DNA Oxidation Photoinduced by Norharmane Rhenium(I) Polypyridyl Complexes: Effect of the Bidentate N,N'-Ligands on the Damage Profile

Re(I)--polypyridyl complexes have interesting and distinctive photochemical and photosensitizing properties. This work describes the capability to induce (or photoinduce) DNA damage of three Re(I)-complexes with a naturally occurring alkaloid called norharmane (nHo) as ligand: [Re(CO)₃ (nHo)(L)]CF₃...

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Autores principales: Maisuls, Iván, Cabrerizo, Franco Martín, David Gara, Pedro Maximiliano, Epe, Bernd, Ruiz, Gustavo Teodosio
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2018
Materias:
Química
Adduct
Electron transfer
Dna damage
Denticity
Chemistry
Phenanthroline
Phenazine
Dna
Stereochemistry
Ligand
Dna cleavage
Alkaloids
Photosensitization
Rhenium
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/124856
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-124856
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Adduct
Electron transfer
Dna damage
Denticity
Chemistry
Phenanthroline
Phenazine
Dna
Stereochemistry
Ligand
Dna cleavage
Alkaloids
Photosensitization
Rhenium
spellingShingle Química
Adduct
Electron transfer
Dna damage
Denticity
Chemistry
Phenanthroline
Phenazine
Dna
Stereochemistry
Ligand
Dna cleavage
Alkaloids
Photosensitization
Rhenium
Maisuls, Iván
Cabrerizo, Franco Martín
David Gara, Pedro Maximiliano
Epe, Bernd
Ruiz, Gustavo Teodosio
DNA Oxidation Photoinduced by Norharmane Rhenium(I) Polypyridyl Complexes: Effect of the Bidentate N,N'-Ligands on the Damage Profile
topic_facet Química
Adduct
Electron transfer
Dna damage
Denticity
Chemistry
Phenanthroline
Phenazine
Dna
Stereochemistry
Ligand
Dna cleavage
Alkaloids
Photosensitization
Rhenium
description Re(I)--polypyridyl complexes have interesting and distinctive photochemical and photosensitizing properties. This work describes the capability to induce (or photoinduce) DNA damage of three Re(I)-complexes with a naturally occurring alkaloid called norharmane (nHo) as ligand: [Re(CO)₃ (nHo)(L)]CF₃ SO₃ where L=2,2'-bipyridine (ReBpy), phenanthroline (RePhen) or dipyrido[3,2-a:2',3'-c]phenazine (ReDppz). The interaction of the complexes with DNA was investigated by steady-state and time-resolved spectroscopy. Data show that the mode and strength of interaction depend on the chemical structure of the bidentate ligand. The complexes show a major static contribution to the overall interaction, giving rise to the formation of noncovalent adducts with DNA, and the particular trend observed was RePhen>ReDppz>ReBpy. Photo-oxidation at the purine bases represents the major DNA damaging mechanism. RePhen also induces single-strand breaks in a yield similar to that of base damage, suggesting an additional photosensitizing pathway. We also performed the Ames test to evaluate the cytotoxic and mutagenic properties of both non-irradiated and photoexcited complexes. RePhen, but not the other complexes, turned out to be both toxic and phototoxic for the bacteria.
format Articulo
Preprint
author Maisuls, Iván
Cabrerizo, Franco Martín
David Gara, Pedro Maximiliano
Epe, Bernd
Ruiz, Gustavo Teodosio
author_facet Maisuls, Iván
Cabrerizo, Franco Martín
David Gara, Pedro Maximiliano
Epe, Bernd
Ruiz, Gustavo Teodosio
author_sort Maisuls, Iván
title DNA Oxidation Photoinduced by Norharmane Rhenium(I) Polypyridyl Complexes: Effect of the Bidentate N,N'-Ligands on the Damage Profile
title_short DNA Oxidation Photoinduced by Norharmane Rhenium(I) Polypyridyl Complexes: Effect of the Bidentate N,N'-Ligands on the Damage Profile
title_full DNA Oxidation Photoinduced by Norharmane Rhenium(I) Polypyridyl Complexes: Effect of the Bidentate N,N'-Ligands on the Damage Profile
title_fullStr DNA Oxidation Photoinduced by Norharmane Rhenium(I) Polypyridyl Complexes: Effect of the Bidentate N,N'-Ligands on the Damage Profile
title_full_unstemmed DNA Oxidation Photoinduced by Norharmane Rhenium(I) Polypyridyl Complexes: Effect of the Bidentate N,N'-Ligands on the Damage Profile
title_sort dna oxidation photoinduced by norharmane rhenium(i) polypyridyl complexes: effect of the bidentate n,n'-ligands on the damage profile
publishDate 2018
url http://sedici.unlp.edu.ar/handle/10915/124856
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