A dynamic cell recruitment process drives growth of the Drosophila wing by overscaling the Vestigial expression pattern

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Organs mainly attain their size by cell growth and proliferation, but sometimes also grow through recruitment of undifferentiated cells. Here we investigate the participation of cell recruitment in establishing the pattern of Vestigial (Vg), the product of the wing selector gene in Drosophila. We fi...

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Detalles Bibliográficos
Autores principales:	Muñoz Nava, Luis Manuel, Álvarez, Hugo Ariel, Flores Flores, Marycruz, Chara, Osvaldo, Nahmad, Marcos
Formato:	Articulo Preprint
Lenguaje:	Inglés
Publicado:	2019
Materias:	Biología Organ growth Patterning Vestigial Cell recruitment Scaling Drosophila wing disc
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/125213
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-125213
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Biología Organ growth Patterning Vestigial Cell recruitment Scaling Drosophila wing disc
spellingShingle	Biología Organ growth Patterning Vestigial Cell recruitment Scaling Drosophila wing disc Muñoz Nava, Luis Manuel Álvarez, Hugo Ariel Flores Flores, Marycruz Chara, Osvaldo Nahmad, Marcos A dynamic cell recruitment process drives growth of the Drosophila wing by overscaling the Vestigial expression pattern
topic_facet	Biología Organ growth Patterning Vestigial Cell recruitment Scaling Drosophila wing disc
description	Organs mainly attain their size by cell growth and proliferation, but sometimes also grow through recruitment of undifferentiated cells. Here we investigate the participation of cell recruitment in establishing the pattern of Vestigial (Vg), the product of the wing selector gene in Drosophila. We find that the Vg pattern overscales along the dorsal-ventral (DV) axis of the wing imaginal disc, i.e., it expands faster than the DV length of the pouch. This overscaling cannot be explained by differential proliferation or apoptosis, nor correlates with the dynamics of the Wingless gradient, which orchestrates patterning along the DV axis. We show that Vg overscaling is recapitulated by a mathematical model that explicitly considers cell recruitment. Experimentally, when cell recruitment is genetically impaired, the Vg pattern almost perfectly scales and adult wings are 20% smaller, demonstrating that cell recruitment contributes to organ growth. Furthermore, using lineage-tracing tools, we find that cell recruitment takes place in a specific time during normal development. Altogether, our work quantitatively shows when, how, and by how much cell recruitment shapes the Vg pattern and drives growth of the Drosophila wing. Summary statement Cell recruitment enhances growth of the developing Drosophila wing by expanding the expression of the wing selector gene, vestigial.
format	Articulo Preprint
author	Muñoz Nava, Luis Manuel Álvarez, Hugo Ariel Flores Flores, Marycruz Chara, Osvaldo Nahmad, Marcos
author_facet	Muñoz Nava, Luis Manuel Álvarez, Hugo Ariel Flores Flores, Marycruz Chara, Osvaldo Nahmad, Marcos
author_sort	Muñoz Nava, Luis Manuel
title	A dynamic cell recruitment process drives growth of the Drosophila wing by overscaling the Vestigial expression pattern
title_short	A dynamic cell recruitment process drives growth of the Drosophila wing by overscaling the Vestigial expression pattern
title_full	A dynamic cell recruitment process drives growth of the Drosophila wing by overscaling the Vestigial expression pattern
title_fullStr	A dynamic cell recruitment process drives growth of the Drosophila wing by overscaling the Vestigial expression pattern
title_full_unstemmed	A dynamic cell recruitment process drives growth of the Drosophila wing by overscaling the Vestigial expression pattern
title_sort	dynamic cell recruitment process drives growth of the drosophila wing by overscaling the vestigial expression pattern
publishDate	2019
url	http://sedici.unlp.edu.ar/handle/10915/125213
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bdutipo_str	Repositorios
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A dynamic cell recruitment process drives growth of the Drosophila wing by overscaling the Vestigial expression pattern

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