Characterizing monoclonal antibody epitopes by filtered gene fragment phage display

In the present paper, we describe a novel approach to map monoclonal antibody epitopes, using three new monoclonal antibodies that recognize h-TG2 (human transglutaminase 2) as an example. The target gene was fragmented and cloned upstream of an antibiotic-resistance gene, in the vector pPAO2, to se...

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Detalles Bibliográficos
Autores principales: Di Niro, Roberto, Ferrara, Fortunato, Not, Tarcisio, Bradbury, Andrew, Chirdo, Fernando Gabriel, Marzari, Roberto, Sblattero, Daniele
Formato: Articulo
Lenguaje:Inglés
Publicado: 2005
Materias:
Bioquímica
Phage display
Epitope mapping
β-lactamase
Transglutaminase
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/126484
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-126484
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Bioquímica
Phage display
Epitope mapping
β-lactamase
Transglutaminase
spellingShingle Bioquímica
Phage display
Epitope mapping
β-lactamase
Transglutaminase
Di Niro, Roberto
Ferrara, Fortunato
Not, Tarcisio
Bradbury, Andrew
Chirdo, Fernando Gabriel
Marzari, Roberto
Sblattero, Daniele
Characterizing monoclonal antibody epitopes by filtered gene fragment phage display
topic_facet Bioquímica
Phage display
Epitope mapping
β-lactamase
Transglutaminase
description In the present paper, we describe a novel approach to map monoclonal antibody epitopes, using three new monoclonal antibodies that recognize h-TG2 (human transglutaminase 2) as an example. The target gene was fragmented and cloned upstream of an antibiotic-resistance gene, in the vector pPAO2, to select for in-frame polypeptides. After removal of the antibiotic-resistance gene by Cre/Lox recombination, an antigen fragment phage display library was created and selected against specific monoclonal antibodies. Using the h-TG2 fragment library, we were able to identify epitopes. This technique can also be broadly applied to the study of protein-protein interactions.
format Articulo
Articulo
author Di Niro, Roberto
Ferrara, Fortunato
Not, Tarcisio
Bradbury, Andrew
Chirdo, Fernando Gabriel
Marzari, Roberto
Sblattero, Daniele
author_facet Di Niro, Roberto
Ferrara, Fortunato
Not, Tarcisio
Bradbury, Andrew
Chirdo, Fernando Gabriel
Marzari, Roberto
Sblattero, Daniele
author_sort Di Niro, Roberto
title Characterizing monoclonal antibody epitopes by filtered gene fragment phage display
title_short Characterizing monoclonal antibody epitopes by filtered gene fragment phage display
title_full Characterizing monoclonal antibody epitopes by filtered gene fragment phage display
title_fullStr Characterizing monoclonal antibody epitopes by filtered gene fragment phage display
title_full_unstemmed Characterizing monoclonal antibody epitopes by filtered gene fragment phage display
title_sort characterizing monoclonal antibody epitopes by filtered gene fragment phage display
publishDate 2005
url http://sedici.unlp.edu.ar/handle/10915/126484
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