Tomato Wall-Associated Kinase SlWak1 Depends on Fls2/Fls3 to Promote Apoplastic Immune Responses to <i>Pseudomonas syringae</i>

Wall-associated kinases (Waks) are important components of plant immunity against various pathogens, including the bacterium <i>Pseudomonas syringae</i> pv. tomato (Pst). However, the molecular mechanisms of their role(s) in plant immunity are largely unknown. In tomato (<i>Solanum...

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Detalles Bibliográficos
Autores principales: Zhang, Ning, Pombo, Marina Alejandra, Rosli, Hernán Guillermo, Martina, Gregory B.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2020
Materias:
Ciencias Agrarias
Ciencias Naturales
Wall-Associated Kinase
Pseudomonas syringae
Solanum lycopersicum
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/125277
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-125277
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Agrarias
Ciencias Naturales
Wall-Associated Kinase
Pseudomonas syringae
Solanum lycopersicum
spellingShingle Ciencias Agrarias
Ciencias Naturales
Wall-Associated Kinase
Pseudomonas syringae
Solanum lycopersicum
Zhang, Ning
Pombo, Marina Alejandra
Rosli, Hernán Guillermo
Martina, Gregory B.
Tomato Wall-Associated Kinase SlWak1 Depends on Fls2/Fls3 to Promote Apoplastic Immune Responses to <i>Pseudomonas syringae</i>
topic_facet Ciencias Agrarias
Ciencias Naturales
Wall-Associated Kinase
Pseudomonas syringae
Solanum lycopersicum
description Wall-associated kinases (Waks) are important components of plant immunity against various pathogens, including the bacterium <i>Pseudomonas syringae</i> pv. tomato (Pst). However, the molecular mechanisms of their role(s) in plant immunity are largely unknown. In tomato (<i>Solanum lycopersicum</i>), wall-associated kinase 1 (SlWak1), has been implicated in pattern recognition receptor (PRR)-triggered immunity (PTI) because its transcript abundance increases significantly after treatment with the flagellin-derived, microbe-associated molecular patterns flg22 and flgII-28, which activate the PRRs Fls2 and Fls3, respectively. We generated two <i>SlWak1</i> tomato mutants (Δwak1) using CRISPR/Cas9 gene editing technology and investigated the role of <i>SlWak1</i> in tomato–<i>Pst</i> interactions. Late PTI responses activated in the apoplast by flg22 or flgII-28 were compromised in Δwak1 plants, but PTI at the leaf surface was unaffected. The Δwak1 plants developed fewer callose deposits than wild-type plants, but retained early PTI responses such as generation of reactive oxygen species and activation of mitogen-activated protein kinases upon exposure to flg22 and flgII-28. Induction of Wak1 gene expression by flg22 and flgII-28 was greatly reduced in a tomato mutant lacking Fls2 and Fls3, but induction of Fls3 gene expression by flgII-28 was unaffected in Δwak1 plants. After Pst inoculation, Δwak1 plants developed disease symptoms more slowly than Δfls2.1/2.2/3 mutant plants, although ultimately, both plants were similarly susceptible. SlWak1 coimmunoprecipitated with both Fls2 and Fls3, independently of flg22/flgII-28 or of BRASSINOSTEROID INSENSITIVE1-ASSOCIATED RECEPTOR KINASE1. These observations suggest that SlWak1 acts in a complex with Fls2/Fls3 and is important at later stages of PTI in the apoplast.
format Articulo
Articulo
author Zhang, Ning
Pombo, Marina Alejandra
Rosli, Hernán Guillermo
Martina, Gregory B.
author_facet Zhang, Ning
Pombo, Marina Alejandra
Rosli, Hernán Guillermo
Martina, Gregory B.
author_sort Zhang, Ning
title Tomato Wall-Associated Kinase SlWak1 Depends on Fls2/Fls3 to Promote Apoplastic Immune Responses to <i>Pseudomonas syringae</i>
title_short Tomato Wall-Associated Kinase SlWak1 Depends on Fls2/Fls3 to Promote Apoplastic Immune Responses to <i>Pseudomonas syringae</i>
title_full Tomato Wall-Associated Kinase SlWak1 Depends on Fls2/Fls3 to Promote Apoplastic Immune Responses to <i>Pseudomonas syringae</i>
title_fullStr Tomato Wall-Associated Kinase SlWak1 Depends on Fls2/Fls3 to Promote Apoplastic Immune Responses to <i>Pseudomonas syringae</i>
title_full_unstemmed Tomato Wall-Associated Kinase SlWak1 Depends on Fls2/Fls3 to Promote Apoplastic Immune Responses to <i>Pseudomonas syringae</i>
title_sort tomato wall-associated kinase slwak1 depends on fls2/fls3 to promote apoplastic immune responses to <i>pseudomonas syringae</i>
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/125277
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