Humanized celiac-prone epithelium in vitro express MHC-II and co-stimulatory molecules necessary for gluten peptide presentation

Background: The role intestinal epithelial cells (IECs) play in the breakdown of tolerance to gluten at an early stage in celiac disease (CeD) is unclear. Epithelial stress is a feature of CeD, and although the triggers are largely unknown, it is accompanied by expression of several markers that...

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Autores principales: Rahmani, Sara, Galipeau, Heather J., Su, Hsuan-Ming, Chirdo, Fernando Gabriel, Didar, Tohid F., Verdu, Elena F.
Formato: Articulo Comunicacion
Lenguaje:Inglés
Publicado: 2020
Materias:
Ciencias Exactas
intestinal epithelial cells
celiac disease
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/126417
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-126417
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
intestinal epithelial cells
celiac disease
spellingShingle Ciencias Exactas
intestinal epithelial cells
celiac disease
Rahmani, Sara
Galipeau, Heather J.
Su, Hsuan-Ming
Chirdo, Fernando Gabriel
Didar, Tohid F.
Verdu, Elena F.
Humanized celiac-prone epithelium in vitro express MHC-II and co-stimulatory molecules necessary for gluten peptide presentation
topic_facet Ciencias Exactas
intestinal epithelial cells
celiac disease
description Background: The role intestinal epithelial cells (IECs) play in the breakdown of tolerance to gluten at an early stage in celiac disease (CeD) is unclear. Epithelial stress is a feature of CeD, and although the triggers are largely unknown, it is accompanied by expression of several markers that could be involved in initiation of inflammatory responses. IECs have been shown to express MHC class II (MHC-II) molecules and participate in antigen presentation in several models. Whether IECs can participate in gluten peptide presentation, the major environmental trigger in celiac disease, is unknown. To study this, a model expressing human MHC-II, HLA DQ8 or HLADQ2, would be required. Aims: To develop organoid monolayers from transgenic mice expressing human celiac risk genes: HLA-DQ8 and -DQ2. To investigate conditions leading to the induction of epithelial MHC-II and its main co-stimulatory molecules, CD80, CD86 and CD40, that could enable early gluten peptide presentation.
format Articulo
Comunicacion
author Rahmani, Sara
Galipeau, Heather J.
Su, Hsuan-Ming
Chirdo, Fernando Gabriel
Didar, Tohid F.
Verdu, Elena F.
author_facet Rahmani, Sara
Galipeau, Heather J.
Su, Hsuan-Ming
Chirdo, Fernando Gabriel
Didar, Tohid F.
Verdu, Elena F.
author_sort Rahmani, Sara
title Humanized celiac-prone epithelium in vitro express MHC-II and co-stimulatory molecules necessary for gluten peptide presentation
title_short Humanized celiac-prone epithelium in vitro express MHC-II and co-stimulatory molecules necessary for gluten peptide presentation
title_full Humanized celiac-prone epithelium in vitro express MHC-II and co-stimulatory molecules necessary for gluten peptide presentation
title_fullStr Humanized celiac-prone epithelium in vitro express MHC-II and co-stimulatory molecules necessary for gluten peptide presentation
title_full_unstemmed Humanized celiac-prone epithelium in vitro express MHC-II and co-stimulatory molecules necessary for gluten peptide presentation
title_sort humanized celiac-prone epithelium in vitro express mhc-ii and co-stimulatory molecules necessary for gluten peptide presentation
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/126417
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