Probiotic Lactobacilli Isolated from Kefir Promote Down-Regulation of Inflammatory Lamina Propria T Cells from Patients with Active IBD
Ulcerative colitis and Crohn’s disease, the two main forms of inflammatory bowel disease (IBD), are immunologically mediated disorders. Several therapies are focused on activated T cells as key targets. Although Lactobacillus kefiri has shown anti-inflammatory effects in animal models, few studies w...
| Autores principales: | , , , , , , , , , , , , , , , , |
|---|---|
| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2021
|
| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/124859 |
| Aporte de: |
| id |
I19-R120-10915-124859 |
|---|---|
| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Biología Bioquímica Ulcerative colitis Crohn’s disease Probiotics Immunomodulation Mucosal samples Lactobacillus kefiri CIDCA 8348 |
| spellingShingle |
Biología Bioquímica Ulcerative colitis Crohn’s disease Probiotics Immunomodulation Mucosal samples Lactobacillus kefiri CIDCA 8348 Curciarello, Renata Canziani, Karina Eva Salto, Ileana Paula Barbiera Romero, Emanuel Rocca, Andrés Doldan, Ivan Peton, Emmanuel Brayer, Santiago Sambuelli, Alicia M. Goncalves, Silvina A. Tirado, Pablo Correa, Gustavo Yantorno, Martin Garbi, Laura Docena, Guillermo Horacio Serradell, María de los Ángeles Muglia, Cecilia Isabel Probiotic Lactobacilli Isolated from Kefir Promote Down-Regulation of Inflammatory Lamina Propria T Cells from Patients with Active IBD |
| topic_facet |
Biología Bioquímica Ulcerative colitis Crohn’s disease Probiotics Immunomodulation Mucosal samples Lactobacillus kefiri CIDCA 8348 |
| description |
Ulcerative colitis and Crohn’s disease, the two main forms of inflammatory bowel disease (IBD), are immunologically mediated disorders. Several therapies are focused on activated T cells as key targets. Although Lactobacillus kefiri has shown anti-inflammatory effects in animal models, few studies were done using human mucosal T cells. The aim of this work was to investigate the immunomodulatory effects of this bacterium on intestinal T cells from patients with active IBD. Mucosal biopsies and surgical samples from IBD adult patients (n = 19) or healthy donors (HC; n = 5) were used. Lamina propria mononuclear cells were isolated by enzymatic tissue digestion, and entero-adhesive Escherichia coli-specific lamina propria T cells (LPTC) were expanded. The immunomodulatory properties of L. kefiri CIDCA 8348 strain were evaluated on biopsies and on anti-CD3/CD28-activated LPTC. Secreted cytokines were quantified by ELISA, and cell proliferation and viability were assessed by flow cytometry. We found that L. kefiri reduced spontaneous release of IL-6 and IL-8 from inflamed biopsies ex vivo. Activated LPTC from IBD patients showed low proliferative rates and reduced secretion of TNF-α, IL-6, IFN-γ and IL-13 in the presence of L. kefiri. In addition, L. kefiri induced an increased frequency of CD4+FOXP3+ LPTC along with high levels of IL-10. This is the first report showing an immunomodulatory effect of L. kefiri CIDCA 8348 on human intestinal cells from IBD patients. Understanding the mechanisms of interaction between probiotics and immune mucosal cells may open new avenues for treatment and prevention of IBD. |
| format |
Articulo Articulo |
| author |
Curciarello, Renata Canziani, Karina Eva Salto, Ileana Paula Barbiera Romero, Emanuel Rocca, Andrés Doldan, Ivan Peton, Emmanuel Brayer, Santiago Sambuelli, Alicia M. Goncalves, Silvina A. Tirado, Pablo Correa, Gustavo Yantorno, Martin Garbi, Laura Docena, Guillermo Horacio Serradell, María de los Ángeles Muglia, Cecilia Isabel |
| author_facet |
Curciarello, Renata Canziani, Karina Eva Salto, Ileana Paula Barbiera Romero, Emanuel Rocca, Andrés Doldan, Ivan Peton, Emmanuel Brayer, Santiago Sambuelli, Alicia M. Goncalves, Silvina A. Tirado, Pablo Correa, Gustavo Yantorno, Martin Garbi, Laura Docena, Guillermo Horacio Serradell, María de los Ángeles Muglia, Cecilia Isabel |
| author_sort |
Curciarello, Renata |
| title |
Probiotic Lactobacilli Isolated from Kefir Promote Down-Regulation of Inflammatory Lamina Propria T Cells from Patients with Active IBD |
| title_short |
Probiotic Lactobacilli Isolated from Kefir Promote Down-Regulation of Inflammatory Lamina Propria T Cells from Patients with Active IBD |
| title_full |
Probiotic Lactobacilli Isolated from Kefir Promote Down-Regulation of Inflammatory Lamina Propria T Cells from Patients with Active IBD |
| title_fullStr |
Probiotic Lactobacilli Isolated from Kefir Promote Down-Regulation of Inflammatory Lamina Propria T Cells from Patients with Active IBD |
| title_full_unstemmed |
Probiotic Lactobacilli Isolated from Kefir Promote Down-Regulation of Inflammatory Lamina Propria T Cells from Patients with Active IBD |
| title_sort |
probiotic lactobacilli isolated from kefir promote down-regulation of inflammatory lamina propria t cells from patients with active ibd |
| publishDate |
2021 |
| url |
http://sedici.unlp.edu.ar/handle/10915/124859 |
| work_keys_str_mv |
AT curciarellorenata probioticlactobacilliisolatedfromkefirpromotedownregulationofinflammatorylaminapropriatcellsfrompatientswithactiveibd AT canzianikarinaeva probioticlactobacilliisolatedfromkefirpromotedownregulationofinflammatorylaminapropriatcellsfrompatientswithactiveibd AT saltoileanapaula probioticlactobacilliisolatedfromkefirpromotedownregulationofinflammatorylaminapropriatcellsfrompatientswithactiveibd AT barbieraromeroemanuel probioticlactobacilliisolatedfromkefirpromotedownregulationofinflammatorylaminapropriatcellsfrompatientswithactiveibd AT roccaandres probioticlactobacilliisolatedfromkefirpromotedownregulationofinflammatorylaminapropriatcellsfrompatientswithactiveibd AT doldanivan probioticlactobacilliisolatedfromkefirpromotedownregulationofinflammatorylaminapropriatcellsfrompatientswithactiveibd AT petonemmanuel probioticlactobacilliisolatedfromkefirpromotedownregulationofinflammatorylaminapropriatcellsfrompatientswithactiveibd AT brayersantiago probioticlactobacilliisolatedfromkefirpromotedownregulationofinflammatorylaminapropriatcellsfrompatientswithactiveibd AT sambuellialiciam probioticlactobacilliisolatedfromkefirpromotedownregulationofinflammatorylaminapropriatcellsfrompatientswithactiveibd AT goncalvessilvinaa probioticlactobacilliisolatedfromkefirpromotedownregulationofinflammatorylaminapropriatcellsfrompatientswithactiveibd AT tiradopablo probioticlactobacilliisolatedfromkefirpromotedownregulationofinflammatorylaminapropriatcellsfrompatientswithactiveibd AT correagustavo probioticlactobacilliisolatedfromkefirpromotedownregulationofinflammatorylaminapropriatcellsfrompatientswithactiveibd AT yantornomartin probioticlactobacilliisolatedfromkefirpromotedownregulationofinflammatorylaminapropriatcellsfrompatientswithactiveibd AT garbilaura probioticlactobacilliisolatedfromkefirpromotedownregulationofinflammatorylaminapropriatcellsfrompatientswithactiveibd AT docenaguillermohoracio probioticlactobacilliisolatedfromkefirpromotedownregulationofinflammatorylaminapropriatcellsfrompatientswithactiveibd AT serradellmariadelosangeles probioticlactobacilliisolatedfromkefirpromotedownregulationofinflammatorylaminapropriatcellsfrompatientswithactiveibd AT mugliaceciliaisabel probioticlactobacilliisolatedfromkefirpromotedownregulationofinflammatorylaminapropriatcellsfrompatientswithactiveibd |
| bdutipo_str |
Repositorios |
| _version_ |
1764820451225239552 |