Molecular determinants of disease in coxsackievirus B1 murine infection

To understand better how different genomic regions may confer pathogenicity for the coxsackievirus B (CVB), two intratypic CVB1 variants and a number of recombinant viruses were studied. Sequencing analysis showed 23 nucleotide changes between the parental non-pathogenic CVB1N and the pathogenic CVB...

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Autores principales: Cifuente, Javier O., Ferrer, María Florencia, Jaquenod De Giusti, Carolina, Song, Wen Chao, Romanowski, Víctor, Hafenstein, Susan, Gómez, Ricardo Martín
Formato: Articulo
Lenguaje:Inglés
Publicado: 2011
Materias:
Ciencias Exactas
Química
pancreatitis
myocarditis
DAF
CAR
mice
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/127313
https://onlinelibrary.wiley.com/doi/10.1002/jmv.22133?__cf_chl_jschl_tk__=pmd_TYe1oxqEE5xuOqdGUt3a4GPE9MkF4Wt2_ZxBPatX4fo-1635269542-0-gqNtZGzNAiWjcnBszQtR
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SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-127313
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Química
pancreatitis
myocarditis
DAF
CAR
mice
spellingShingle Ciencias Exactas
Química
pancreatitis
myocarditis
DAF
CAR
mice
Cifuente, Javier O.
Ferrer, María Florencia
Jaquenod De Giusti, Carolina
Song, Wen Chao
Romanowski, Víctor
Hafenstein, Susan
Gómez, Ricardo Martín
Molecular determinants of disease in coxsackievirus B1 murine infection
topic_facet Ciencias Exactas
Química
pancreatitis
myocarditis
DAF
CAR
mice
description To understand better how different genomic regions may confer pathogenicity for the coxsackievirus B (CVB), two intratypic CVB1 variants and a number of recombinant viruses were studied. Sequencing analysis showed 23 nucleotide changes between the parental non-pathogenic CVB1N and the pathogenic CVB1Nm. Mutations present in CVB1Nm were more conserved than those in CVB1N when compared to other CVB sequences. Inoculation in C3H/HeJ mice showed that the P1 region is critical for pathogenicity in murine pancreas and heart. The molecular determinants of disease for these organs partially overlap. Several P1 region amino acid differences appear to be located in the decay accelerating factor (DAF) footprint CVBs. CVB1N and CVB1Nm interacted with human CAR, but only CVB1N seemed to interact with human DAF, as determined using soluble receptors in a plaque reduction assay. However, the murine homologue Daf-1 did not interact with any virus assessed by haemagglutination. The results of this study suggest that an unknown receptor interaction with the virus play an important role in the pathogenicity of CVB1Nm. Further <i>in vivo</i> studies may clarify this issue.
format Articulo
Articulo
author Cifuente, Javier O.
Ferrer, María Florencia
Jaquenod De Giusti, Carolina
Song, Wen Chao
Romanowski, Víctor
Hafenstein, Susan
Gómez, Ricardo Martín
author_facet Cifuente, Javier O.
Ferrer, María Florencia
Jaquenod De Giusti, Carolina
Song, Wen Chao
Romanowski, Víctor
Hafenstein, Susan
Gómez, Ricardo Martín
author_sort Cifuente, Javier O.
title Molecular determinants of disease in coxsackievirus B1 murine infection
title_short Molecular determinants of disease in coxsackievirus B1 murine infection
title_full Molecular determinants of disease in coxsackievirus B1 murine infection
title_fullStr Molecular determinants of disease in coxsackievirus B1 murine infection
title_full_unstemmed Molecular determinants of disease in coxsackievirus B1 murine infection
title_sort molecular determinants of disease in coxsackievirus b1 murine infection
publishDate 2011
url http://sedici.unlp.edu.ar/handle/10915/127313
https://onlinelibrary.wiley.com/doi/10.1002/jmv.22133?__cf_chl_jschl_tk__=pmd_TYe1oxqEE5xuOqdGUt3a4GPE9MkF4Wt2_ZxBPatX4fo-1635269542-0-gqNtZGzNAiWjcnBszQtR
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