Seven new microcystin variants discovered from a native <i>Microcystis aeruginosa</i> strain – unambiguous assignment of product ions by tandem mass spectrometry

RATIONALE: High-resolution mass spectrometry was applied to the study of a Microcystis aeruginosa strain previously reported as a [D-Leu1]MC-LR producer. Detailed analysis revealed new microcystin (MC) variants produced from the strain, and seven of these were previously unreported variants. This wo...

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Autores principales: Qi, Yulin, Rosso, Lorena, Sedan, Daniela Yazmine, Giannuzzi, Leda, Andrinolo, Darío, Volmer, Dietrich A.
Formato: Articulo
Lenguaje:Español
Publicado: 2015
Materias:
Biología
Microcistinas
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/125879
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-125879
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Español
topic Biología
Microcistinas
spellingShingle Biología
Microcistinas
Qi, Yulin
Rosso, Lorena
Sedan, Daniela Yazmine
Giannuzzi, Leda
Andrinolo, Darío
Volmer, Dietrich A.
Seven new microcystin variants discovered from a native <i>Microcystis aeruginosa</i> strain – unambiguous assignment of product ions by tandem mass spectrometry
topic_facet Biología
Microcistinas
description RATIONALE: High-resolution mass spectrometry was applied to the study of a Microcystis aeruginosa strain previously reported as a [D-Leu1]MC-LR producer. Detailed analysis revealed new microcystin (MC) variants produced from the strain, and seven of these were previously unreported variants. This work shows the importance of mass accuracy for the identification of unknown MCs. METHODS: The M. aeruginosa strain was isolated from a bloom sample collected from Argentina and acclimated to lab conditions. The MC variants in the strain were separated by UV/Vis detection-guided high-performance liquid chromatography, and their structures were unambiguous determined by tandem mass spectrometry (MS/MS). RESULTS: A simple strategywas developed for quickly locating the low-abundanceMC precursors fromcomplex samples. MS/MS anlysis revealed ten MC variants produced from the strain, of which seven have never been reported before. CONCLUSIONS: This work shows the interference of isobarics and isomers in the study of unknown MCs, and, therefore, high mass accuracy is important to avoid false assignments. Moreover, the peak list provided here (30–50 fragments unambiguously assigned for ten MCs) can be used as a reference for the discovery of MCs from environmental samples.
format Articulo
Articulo
author Qi, Yulin
Rosso, Lorena
Sedan, Daniela Yazmine
Giannuzzi, Leda
Andrinolo, Darío
Volmer, Dietrich A.
author_facet Qi, Yulin
Rosso, Lorena
Sedan, Daniela Yazmine
Giannuzzi, Leda
Andrinolo, Darío
Volmer, Dietrich A.
author_sort Qi, Yulin
title Seven new microcystin variants discovered from a native <i>Microcystis aeruginosa</i> strain – unambiguous assignment of product ions by tandem mass spectrometry
title_short Seven new microcystin variants discovered from a native <i>Microcystis aeruginosa</i> strain – unambiguous assignment of product ions by tandem mass spectrometry
title_full Seven new microcystin variants discovered from a native <i>Microcystis aeruginosa</i> strain – unambiguous assignment of product ions by tandem mass spectrometry
title_fullStr Seven new microcystin variants discovered from a native <i>Microcystis aeruginosa</i> strain – unambiguous assignment of product ions by tandem mass spectrometry
title_full_unstemmed Seven new microcystin variants discovered from a native <i>Microcystis aeruginosa</i> strain – unambiguous assignment of product ions by tandem mass spectrometry
title_sort seven new microcystin variants discovered from a native <i>microcystis aeruginosa</i> strain – unambiguous assignment of product ions by tandem mass spectrometry
publishDate 2015
url http://sedici.unlp.edu.ar/handle/10915/125879
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