New forms of induction of apoptosis in aggressive lymphoma using peptides that interrupt the RAS / RAF interaction

RAS-RAF-MEK-ERK is a key pathway for apoptosis regulation in cancer cells. B-Raf-inhibitors such as PLX4032 peptide was developed by Institute Curie-Université Pierre et Marie Curie in order to induce apoptosis in cancer cells. Objective: To demonstrate pro-apoptotic properties and survival outcome...

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Detalles Bibliográficos
Autores principales: Bergna, Cecilia, Marín, Gustavo Horacio, Maiz, Mercedes Guadalupe, Bruzzoni Giovanelli, H., Ponzinibbio, Carlos, Schinella, Guillermo Raúl, Errecalde, Jorge Oscar, Rebollo, Angelita
Formato: Articulo
Lenguaje:Inglés
Publicado: 2019
Materias:
Medicina
T-LYMPHOMA
NHL-T
Peptides
Apoptosis
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/126192
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-126192
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Medicina
T-LYMPHOMA
NHL-T
Peptides
Apoptosis
spellingShingle Medicina
T-LYMPHOMA
NHL-T
Peptides
Apoptosis
Bergna, Cecilia
Marín, Gustavo Horacio
Maiz, Mercedes Guadalupe
Bruzzoni Giovanelli, H.
Ponzinibbio, Carlos
Schinella, Guillermo Raúl
Errecalde, Jorge Oscar
Rebollo, Angelita
New forms of induction of apoptosis in aggressive lymphoma using peptides that interrupt the RAS / RAF interaction
topic_facet Medicina
T-LYMPHOMA
NHL-T
Peptides
Apoptosis
description RAS-RAF-MEK-ERK is a key pathway for apoptosis regulation in cancer cells. B-Raf-inhibitors such as PLX4032 peptide was developed by Institute Curie-Université Pierre et Marie Curie in order to induce apoptosis in cancer cells. Objective: To demonstrate pro-apoptotic properties and survival outcome of EP2014/064243 peptide in murine aggressive lymphoma. Material and methods: BALBc mice with T-lymphoma were randomized assigned either in Group A (peptide+cyclophosphamide-CFM); Group B (peptides), Group C (CFM-control) or Control D (Cl-Na 0.9%-SF control group). Survival probability was calculated by Kaplan-Meier analysis. Apoptosis was detected using TUNEL technique. The protocol was approved by the Institutional Committee for Animal Care (CICUAL: T04-01-2015). Results: The median survival was 24 days (21.6-26.4) for placebo, 33 days (28.0-35.4) for the CFM monotherapy group, 33 (27.1-35.8) for the peptide group and 34 days (24,4-40) for CFM-peptide combined treatment (p < 0.05). In lymph node tissue the mean TUNEL positive cells per field for each treatment group was 2, 12 and 13 and 35 for SF, CFM, peptide and combined therapy (p < 0.05). Conclusion: These findings suggest that in murine aggressive lymphoma treated by an experimental peptide in addition with CFM, had an exponentially pro-apoptotic effect than CFM alone, suggesting that the peptide potentiated the anti-tumoural effect of CFM.
format Articulo
Articulo
author Bergna, Cecilia
Marín, Gustavo Horacio
Maiz, Mercedes Guadalupe
Bruzzoni Giovanelli, H.
Ponzinibbio, Carlos
Schinella, Guillermo Raúl
Errecalde, Jorge Oscar
Rebollo, Angelita
author_facet Bergna, Cecilia
Marín, Gustavo Horacio
Maiz, Mercedes Guadalupe
Bruzzoni Giovanelli, H.
Ponzinibbio, Carlos
Schinella, Guillermo Raúl
Errecalde, Jorge Oscar
Rebollo, Angelita
author_sort Bergna, Cecilia
title New forms of induction of apoptosis in aggressive lymphoma using peptides that interrupt the RAS / RAF interaction
title_short New forms of induction of apoptosis in aggressive lymphoma using peptides that interrupt the RAS / RAF interaction
title_full New forms of induction of apoptosis in aggressive lymphoma using peptides that interrupt the RAS / RAF interaction
title_fullStr New forms of induction of apoptosis in aggressive lymphoma using peptides that interrupt the RAS / RAF interaction
title_full_unstemmed New forms of induction of apoptosis in aggressive lymphoma using peptides that interrupt the RAS / RAF interaction
title_sort new forms of induction of apoptosis in aggressive lymphoma using peptides that interrupt the ras / raf interaction
publishDate 2019
url http://sedici.unlp.edu.ar/handle/10915/126192
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