Calcium chloride treatment modifies cell wall metabolism and activates defense responses in strawberry fruit (Fragaria × ananassa, Duch)

Background Fruit dips in calcium ions solutions have been shown as an effective treatment to extend strawberries (Fragaria × ananassa, Duch) quality during storage. In the present work, strawberry fruit were treated with 10 g L-1 calcium chloride solution and treatment effects on cell wall enzymes a...

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Autores principales: Langer, Silvia Estefanía, Marina, María, Burgos, José Luis, Martínez, Gustavo Adolfo, Civello, Pedro Marcos, Villarreal, Natalia Marina
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2019
Materias:
Química
Calcium chloride
Strawberry
Cell wall metabolism
Pectins
Fruit defense
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/127181
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-127181
record_format dspace
spelling I19-R120-10915-1271812023-07-12T17:47:27Z http://sedici.unlp.edu.ar/handle/10915/127181 issn:1097-0010 issn:0022-5142 Calcium chloride treatment modifies cell wall metabolism and activates defense responses in strawberry fruit (Fragaria × ananassa, Duch) Langer, Silvia Estefanía Marina, María Burgos, José Luis Martínez, Gustavo Adolfo Civello, Pedro Marcos Villarreal, Natalia Marina 2019 2021-10-25T14:06:30Z en Química Calcium chloride Strawberry Cell wall metabolism Pectins Fruit defense Background Fruit dips in calcium ions solutions have been shown as an effective treatment to extend strawberries (Fragaria × ananassa, Duch) quality during storage. In the present work, strawberry fruit were treated with 10 g L-1 calcium chloride solution and treatment effects on cell wall enzymes activities and the expression of encoding genes, as well as enzymes involved in fruit defense responses were investigated. Results Calcium treatment enhanced pectin methylesterase activity while inhibited those corresponding to pectin hydrolases as polygalacturonase and β-galactosidase. The expression of key genes for strawberry pectin metabolism was up-regulated (for FaPME1) and down-regulated (for FaPG1, FaPLB, FaPLC, FaβGal1 and FaAra1) by calcium dips. In agreement, a higher firmness level and ionically-bound pectins (IBPs) amount were detected in calcium-treated fruit compared with controls. The in vitro and in vivo growth rate of fungal pathogen Botrytis cinerea was limited by calcium treatment. Moreover, the activities of polyphenol oxidases, chitinases, peroxidases and β-1,3-glucanases were enhanced by calcium ion dips. Conclusion News insights concerning the biochemical and molecular basis of cell wall preservation and resistance to fungal pathogens on calcium-treated strawberries are provided. Instituto de Fisiología Vegetal Articulo Preprint http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf 4003-4010
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Calcium chloride
Strawberry
Cell wall metabolism
Pectins
Fruit defense
spellingShingle Química
Calcium chloride
Strawberry
Cell wall metabolism
Pectins
Fruit defense
Langer, Silvia Estefanía
Marina, María
Burgos, José Luis
Martínez, Gustavo Adolfo
Civello, Pedro Marcos
Villarreal, Natalia Marina
Calcium chloride treatment modifies cell wall metabolism and activates defense responses in strawberry fruit (Fragaria × ananassa, Duch)
topic_facet Química
Calcium chloride
Strawberry
Cell wall metabolism
Pectins
Fruit defense
description Background Fruit dips in calcium ions solutions have been shown as an effective treatment to extend strawberries (Fragaria × ananassa, Duch) quality during storage. In the present work, strawberry fruit were treated with 10 g L-1 calcium chloride solution and treatment effects on cell wall enzymes activities and the expression of encoding genes, as well as enzymes involved in fruit defense responses were investigated. Results Calcium treatment enhanced pectin methylesterase activity while inhibited those corresponding to pectin hydrolases as polygalacturonase and β-galactosidase. The expression of key genes for strawberry pectin metabolism was up-regulated (for FaPME1) and down-regulated (for FaPG1, FaPLB, FaPLC, FaβGal1 and FaAra1) by calcium dips. In agreement, a higher firmness level and ionically-bound pectins (IBPs) amount were detected in calcium-treated fruit compared with controls. The in vitro and in vivo growth rate of fungal pathogen Botrytis cinerea was limited by calcium treatment. Moreover, the activities of polyphenol oxidases, chitinases, peroxidases and β-1,3-glucanases were enhanced by calcium ion dips. Conclusion News insights concerning the biochemical and molecular basis of cell wall preservation and resistance to fungal pathogens on calcium-treated strawberries are provided.
format Articulo
Preprint
author Langer, Silvia Estefanía
Marina, María
Burgos, José Luis
Martínez, Gustavo Adolfo
Civello, Pedro Marcos
Villarreal, Natalia Marina
author_facet Langer, Silvia Estefanía
Marina, María
Burgos, José Luis
Martínez, Gustavo Adolfo
Civello, Pedro Marcos
Villarreal, Natalia Marina
author_sort Langer, Silvia Estefanía
title Calcium chloride treatment modifies cell wall metabolism and activates defense responses in strawberry fruit (Fragaria × ananassa, Duch)
title_short Calcium chloride treatment modifies cell wall metabolism and activates defense responses in strawberry fruit (Fragaria × ananassa, Duch)
title_full Calcium chloride treatment modifies cell wall metabolism and activates defense responses in strawberry fruit (Fragaria × ananassa, Duch)
title_fullStr Calcium chloride treatment modifies cell wall metabolism and activates defense responses in strawberry fruit (Fragaria × ananassa, Duch)
title_full_unstemmed Calcium chloride treatment modifies cell wall metabolism and activates defense responses in strawberry fruit (Fragaria × ananassa, Duch)
title_sort calcium chloride treatment modifies cell wall metabolism and activates defense responses in strawberry fruit (fragaria × ananassa, duch)
publishDate 2019
url http://sedici.unlp.edu.ar/handle/10915/127181
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