Protection efficacy of Argentinian isolates of Mycobacterium avium subsp. paratuberculosis with different genotypes and virulence in a murine model

Paratuberculosis is a chronic disease caused by Mycobacterium avium subsp. paratuberculosis ( Map ). The disease causes economic losses and, therefore, it is imperative to follow proper control strategies, which should include an effective vaccine. Several strategies have assessed the virulence and...

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Detalles Bibliográficos
Autores principales: Colombatti Olivieri, María Alejandra, Moyano, Roberto Damian, Travería, Gabriel Eduardo, Alvarado Pinedo, María Fiorella, Mon, María Laura, Gravisaco, María José, Delgado, Fernando, Santangelo, María Paz, Romano, Maria Isabel
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2018
Materias:
Ciencias Veterinarias
Mycobacterium avium subsp. paratuberculosis
Virulence
Vaccine
Murine model
Immune response
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/124994
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-124994
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Mycobacterium avium subsp. paratuberculosis
Virulence
Vaccine
Murine model
Immune response
spellingShingle Ciencias Veterinarias
Mycobacterium avium subsp. paratuberculosis
Virulence
Vaccine
Murine model
Immune response
Colombatti Olivieri, María Alejandra
Moyano, Roberto Damian
Travería, Gabriel Eduardo
Alvarado Pinedo, María Fiorella
Mon, María Laura
Gravisaco, María José
Delgado, Fernando
Santangelo, María Paz
Romano, Maria Isabel
Protection efficacy of Argentinian isolates of Mycobacterium avium subsp. paratuberculosis with different genotypes and virulence in a murine model
topic_facet Ciencias Veterinarias
Mycobacterium avium subsp. paratuberculosis
Virulence
Vaccine
Murine model
Immune response
description Paratuberculosis is a chronic disease caused by Mycobacterium avium subsp. paratuberculosis ( Map ). The disease causes economic losses and, therefore, it is imperative to follow proper control strategies, which should include an effective vaccine. Several strategies have assessed the virulence and immune response of Map strains that could be used as a vaccine. This study evaluates the degree of virulence, immune response, and protection of Argentinian strains of Map with different genotype in a murine model. Four local isolates (Cattle type) with different genotypes (analyzed by MIRU-VNTR and SSRs) were selected and evaluated in a virulence assay in BALB/c mice. This assay allowed us to differentiate virulent and low-virulence Map strains. The less virulent strains (1543/481 and A162) failed to induce a significant production of the proinflammatory cytokine IFNg, whereas the virulent strain 6611 established infection along with a proinflammatory immune response. On the other hand, the virulent strain 1347/498 was efficient in establishing a persistent infection, but failed to promote an important Th1 response compared with 6611 at the evaluated time. We selected the low-virulence strain 1543/498 as a live vaccine and the virulent strain 6611 as a live and inactivated vaccine in a protection assay in mice. Strain 1543/481 failed to protect the animals from challenge, whereas strain 6611, in its live and inactivated form, significantly reduced the CFUs count in the infected mice, although they had different immunological response profiles. The inactivated virulent strain 6611 is a potential vaccine candidate against paratuberculosis to be tested in cattle.
format Articulo
Preprint
author Colombatti Olivieri, María Alejandra
Moyano, Roberto Damian
Travería, Gabriel Eduardo
Alvarado Pinedo, María Fiorella
Mon, María Laura
Gravisaco, María José
Delgado, Fernando
Santangelo, María Paz
Romano, Maria Isabel
author_facet Colombatti Olivieri, María Alejandra
Moyano, Roberto Damian
Travería, Gabriel Eduardo
Alvarado Pinedo, María Fiorella
Mon, María Laura
Gravisaco, María José
Delgado, Fernando
Santangelo, María Paz
Romano, Maria Isabel
author_sort Colombatti Olivieri, María Alejandra
title Protection efficacy of Argentinian isolates of Mycobacterium avium subsp. paratuberculosis with different genotypes and virulence in a murine model
title_short Protection efficacy of Argentinian isolates of Mycobacterium avium subsp. paratuberculosis with different genotypes and virulence in a murine model
title_full Protection efficacy of Argentinian isolates of Mycobacterium avium subsp. paratuberculosis with different genotypes and virulence in a murine model
title_fullStr Protection efficacy of Argentinian isolates of Mycobacterium avium subsp. paratuberculosis with different genotypes and virulence in a murine model
title_full_unstemmed Protection efficacy of Argentinian isolates of Mycobacterium avium subsp. paratuberculosis with different genotypes and virulence in a murine model
title_sort protection efficacy of argentinian isolates of mycobacterium avium subsp. paratuberculosis with different genotypes and virulence in a murine model
publishDate 2018
url http://sedici.unlp.edu.ar/handle/10915/124994
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