Nanomotion Spectroscopy as a New Approach to Characterize Bacterial Virulence

Atomic force microscopy (AFM)-based nanomotion detection is a label-free technique that has been used to monitor the response of microorganisms to antibiotics in a time frame of minutes. The method consists of attaching living organisms onto an AFM cantilever and in monitoring its nanometric scale o...

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Detalles Bibliográficos
Autores principales:	Villalba, María Inés, Venturelli, Leonardo, Willaert, Ronnie, Vela, María Elena, Yantorno, Osvaldo Miguel, Dietler, Giovanni, Longo, Giovanni, Kasas, Sandor
Formato:	Articulo Comunicacion
Lenguaje:	Inglés
Publicado:	2021
Materias:	Ciencias Exactas Química nanomotion bacteria AFM B. pertussis
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/125426 https://www.mdpi.com/2076-2607/9/8/1545
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-125426
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Exactas Química nanomotion bacteria AFM B. pertussis
spellingShingle	Ciencias Exactas Química nanomotion bacteria AFM B. pertussis Villalba, María Inés Venturelli, Leonardo Willaert, Ronnie Vela, María Elena Yantorno, Osvaldo Miguel Dietler, Giovanni Longo, Giovanni Kasas, Sandor Nanomotion Spectroscopy as a New Approach to Characterize Bacterial Virulence
topic_facet	Ciencias Exactas Química nanomotion bacteria AFM B. pertussis
description	Atomic force microscopy (AFM)-based nanomotion detection is a label-free technique that has been used to monitor the response of microorganisms to antibiotics in a time frame of minutes. The method consists of attaching living organisms onto an AFM cantilever and in monitoring its nanometric scale oscillations as a function of different physical-chemical stimuli. Up to now, we only used the cantilever oscillations variance signal to assess the viability of the attached organisms. In this contribution, we demonstrate that a more precise analysis of the motion pattern of the cantilever can unveil relevant medical information about bacterial phenotype. We used B. pertussis as the model organism, it is a slowly growing Gram-negative bacteria which is the agent of whooping cough. It was previously demonstrated that B. pertussis can expresses different phenotypes as a function of the physical-chemical properties of the environment. In this contribution, we highlight that B. pertussis generates a cantilever movement pattern that depends on its phenotype. More precisely, we noticed that nanometric scale oscillations of B. pertussis can be correlated with the virulence state of the bacteria. The results indicate a correlation between metabolic/virulent bacterial states and bacterial nanomotion pattern and paves the way to novel rapid and label-free pathogenic microorganism detection assays.
format	Articulo Comunicacion
author	Villalba, María Inés Venturelli, Leonardo Willaert, Ronnie Vela, María Elena Yantorno, Osvaldo Miguel Dietler, Giovanni Longo, Giovanni Kasas, Sandor
author_facet	Villalba, María Inés Venturelli, Leonardo Willaert, Ronnie Vela, María Elena Yantorno, Osvaldo Miguel Dietler, Giovanni Longo, Giovanni Kasas, Sandor
author_sort	Villalba, María Inés
title	Nanomotion Spectroscopy as a New Approach to Characterize Bacterial Virulence
title_short	Nanomotion Spectroscopy as a New Approach to Characterize Bacterial Virulence
title_full	Nanomotion Spectroscopy as a New Approach to Characterize Bacterial Virulence
title_fullStr	Nanomotion Spectroscopy as a New Approach to Characterize Bacterial Virulence
title_full_unstemmed	Nanomotion Spectroscopy as a New Approach to Characterize Bacterial Virulence
title_sort	nanomotion spectroscopy as a new approach to characterize bacterial virulence
publishDate	2021
url	http://sedici.unlp.edu.ar/handle/10915/125426 https://www.mdpi.com/2076-2607/9/8/1545
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bdutipo_str	Repositorios
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Nanomotion Spectroscopy as a New Approach to Characterize Bacterial Virulence

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