Toll-Like Receptor 4 and NLRP3 Caspase 1- Interleukin-1β-Axis are Not Involved in Colon Ascendens Stent Peritonitis-Associated Heart Disease

Hemodynamic collapse and myocardial dysfunction are among the major causes of death in severe sepsis. The purpose of this study was to assess the role played by toll-like receptor 4 and by the NLRP3 inflammasome in the cardiac dysfunction that occurs after high-grade polymicrobial sepsis. We perform...

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Autores principales: Lopez Alarcon, Micaela, Fernández Ruocco, Julieta, Ferreira, Fabiano, Paula-Neto, Heitor A., Sepúlveda, Marisa Noemí, Vila Petroff, Martín Gerardo, Bastos Carvalho, Adriana, Peroba Ramos, Isalira, Branda, Hugo Justino, Paiva, Claudia N., Medei, Emiliano
Formato: Articulo
Lenguaje:Inglés
Publicado: 2018
Materias:
Ciencias Médicas
Cardioimmunology
Electrophysiology
Interleukin-1b
Innate immunology
Sepsis
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/125728
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-125728
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Cardioimmunology
Electrophysiology
Interleukin-1b
Innate immunology
Sepsis
spellingShingle Ciencias Médicas
Cardioimmunology
Electrophysiology
Interleukin-1b
Innate immunology
Sepsis
Lopez Alarcon, Micaela
Fernández Ruocco, Julieta
Ferreira, Fabiano
Paula-Neto, Heitor A.
Sepúlveda, Marisa Noemí
Vila Petroff, Martín Gerardo
Bastos Carvalho, Adriana
Peroba Ramos, Isalira
Branda, Hugo Justino
Paiva, Claudia N.
Medei, Emiliano
Toll-Like Receptor 4 and NLRP3 Caspase 1- Interleukin-1β-Axis are Not Involved in Colon Ascendens Stent Peritonitis-Associated Heart Disease
topic_facet Ciencias Médicas
Cardioimmunology
Electrophysiology
Interleukin-1b
Innate immunology
Sepsis
description Hemodynamic collapse and myocardial dysfunction are among the major causes of death in severe sepsis. The purpose of this study was to assess the role played by toll-like receptor 4 and by the NLRP3 inflammasome in the cardiac dysfunction that occurs after high-grade polymicrobial sepsis. We performed the colon ascendens stent peritonitis (CASP) surgery in Tlr4, Nlrp3, and caspase-1 mice. We also assessed for the first time the electrical heart function in the colon ascendens stent peritonitis (CASP) model. The QJ interval was increased in wild-type C57BL/6J mice after CASP when compared with sham controls, a result paralleled by an increase in the cardiac action potential (AP) duration (APD). The decreases in ejection fraction (EF), left ventricle end diastolic volume, stroke volume, and cardiac output found after CASP were similar among all groups of mice. Similar heart response was found when Nlrp3 mice were submitted to high-grade cecal ligation and puncture. Despite developing cardiac dysfunction similar to wild types after CASP, Nlrp3 mice had reduced circulating levels of interleukin (IL)-1β, IL-6 and tumor necrosis factor-α. Our results demonstrate that the genetic ablation of Tlr4, Nlrp3, and caspase-1 does not prevent the cardiac dysfunction, despite preventing the increase in pro-inflammatory cytokines, indicating that these are not feasible targets to therapy in high-grade sepsis.
format Articulo
Articulo
author Lopez Alarcon, Micaela
Fernández Ruocco, Julieta
Ferreira, Fabiano
Paula-Neto, Heitor A.
Sepúlveda, Marisa Noemí
Vila Petroff, Martín Gerardo
Bastos Carvalho, Adriana
Peroba Ramos, Isalira
Branda, Hugo Justino
Paiva, Claudia N.
Medei, Emiliano
author_facet Lopez Alarcon, Micaela
Fernández Ruocco, Julieta
Ferreira, Fabiano
Paula-Neto, Heitor A.
Sepúlveda, Marisa Noemí
Vila Petroff, Martín Gerardo
Bastos Carvalho, Adriana
Peroba Ramos, Isalira
Branda, Hugo Justino
Paiva, Claudia N.
Medei, Emiliano
author_sort Lopez Alarcon, Micaela
title Toll-Like Receptor 4 and NLRP3 Caspase 1- Interleukin-1β-Axis are Not Involved in Colon Ascendens Stent Peritonitis-Associated Heart Disease
title_short Toll-Like Receptor 4 and NLRP3 Caspase 1- Interleukin-1β-Axis are Not Involved in Colon Ascendens Stent Peritonitis-Associated Heart Disease
title_full Toll-Like Receptor 4 and NLRP3 Caspase 1- Interleukin-1β-Axis are Not Involved in Colon Ascendens Stent Peritonitis-Associated Heart Disease
title_fullStr Toll-Like Receptor 4 and NLRP3 Caspase 1- Interleukin-1β-Axis are Not Involved in Colon Ascendens Stent Peritonitis-Associated Heart Disease
title_full_unstemmed Toll-Like Receptor 4 and NLRP3 Caspase 1- Interleukin-1β-Axis are Not Involved in Colon Ascendens Stent Peritonitis-Associated Heart Disease
title_sort toll-like receptor 4 and nlrp3 caspase 1- interleukin-1β-axis are not involved in colon ascendens stent peritonitis-associated heart disease
publishDate 2018
url http://sedici.unlp.edu.ar/handle/10915/125728
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