Nanostructured fumarate copolymer-chitosan crosslinked scaffold: An in vitro osteochondrogenesis regeneration study

In the tissue engineering field, the design of the scaffold inspired on the natural occurring tissue is of vital importance. Ideally, the scaffold surface must promote cell growth and differentiation, while promote angiogenesis in the in vivo implant of the scaffold. On the other hand, the material...

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Detalles Bibliográficos
Autores principales:	Lastra, María Laura, Molinuevo, María Silvina, Blaszczyk Lezak, Iwona, Mijangos, Carmen, Cortizo, María Susana
Formato:	Articulo Preprint
Lenguaje:	Inglés
Publicado:	2017
Materias:	Ciencias Exactas Cytotoxicity Biomedical engineering Regeneration (biology) Materials science Biocompatibility Chitosan Bone regeneration Tissue engineering Scaffold Nanofiber Cartilage regeneration Nanostructured biomaterials Polyfumarate
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/124415
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-124415
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Exactas Cytotoxicity Biomedical engineering Regeneration (biology) Materials science Biocompatibility Chitosan Bone regeneration Tissue engineering Scaffold Nanofiber Cartilage regeneration Nanostructured biomaterials Polyfumarate
spellingShingle	Ciencias Exactas Cytotoxicity Biomedical engineering Regeneration (biology) Materials science Biocompatibility Chitosan Bone regeneration Tissue engineering Scaffold Nanofiber Cartilage regeneration Nanostructured biomaterials Polyfumarate Lastra, María Laura Molinuevo, María Silvina Blaszczyk Lezak, Iwona Mijangos, Carmen Cortizo, María Susana Nanostructured fumarate copolymer-chitosan crosslinked scaffold: An in vitro osteochondrogenesis regeneration study
topic_facet	Ciencias Exactas Cytotoxicity Biomedical engineering Regeneration (biology) Materials science Biocompatibility Chitosan Bone regeneration Tissue engineering Scaffold Nanofiber Cartilage regeneration Nanostructured biomaterials Polyfumarate
description	In the tissue engineering field, the design of the scaffold inspired on the natural occurring tissue is of vital importance. Ideally, the scaffold surface must promote cell growth and differentiation, while promote angiogenesis in the in vivo implant of the scaffold. On the other hand, the material selection must be biocompatible and the degradation times should meet tissue reparation times. In the present work, we developed a nanofibrous scaffold based on chitosan crosslinked with diisopropylfumarate-vinyl acetate copolymer using anodized aluminum oxide (AAO) templates. We have previously demonstrated its biocompatibility properties with low cytotoxicity and proper degradation times. Now, we extended our studies to demonstrate that it can be successfully nanostructured using the AAO templates methodology, obtaining a nanorod-like scaffold with a diameter comparable to those of collagen fibers of the bone matrix (170 and 300 nm). The nanorods obtained presented a very homogeneous pattern in diameter and length, and supports cell attachment and growth. We also found that both osteoblastic and chondroblastic matrix production were promoted on bone marrow progenitor cells and primary condrocytes growing on the scaffolds, respectively. In addition, the nanostructured scaffold presented no cytotoxicity as it was evaluated using a model of macrophages on culture.
format	Articulo Preprint
author	Lastra, María Laura Molinuevo, María Silvina Blaszczyk Lezak, Iwona Mijangos, Carmen Cortizo, María Susana
author_facet	Lastra, María Laura Molinuevo, María Silvina Blaszczyk Lezak, Iwona Mijangos, Carmen Cortizo, María Susana
author_sort	Lastra, María Laura
title	Nanostructured fumarate copolymer-chitosan crosslinked scaffold: An in vitro osteochondrogenesis regeneration study
title_short	Nanostructured fumarate copolymer-chitosan crosslinked scaffold: An in vitro osteochondrogenesis regeneration study
title_full	Nanostructured fumarate copolymer-chitosan crosslinked scaffold: An in vitro osteochondrogenesis regeneration study
title_fullStr	Nanostructured fumarate copolymer-chitosan crosslinked scaffold: An in vitro osteochondrogenesis regeneration study
title_full_unstemmed	Nanostructured fumarate copolymer-chitosan crosslinked scaffold: An in vitro osteochondrogenesis regeneration study
title_sort	nanostructured fumarate copolymer-chitosan crosslinked scaffold: an in vitro osteochondrogenesis regeneration study
publishDate	2017
url	http://sedici.unlp.edu.ar/handle/10915/124415
work_keys_str_mv	AT lastramarialaura nanostructuredfumaratecopolymerchitosancrosslinkedscaffoldaninvitroosteochondrogenesisregenerationstudy AT molinuevomariasilvina nanostructuredfumaratecopolymerchitosancrosslinkedscaffoldaninvitroosteochondrogenesisregenerationstudy AT blaszczyklezakiwona nanostructuredfumaratecopolymerchitosancrosslinkedscaffoldaninvitroosteochondrogenesisregenerationstudy AT mijangoscarmen nanostructuredfumaratecopolymerchitosancrosslinkedscaffoldaninvitroosteochondrogenesisregenerationstudy AT cortizomariasusana nanostructuredfumaratecopolymerchitosancrosslinkedscaffoldaninvitroosteochondrogenesisregenerationstudy
bdutipo_str	Repositorios
_version_	1764820450318221312

Nanostructured fumarate copolymer-chitosan crosslinked scaffold: An in vitro osteochondrogenesis regeneration study

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