Humanized celiac-prone epithelium in vitro express MHC-II and co-stimulatory molecules necessary for gluten peptide presentation
Background: The role intestinal epithelial cells (IECs) play in the breakdown of tolerance to gluten at an early stage in celiac disease (CeD) is unclear. Epithelial stress is a feature of CeD, and although the triggers are largely unknown, it is accompanied by expression of several markers that...
Guardado en:
| Autores principales: | , , , , , |
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| Formato: | Articulo Comunicacion |
| Lenguaje: | Inglés |
| Publicado: |
2020
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/126417 |
| Aporte de: |
| Sumario: | Background: The role intestinal epithelial cells (IECs) play in the breakdown of
tolerance to gluten at an early stage in celiac disease (CeD) is unclear. Epithelial stress
is a feature of CeD, and although the triggers are largely unknown, it is accompanied
by expression of several markers that could be involved in initiation of inflammatory
responses. IECs have been shown to express MHC class II (MHC-II) molecules and
participate in antigen presentation in several models. Whether IECs can participate in
gluten peptide presentation, the major environmental trigger in celiac disease, is
unknown. To study this, a model expressing human MHC-II, HLA DQ8 or HLADQ2, would be required. Aims: To develop organoid monolayers from transgenic mice expressing human
celiac risk genes: HLA-DQ8 and -DQ2. To investigate conditions leading to the
induction of epithelial MHC-II and its main co-stimulatory molecules, CD80, CD86
and CD40, that could enable early gluten peptide presentation. |
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