NanoPaint: a tool for rapid and dynamic imaging of membrane structural plasticity at the nanoscale
Single-particle tracking with quantum dots (QDs) constitutes a powerful tool to track the nanoscopic dynamics of individual cell membrane components unveiling their membrane diffusion characteristics. Here, the nano-resolved population dynamics of QDs is exploited to reconstruct the topography and s...
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Autores principales: | , , , , , |
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Formato: | Articulo |
Lenguaje: | Inglés |
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2019
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Materias: | |
Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/127302 |
Aporte de: |
id |
I19-R120-10915-127302 |
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record_format |
dspace |
institution |
Universidad Nacional de La Plata |
institution_str |
I-19 |
repository_str |
R-120 |
collection |
SEDICI (UNLP) |
language |
Inglés |
topic |
Física Biophysics Membrane protein Super-resolution microscopy Materials science Transmembrane protein Cell membrane Population Synaptic cleft Membrane Filopodia Cannabinoid receptor type 1 Neuronal plasticity Quantum dots Synapses |
spellingShingle |
Física Biophysics Membrane protein Super-resolution microscopy Materials science Transmembrane protein Cell membrane Population Synaptic cleft Membrane Filopodia Cannabinoid receptor type 1 Neuronal plasticity Quantum dots Synapses Tasso, Mariana Pons, Thomas Lequeux, Nicolas Nguyen, Julie Lenkei, Zsolt Zala, Diana NanoPaint: a tool for rapid and dynamic imaging of membrane structural plasticity at the nanoscale |
topic_facet |
Física Biophysics Membrane protein Super-resolution microscopy Materials science Transmembrane protein Cell membrane Population Synaptic cleft Membrane Filopodia Cannabinoid receptor type 1 Neuronal plasticity Quantum dots Synapses |
description |
Single-particle tracking with quantum dots (QDs) constitutes a powerful tool to track the nanoscopic dynamics of individual cell membrane components unveiling their membrane diffusion characteristics. Here, the nano-resolved population dynamics of QDs is exploited to reconstruct the topography and structural changes of the cell membrane surface with high temporal and spatial resolution. For this proof-of-concept study, bright, small, and stable biofunctional QD nanoconstructs are utilized recognizing the endogenous neuronal cannabinoid receptor 1, a highly expressed and fast-diffusing membrane protein, together with a commercial point-localization microscope. Rapid QD diffusion on the axonal plasma membrane of cultured hippocampal neurons allows precise reconstruction of the membrane surface in less than 1 min with a spatial resolution of tens of nanometers. Access of the QD nanoconstructs to the synaptic cleft enables rapid 3D topological reconstruction of the entire presynaptic component. Successful reconstruction of membrane nano-topology and deformation at the second time-scale is also demonstrated for HEK293 cell filopodia and axons. Named "nanoPaint," this super-resolution imaging technique amenable to any endogenous transmembrane target represents a versatile platform to rapidly and accurately reconstruct the cell membrane nano-topography, thereby enabling the study of the rapid dynamic phenomena involved in neuronal membrane plasticity. |
format |
Articulo Articulo |
author |
Tasso, Mariana Pons, Thomas Lequeux, Nicolas Nguyen, Julie Lenkei, Zsolt Zala, Diana |
author_facet |
Tasso, Mariana Pons, Thomas Lequeux, Nicolas Nguyen, Julie Lenkei, Zsolt Zala, Diana |
author_sort |
Tasso, Mariana |
title |
NanoPaint: a tool for rapid and dynamic imaging of membrane structural plasticity at the nanoscale |
title_short |
NanoPaint: a tool for rapid and dynamic imaging of membrane structural plasticity at the nanoscale |
title_full |
NanoPaint: a tool for rapid and dynamic imaging of membrane structural plasticity at the nanoscale |
title_fullStr |
NanoPaint: a tool for rapid and dynamic imaging of membrane structural plasticity at the nanoscale |
title_full_unstemmed |
NanoPaint: a tool for rapid and dynamic imaging of membrane structural plasticity at the nanoscale |
title_sort |
nanopaint: a tool for rapid and dynamic imaging of membrane structural plasticity at the nanoscale |
publishDate |
2019 |
url |
http://sedici.unlp.edu.ar/handle/10915/127302 |
work_keys_str_mv |
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bdutipo_str |
Repositorios |
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1764820451557638146 |