Molecular detection of honeybee viruses in Ecuador

Aim of study: The honeybee, Apis mellifera, is one of the most important pollinators in the world. Apicultural activity and pollination services have been affected by the decline in the honeybee population, which may be due to the interaction of multiple risk factors, such as changes in agricultural...

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Detalles Bibliográficos
Autores principales: Bravi, María Emilia, Avalos, Jorge, Rosero, Hugo, Maldonado, Gerald, Reynaldi, Francisco José, Genchi García, María Laura
Formato: Articulo Comunicacion
Lenguaje:Inglés
Publicado: 2020
Materias:
Biología
Ciencias Agrarias
Acute Bee Paralysis Virus
(Deformed Wing Virus
Sacbrood Virus
(Black Queen Cell Virus
Multiplex-PCR,
Apis mellifera
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/124599
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-124599
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Ciencias Agrarias
Acute Bee Paralysis Virus
(Deformed Wing Virus
Sacbrood Virus
(Black Queen Cell Virus
Multiplex-PCR,
Apis mellifera
spellingShingle Biología
Ciencias Agrarias
Acute Bee Paralysis Virus
(Deformed Wing Virus
Sacbrood Virus
(Black Queen Cell Virus
Multiplex-PCR,
Apis mellifera
Bravi, María Emilia
Avalos, Jorge
Rosero, Hugo
Maldonado, Gerald
Reynaldi, Francisco José
Genchi García, María Laura
Molecular detection of honeybee viruses in Ecuador
topic_facet Biología
Ciencias Agrarias
Acute Bee Paralysis Virus
(Deformed Wing Virus
Sacbrood Virus
(Black Queen Cell Virus
Multiplex-PCR,
Apis mellifera
description Aim of study: The honeybee, Apis mellifera, is one of the most important pollinators in the world. Apicultural activity and pollination services have been affected by the decline in the honeybee population, which may be due to the interaction of multiple risk factors, such as changes in agricultural production, use of pesticides and presence of pathogens. Viruses, in particular, are suspected to be drivers of colony mortality. In this scenario, the aim of this study was to determine the presence of honeybee viruses (IAPV, DWV, SBV, ABPV, BQCV, CBPV) in A. mellifera populations using a RT-mPCR assay.Area of study: Apiaries were situated in Pichincha, Ecuador.Material and methods: Samples were collected from seventeen apiaries that exhibited mortality but without specific clinical signs. Each sample comprised 15 individuals. After RNA extraction, a multiplex PCR analysis was performed for presence of six viruses (IAPV, DWV, SBV, ABPV, BQCV, CBPV).Main results: Four of the viruses (ABPV, DWV, BQCV and SBV) were found in co-infections in these colonies, with ABPV and SBV also being found in simple infections.Research highlights: To our knowledge, this is the first molecular detection of BQCV and SBV in Ecuador. These findings suggest that some of the above viruses could be involved in weakening these colonies.
format Articulo
Comunicacion
author Bravi, María Emilia
Avalos, Jorge
Rosero, Hugo
Maldonado, Gerald
Reynaldi, Francisco José
Genchi García, María Laura
author_facet Bravi, María Emilia
Avalos, Jorge
Rosero, Hugo
Maldonado, Gerald
Reynaldi, Francisco José
Genchi García, María Laura
author_sort Bravi, María Emilia
title Molecular detection of honeybee viruses in Ecuador
title_short Molecular detection of honeybee viruses in Ecuador
title_full Molecular detection of honeybee viruses in Ecuador
title_fullStr Molecular detection of honeybee viruses in Ecuador
title_full_unstemmed Molecular detection of honeybee viruses in Ecuador
title_sort molecular detection of honeybee viruses in ecuador
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/124599
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bdutipo_str Repositorios
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