changes in microbial communities during phytoremediation of contaminated soil with phenanthrene
topic_facet Biología
Botánica
Bioremediation
PAHs
Rhizosphere
Rhizodegradation
description Although polycyclic aromatic hydrocarbons (PAHs) are environmental pollutants that affect negatively soils biology, several strategies lead to their removal such as the phytoremediation. In order to assess the potential of phytoremediation using “alfalfa” Medicago sativa as a strategy to reduce the phenanthrene on the soil, we analyzed the structure and dynamic of the microbial communities of a microcosm soil artificially contaminated with phenanthrene (2000 ppm), which was exposed to the plants. At different incubation times (7, 14, 21, 28, 42, and 56 days), a soil sample was taken from each microcosm and the residual amount of phenanthrene was quantified. Dehydrogenase activity and the count of fungi and bacteria were also estimated. Bacterial communities were characterized using PCR-DGGE, Shannon and Weaver’s indexes, multivariate analysis, and rarefaction curves. It was found that phytoremediation treatment was associated with a higher richness and bacterial diversity compared with those on control soil. Although an OTUs (Operational Taxonomic Unit) succession over time was detected in both treatments, bacterial richness and diversity were conditioned by the phenanthrene concentration available and also dependent on the treatment, which were associated to different bacterial communities. In this study, phytoremediation treatment reduced the content of phenanthrene in the soil after 56 days to a 0.45% compared with the control treatment, which only reached to 4.25%. This preliminary work suggests the promoting activity of “alfalfa” plants, through rhizodegradation, to remove in soil PAHs, as well as its relevance in the activation of different ecological processes mediated by soil microorganisms.
format Articulo
Articulo
author Hariyo, Diego Daniel
Saparrat, Mario Carlos Nazareno
Barrera, Marcelo Daniel
author_facet Hariyo, Diego Daniel
Saparrat, Mario Carlos Nazareno
Barrera, Marcelo Daniel
author_sort Hariyo, Diego Daniel
title Changes in microbial communities during phytoremediation of contaminated soil with phenanthrene
title_short Changes in microbial communities during phytoremediation of contaminated soil with phenanthrene
title_full Changes in microbial communities during phytoremediation of contaminated soil with phenanthrene
title_fullStr Changes in microbial communities during phytoremediation of contaminated soil with phenanthrene
title_full_unstemmed Changes in microbial communities during phytoremediation of contaminated soil with phenanthrene
title_sort changes in microbial communities during phytoremediation of contaminated soil with phenanthrene
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/127322
work_keys_str_mv AT hariyodiegodaniel changesinmicrobialcommunitiesduringphytoremediationofcontaminatedsoilwithphenanthrene
AT saparratmariocarlosnazareno changesinmicrobialcommunitiesduringphytoremediationofcontaminatedsoilwithphenanthrene
AT barreramarcelodaniel changesinmicrobialcommunitiesduringphytoremediationofcontaminatedsoilwithphenanthrene
bdutipo_str Repositorios
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