Adsorption and exchangeability of fibronectin and serum albumin protein corona on annealed polyelectrolyte multilayers and their consequences on cell adhesion

Polyelectrolyte multilayers (PEMs) based on biopolyelectrolytes are highly appealing for the surface engineering of biomaterials and the tuning of cell response and phenotypes for biomedical applications. However, cell adhesion is limited on biopolyelectrolyte PEMs. Thermal annealing provides a simp...

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Detalles Bibliográficos
Autores principales: Muzzio, Nicolás Eduardo, Pasquale, Miguel Ángel, Rios, Xabier, Azzaroni, Omar, Llop, Jordi, Moya, Sergio
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2019
Materias:
Química
Física
Polyelectrolyte multilayer
Thermal annealing
Protein adsorption
Cell adhesion modulation
Fibronectin
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/127239
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-127239
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Física
Polyelectrolyte multilayer
Thermal annealing
Protein adsorption
Cell adhesion modulation
Fibronectin
spellingShingle Química
Física
Polyelectrolyte multilayer
Thermal annealing
Protein adsorption
Cell adhesion modulation
Fibronectin
Muzzio, Nicolás Eduardo
Pasquale, Miguel Ángel
Rios, Xabier
Azzaroni, Omar
Llop, Jordi
Moya, Sergio
Adsorption and exchangeability of fibronectin and serum albumin protein corona on annealed polyelectrolyte multilayers and their consequences on cell adhesion
topic_facet Química
Física
Polyelectrolyte multilayer
Thermal annealing
Protein adsorption
Cell adhesion modulation
Fibronectin
description Polyelectrolyte multilayers (PEMs) based on biopolyelectrolytes are highly appealing for the surface engineering of biomaterials and the tuning of cell response and phenotypes for biomedical applications. However, cell adhesion is limited on biopolyelectrolyte PEMs. Thermal annealing provides a simple means to increase or decrease cell adhesion on PEMs. The work presented here aims to understand cellular interactions with annealed PEMs based on the adsorption and exchangeability of two model proteins: fibronectin (FN), an adhesion protein, and bovine serum albumin (BSA), a nonadhesion protein. Protein adsorption and exchangeability are studied on annealed poly-l-lysine (PLL)/sodium alginate (Alg) and chitosan (Chi)/hyaluronic acid (HA) PEMs using [131I] radiolabeled proteins and gamma counting. Upon annealing cell adhesion is enhanced on PLL/Alg multilayers and decreased on Chi/HA multilayers. For PLL/Alg PEMs, annealing increases adsorption of both FN and BSA and reduces exchangeability. For Chi/HA multilayers, annealing increases BSA adsorption but decreases FN deposition, accompanied by a greater exchangeability. Changes in topographic features of deposited proteins on annealed PLL/Alg hint on changes in the 3D structure of the proteins. Circular dichroism shows that FN retains a large β-sheet contribution upon adsorption to both annealed and unannealed PLL/Alg PEMs, also suggesting changes in tertiary structure.
format Articulo
Preprint
author Muzzio, Nicolás Eduardo
Pasquale, Miguel Ángel
Rios, Xabier
Azzaroni, Omar
Llop, Jordi
Moya, Sergio
author_facet Muzzio, Nicolás Eduardo
Pasquale, Miguel Ángel
Rios, Xabier
Azzaroni, Omar
Llop, Jordi
Moya, Sergio
author_sort Muzzio, Nicolás Eduardo
title Adsorption and exchangeability of fibronectin and serum albumin protein corona on annealed polyelectrolyte multilayers and their consequences on cell adhesion
title_short Adsorption and exchangeability of fibronectin and serum albumin protein corona on annealed polyelectrolyte multilayers and their consequences on cell adhesion
title_full Adsorption and exchangeability of fibronectin and serum albumin protein corona on annealed polyelectrolyte multilayers and their consequences on cell adhesion
title_fullStr Adsorption and exchangeability of fibronectin and serum albumin protein corona on annealed polyelectrolyte multilayers and their consequences on cell adhesion
title_full_unstemmed Adsorption and exchangeability of fibronectin and serum albumin protein corona on annealed polyelectrolyte multilayers and their consequences on cell adhesion
title_sort adsorption and exchangeability of fibronectin and serum albumin protein corona on annealed polyelectrolyte multilayers and their consequences on cell adhesion
publishDate 2019
url http://sedici.unlp.edu.ar/handle/10915/127239
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AT pasqualemiguelangel adsorptionandexchangeabilityoffibronectinandserumalbuminproteincoronaonannealedpolyelectrolytemultilayersandtheirconsequencesoncelladhesion
AT riosxabier adsorptionandexchangeabilityoffibronectinandserumalbuminproteincoronaonannealedpolyelectrolytemultilayersandtheirconsequencesoncelladhesion
AT azzaroniomar adsorptionandexchangeabilityoffibronectinandserumalbuminproteincoronaonannealedpolyelectrolytemultilayersandtheirconsequencesoncelladhesion
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AT moyasergio adsorptionandexchangeabilityoffibronectinandserumalbuminproteincoronaonannealedpolyelectrolytemultilayersandtheirconsequencesoncelladhesion
bdutipo_str Repositorios
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