Molecular Characterization of Differences between the Tomato Immune Receptors Flagellin Sensing 3 and Flagellin Sensing 2

Plants mount defense responses by recognizing indications of pathogen invasion, including microbe-associated molecular patterns (MAMPs). Flagellin from the bacterial pathogen Pseudomonas syringae pv. tomato (Pst) contains two MAMPs, flg22 and flgII-28, that are recognized by tomato receptors Flagell...

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Detalles Bibliográficos
Autores principales:	Roberts, Robyn, Liu, Alexander E., Wan, Lingwei, Geiger, Annie M., Hind, Sarah R., Rosli, Hernán Guillermo, Martin, Gregory B.
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2020
Materias:	Botánica Plant immunity Flagellin PRR-triggered immunity Bacterial speck disease Tomato
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/124986
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-124986
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Botánica Plant immunity Flagellin PRR-triggered immunity Bacterial speck disease Tomato
spellingShingle	Botánica Plant immunity Flagellin PRR-triggered immunity Bacterial speck disease Tomato Roberts, Robyn Liu, Alexander E. Wan, Lingwei Geiger, Annie M. Hind, Sarah R. Rosli, Hernán Guillermo Martin, Gregory B. Molecular Characterization of Differences between the Tomato Immune Receptors Flagellin Sensing 3 and Flagellin Sensing 2
topic_facet	Botánica Plant immunity Flagellin PRR-triggered immunity Bacterial speck disease Tomato
description	Plants mount defense responses by recognizing indications of pathogen invasion, including microbe-associated molecular patterns (MAMPs). Flagellin from the bacterial pathogen Pseudomonas syringae pv. tomato (Pst) contains two MAMPs, flg22 and flgII-28, that are recognized by tomato receptors Flagellin sensing 2 (Fls2) and Flagellin sensing 3 (Fls3), respectively. It is unknown to what degree each receptor contributes to immunity and if they promote immune responses using the same molecular mechanisms. Characterization of CRISPR/Cas9-generated Fls2 and Fls3 tomato mutants revealed that the two receptors contribute equally to disease resistance both on the leaf surface and in the apoplast. However, striking differences were observed in certain host responses mediated by the two receptors. Compared to Fls2, Fls3 mediated a more sustained production of reactive oxygen species (ROS) and an increase in transcript abundance of 44 tomato genes, with two genes serving as reporters for Fls3. Fls3 had greater in vitro kinase activity and interacted differently with the Pst effector AvrPtoB as compared to Fls2. Using chimeric Fls2/Fls3 proteins, we found that no receptor domain was solely responsible for the Fls3 sustained ROS, suggesting involvement of multiple structural features. This work reveals differences in the immunity outputs between Fls2 and Fls3, suggesting they use distinct molecular mechanisms to activate pattern-triggered immunity in response to flagellin-derived MAMPs.
format	Articulo Articulo
author	Roberts, Robyn Liu, Alexander E. Wan, Lingwei Geiger, Annie M. Hind, Sarah R. Rosli, Hernán Guillermo Martin, Gregory B.
author_facet	Roberts, Robyn Liu, Alexander E. Wan, Lingwei Geiger, Annie M. Hind, Sarah R. Rosli, Hernán Guillermo Martin, Gregory B.
author_sort	Roberts, Robyn
title	Molecular Characterization of Differences between the Tomato Immune Receptors Flagellin Sensing 3 and Flagellin Sensing 2
title_short	Molecular Characterization of Differences between the Tomato Immune Receptors Flagellin Sensing 3 and Flagellin Sensing 2
title_full	Molecular Characterization of Differences between the Tomato Immune Receptors Flagellin Sensing 3 and Flagellin Sensing 2
title_fullStr	Molecular Characterization of Differences between the Tomato Immune Receptors Flagellin Sensing 3 and Flagellin Sensing 2
title_full_unstemmed	Molecular Characterization of Differences between the Tomato Immune Receptors Flagellin Sensing 3 and Flagellin Sensing 2
title_sort	molecular characterization of differences between the tomato immune receptors flagellin sensing 3 and flagellin sensing 2
publishDate	2020
url	http://sedici.unlp.edu.ar/handle/10915/124986
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bdutipo_str	Repositorios
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Molecular Characterization of Differences between the Tomato Immune Receptors Flagellin Sensing 3 and Flagellin Sensing 2

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