Enhancing laccase production by white-rot fungus Funalia floccosa LPSC 232 in co-culture with Penicillium commune GHAIE86

To obtain enzymatic preparations with higher laccase activity levels from Funalia floccosa LPSC 232, available for use in several applications, co-cultures with six filamentous microfungi were tested. A laccase non-producing soil fungus, identified as Penicillium commune GHAIE86, showed an outstandi...

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Detalles Bibliográficos
Autores principales: Díaz Rodríguez, Rosario, Heredia, Gabriela, Siles, José A., Jurado, Miguel, Saparrat, Mario Carlos Nazareno, García Romera, Inmaculada, Sampedro, Inmaculada
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2019
Materias:
Biología
Laccase activity enhancement
Fungal interaction
Co-culture
White-rot fungus
Soil fungus
48 isoenzymes
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/125065
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-125065
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Laccase activity enhancement
Fungal interaction
Co-culture
White-rot fungus
Soil fungus
48 isoenzymes
spellingShingle Biología
Laccase activity enhancement
Fungal interaction
Co-culture
White-rot fungus
Soil fungus
48 isoenzymes
Díaz Rodríguez, Rosario
Heredia, Gabriela
Siles, José A.
Jurado, Miguel
Saparrat, Mario Carlos Nazareno
García Romera, Inmaculada
Sampedro, Inmaculada
Enhancing laccase production by white-rot fungus Funalia floccosa LPSC 232 in co-culture with Penicillium commune GHAIE86
topic_facet Biología
Laccase activity enhancement
Fungal interaction
Co-culture
White-rot fungus
Soil fungus
48 isoenzymes
description To obtain enzymatic preparations with higher laccase activity levels from Funalia floccosa LPSC 232, available for use in several applications, co-cultures with six filamentous microfungi were tested. A laccase non-producing soil fungus, identified as Penicillium commune GHAIE86, showed an outstanding ability to increase laccase activity (3-fold as compared to that for monoculture) when inoculated in 6-day-old F. floccosa cultures. Maximum laccase production with the F. floccosa and P. commune co-culture reached 60 U/mL, or twice that induced by chemical treatments alone. Our study demonstrated that co-culture with soil fungi might be a promising method for improving laccase production in F. floccosa. Although the enhancement of laccase activity was a function of P. commune inoculation time, two laccase isoenzymes produced by F. floccosa remained unchanged when strains were co-cultured. These data are compatible with the potential of F. floccosa in agricultural applications in soil, whose enzyme machinery could be activated by soil fungi such as P. commune.
format Articulo
Preprint
author Díaz Rodríguez, Rosario
Heredia, Gabriela
Siles, José A.
Jurado, Miguel
Saparrat, Mario Carlos Nazareno
García Romera, Inmaculada
Sampedro, Inmaculada
author_facet Díaz Rodríguez, Rosario
Heredia, Gabriela
Siles, José A.
Jurado, Miguel
Saparrat, Mario Carlos Nazareno
García Romera, Inmaculada
Sampedro, Inmaculada
author_sort Díaz Rodríguez, Rosario
title Enhancing laccase production by white-rot fungus Funalia floccosa LPSC 232 in co-culture with Penicillium commune GHAIE86
title_short Enhancing laccase production by white-rot fungus Funalia floccosa LPSC 232 in co-culture with Penicillium commune GHAIE86
title_full Enhancing laccase production by white-rot fungus Funalia floccosa LPSC 232 in co-culture with Penicillium commune GHAIE86
title_fullStr Enhancing laccase production by white-rot fungus Funalia floccosa LPSC 232 in co-culture with Penicillium commune GHAIE86
title_full_unstemmed Enhancing laccase production by white-rot fungus Funalia floccosa LPSC 232 in co-culture with Penicillium commune GHAIE86
title_sort enhancing laccase production by white-rot fungus funalia floccosa lpsc 232 in co-culture with penicillium commune ghaie86
publishDate 2019
url http://sedici.unlp.edu.ar/handle/10915/125065
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