Efficiency of Antimicrobial Photodynamic Therapy with Photodithazine® on MSSA and MRSA Strains

Staphylococccus aureus is a ubiquitous and opportunistic bacteria associated with high mortality rates. Antimicrobial photodynamic therapy (aPDT) is based on the application of a light source and a photosensitizer that can interact with molecular oxygen, forming Reactive Oxygen Species (ROS) that re...

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Detalles Bibliográficos
Autores principales:	Müller Nunes Souza, Beatriz, Guerra Pinto, Juliana, Correia Pereira, André Henrique, Miñán, Alejandro, Ferreira Strixino, Juliana
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2021
Materias:	Física Química Ciencias Exactas antimicrobial photodynamic therapy MRSA MSSA photodithazine®
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/125422 https://www.mdpi.com/2079-6382/10/7/869
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-125422
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Física Química Ciencias Exactas antimicrobial photodynamic therapy MRSA MSSA photodithazine®
spellingShingle	Física Química Ciencias Exactas antimicrobial photodynamic therapy MRSA MSSA photodithazine® Müller Nunes Souza, Beatriz Guerra Pinto, Juliana Correia Pereira, André Henrique Miñán, Alejandro Ferreira Strixino, Juliana Efficiency of Antimicrobial Photodynamic Therapy with Photodithazine® on MSSA and MRSA Strains
topic_facet	Física Química Ciencias Exactas antimicrobial photodynamic therapy MRSA MSSA photodithazine®
description	Staphylococccus aureus is a ubiquitous and opportunistic bacteria associated with high mortality rates. Antimicrobial photodynamic therapy (aPDT) is based on the application of a light source and a photosensitizer that can interact with molecular oxygen, forming Reactive Oxygen Species (ROS) that result in bacterial inactivation. This study aimed to analyze, in vitro, the action of aPDT with Photodithazine® (PDZ) in methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) strains. The strains were incubated with PDZ at 25, 50, 75, and 100 mg/L for 15 min and irradiated with fluences of 25, 50, and 100 J/cm2. The internalization of PDZ was evaluated by confocal microscopy, the bacterial growth by counting the number of colony-forming units, as well as the bacterial metabolic activity post-aPDT and the production of ROS. In both strains, the photosensitizer was internalized; the production of ROS increased when the aPDT was applied; there was a bacterial reduction compared to the control at all the evaluated fluences and concentrations; and, in most parameters, it was obtained complete inactivation with significant difference (p < 0.05). The implementation of aPDT with PDZ in clinical strains of S. aureus has resulted in its complete inactivation, including the MRSA strains.
format	Articulo Articulo
author	Müller Nunes Souza, Beatriz Guerra Pinto, Juliana Correia Pereira, André Henrique Miñán, Alejandro Ferreira Strixino, Juliana
author_facet	Müller Nunes Souza, Beatriz Guerra Pinto, Juliana Correia Pereira, André Henrique Miñán, Alejandro Ferreira Strixino, Juliana
author_sort	Müller Nunes Souza, Beatriz
title	Efficiency of Antimicrobial Photodynamic Therapy with Photodithazine® on MSSA and MRSA Strains
title_short	Efficiency of Antimicrobial Photodynamic Therapy with Photodithazine® on MSSA and MRSA Strains
title_full	Efficiency of Antimicrobial Photodynamic Therapy with Photodithazine® on MSSA and MRSA Strains
title_fullStr	Efficiency of Antimicrobial Photodynamic Therapy with Photodithazine® on MSSA and MRSA Strains
title_full_unstemmed	Efficiency of Antimicrobial Photodynamic Therapy with Photodithazine® on MSSA and MRSA Strains
title_sort	efficiency of antimicrobial photodynamic therapy with photodithazine® on mssa and mrsa strains
publishDate	2021
url	http://sedici.unlp.edu.ar/handle/10915/125422 https://www.mdpi.com/2079-6382/10/7/869
work_keys_str_mv	AT mullernunessouzabeatriz efficiencyofantimicrobialphotodynamictherapywithphotodithazineonmssaandmrsastrains AT guerrapintojuliana efficiencyofantimicrobialphotodynamictherapywithphotodithazineonmssaandmrsastrains AT correiapereiraandrehenrique efficiencyofantimicrobialphotodynamictherapywithphotodithazineonmssaandmrsastrains AT minanalejandro efficiencyofantimicrobialphotodynamictherapywithphotodithazineonmssaandmrsastrains AT ferreirastrixinojuliana efficiencyofantimicrobialphotodynamictherapywithphotodithazineonmssaandmrsastrains
bdutipo_str	Repositorios
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Efficiency of Antimicrobial Photodynamic Therapy with Photodithazine® on MSSA and MRSA Strains

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