Photochemical formation of a fluorescent thymidine-pterin adduct in DNA

The photochemistry of the DNA biomacromolecule is an issue of paramount importance as it is part of the etiology of skin cancer development, being ultraviolet sunlight radiation the most relevant environmental carcinogen. Herein, we demonstrate the potential of pterin, an endogenous compound, to for...

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Detalles Bibliográficos
Autores principales: Estébanez Ruiz, Sandra, Lorente, Carolina, Gaspar Tosato, Maira, Miranda, Miguel A., Marin, M. Luisa, Lhiaubet Vallet, Virginie, Thomas, Andrés Héctor
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2019
Materias:
Química
Pterins
Thymine
Photosensitization
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/124992
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-124992
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Pterins
Thymine
Photosensitization
spellingShingle Química
Pterins
Thymine
Photosensitization
Estébanez Ruiz, Sandra
Lorente, Carolina
Gaspar Tosato, Maira
Miranda, Miguel A.
Marin, M. Luisa
Lhiaubet Vallet, Virginie
Thomas, Andrés Héctor
Photochemical formation of a fluorescent thymidine-pterin adduct in DNA
topic_facet Química
Pterins
Thymine
Photosensitization
description The photochemistry of the DNA biomacromolecule is an issue of paramount importance as it is part of the etiology of skin cancer development, being ultraviolet sunlight radiation the most relevant environmental carcinogen. Herein, we demonstrate the potential of pterin, an endogenous compound, to form covalent adduct under UVA irradiation with a short thymine oligomer as well as with the whole DNA polymer. Our approach is based on the spectroscopic features of pterin, which allow, by monitoring specific absorption or emission wavelengths, the following-up of the covalent binding. The results are confirmed by HPLC coupled with mass spectrometry, revealing the attachment of one or two pterin units to the homothymine 5-mer oligonucleotide. Altogether the findings point toward the role of pterin as endogenous sensitizer and genotoxic compound.
format Articulo
Preprint
author Estébanez Ruiz, Sandra
Lorente, Carolina
Gaspar Tosato, Maira
Miranda, Miguel A.
Marin, M. Luisa
Lhiaubet Vallet, Virginie
Thomas, Andrés Héctor
author_facet Estébanez Ruiz, Sandra
Lorente, Carolina
Gaspar Tosato, Maira
Miranda, Miguel A.
Marin, M. Luisa
Lhiaubet Vallet, Virginie
Thomas, Andrés Héctor
author_sort Estébanez Ruiz, Sandra
title Photochemical formation of a fluorescent thymidine-pterin adduct in DNA
title_short Photochemical formation of a fluorescent thymidine-pterin adduct in DNA
title_full Photochemical formation of a fluorescent thymidine-pterin adduct in DNA
title_fullStr Photochemical formation of a fluorescent thymidine-pterin adduct in DNA
title_full_unstemmed Photochemical formation of a fluorescent thymidine-pterin adduct in DNA
title_sort photochemical formation of a fluorescent thymidine-pterin adduct in dna
publishDate 2019
url http://sedici.unlp.edu.ar/handle/10915/124992
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AT gaspartosatomaira photochemicalformationofafluorescentthymidinepterinadductindna
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