In vitro experiments and infrared spectroscopy analysis of acid and alkaline phosphatase inhibition by vanadium complexes

In the course of our research on vanadium-containing complexes, two oxidovanadium complexes containing vanadium(V) and (IV) cores with 4-aminobenzoic acid and/or the peroxo anion as ligands were synthesized and characterized by elemental analysis, conductivity measurements, thermogravimetric analysi...

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Autores principales: Parente, Juliana Elena, Naso, Luciana, Jori, Khalil, Franca, Carlos Alberto, da Costa Ferreira, Ana María, Williams, Patricia Ana María, Ferrer, Evelina Gloria
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2019
Materias:
Ciencias Exactas
Química
vanadium complexes
phosphatase inhibition
FTIR spectroscopy
secondary
structure
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/127267
https://pubs.rsc.org/en/content/articlelanding/2019/NJ/C9NJ01638D
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-127267
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Química
vanadium complexes
phosphatase inhibition
FTIR spectroscopy
secondary
structure
spellingShingle Ciencias Exactas
Química
vanadium complexes
phosphatase inhibition
FTIR spectroscopy
secondary
structure
Parente, Juliana Elena
Naso, Luciana
Jori, Khalil
Franca, Carlos Alberto
da Costa Ferreira, Ana María
Williams, Patricia Ana María
Ferrer, Evelina Gloria
In vitro experiments and infrared spectroscopy analysis of acid and alkaline phosphatase inhibition by vanadium complexes
topic_facet Ciencias Exactas
Química
vanadium complexes
phosphatase inhibition
FTIR spectroscopy
secondary
structure
description In the course of our research on vanadium-containing complexes, two oxidovanadium complexes containing vanadium(V) and (IV) cores with 4-aminobenzoic acid and/or the peroxo anion as ligands were synthesized and characterized by elemental analysis, conductivity measurements, thermogravimetric analysis, and 1H NMR, EPR, FTIR, and UV/vis spectroscopies. Their compositions and geometrical structures ([VO(O<sub>2</sub>)(C<sub>7</sub>H<sub>6</sub>NO<sub>2</sub>)(H<sub>2</sub>O)]·H<sub>2</sub>O and [VO(C<sub>7</sub>H<sub>6</sub>NO<sub>2</sub>)<sub>2</sub>H<sub>2</sub>O]) were supported by experimental data and theoretical studies (density functional theory, DFT). The complexes were evaluated in vitro as phosphatase inhibitors (alkaline and acid enzymes), being considered as potential pharmaceutical agents under over-expression of those biochemical markers. The effect achieved was then analysed through FTIR spectroscopy. Changes in the fingerprint substrate bands as well as induced conformational changes in phosphatase enzymes’ secondary structure were further examined. Eventually albumin interaction experiments were performed in order to derive their bioavailability.
format Articulo
Preprint
author Parente, Juliana Elena
Naso, Luciana
Jori, Khalil
Franca, Carlos Alberto
da Costa Ferreira, Ana María
Williams, Patricia Ana María
Ferrer, Evelina Gloria
author_facet Parente, Juliana Elena
Naso, Luciana
Jori, Khalil
Franca, Carlos Alberto
da Costa Ferreira, Ana María
Williams, Patricia Ana María
Ferrer, Evelina Gloria
author_sort Parente, Juliana Elena
title In vitro experiments and infrared spectroscopy analysis of acid and alkaline phosphatase inhibition by vanadium complexes
title_short In vitro experiments and infrared spectroscopy analysis of acid and alkaline phosphatase inhibition by vanadium complexes
title_full In vitro experiments and infrared spectroscopy analysis of acid and alkaline phosphatase inhibition by vanadium complexes
title_fullStr In vitro experiments and infrared spectroscopy analysis of acid and alkaline phosphatase inhibition by vanadium complexes
title_full_unstemmed In vitro experiments and infrared spectroscopy analysis of acid and alkaline phosphatase inhibition by vanadium complexes
title_sort in vitro experiments and infrared spectroscopy analysis of acid and alkaline phosphatase inhibition by vanadium complexes
publishDate 2019
url http://sedici.unlp.edu.ar/handle/10915/127267
https://pubs.rsc.org/en/content/articlelanding/2019/NJ/C9NJ01638D
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bdutipo_str Repositorios
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