Non-digestible proteins and protease inhibitors: implications for defense of the colored eggs of the freshwater apple snail Pomacea canaliculata

Apple snails (Pomacea Perry, 1810) are successful invaders that cause ecological perturbations, economic losses, and medical issues. A peculiar trait of this snail is a high biological potential, related to the absence of predators of their eggs. Eggs show protease inhibitor (PI) activity, originall...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ituarte, Santiago, Brola, Tabata Romina, Dreon, Marcos Sebastián, Sun, Jin, Qiu, Jian-Wen, Heras, Horacio
Formato:	Articulo Preprint
Lenguaje:	Inglés
Publicado:	2019
Materias:	Zoología Protease inhibitor Invasive species Snail Pomacea Predation defenses Egg defenses
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/124301
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-124301
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Zoología Protease inhibitor Invasive species Snail Pomacea Predation defenses Egg defenses
spellingShingle	Zoología Protease inhibitor Invasive species Snail Pomacea Predation defenses Egg defenses Ituarte, Santiago Brola, Tabata Romina Dreon, Marcos Sebastián Sun, Jin Qiu, Jian-Wen Heras, Horacio Non-digestible proteins and protease inhibitors: implications for defense of the colored eggs of the freshwater apple snail Pomacea canaliculata
topic_facet	Zoología Protease inhibitor Invasive species Snail Pomacea Predation defenses Egg defenses
description	Apple snails (Pomacea Perry, 1810) are successful invaders that cause ecological perturbations, economic losses, and medical issues. A peculiar trait of this snail is a high biological potential, related to the absence of predators of their eggs. Eggs show protease inhibitor (PI) activity, originally ascribed to PcOvo perivitellin in the apple snail Pomacea canaliculata (Lamarck, 1822) but absent in PmPV1, the orthologoue of PcOvo, in eggs of the apple snail Pomacea maculata Perry, 1810. As egg fluid diminishes rat growth rate, an antidigestive effect, similar to plant defenses against herbivory, was hypothesized. However, PI activity has not been characterized in apple snail eggs. Here we identify and partially characterize P. canaliculata egg PI and improve our knowledge of the quaternary structure and evolution of PcOvo. Through N-terminal, transcriptomic or proteomic sequencing, and biochemical validation, we identified a Kunitz-type and a Kazal-type inhibitor that, though at low concentration in the egg, exhibit strong PI activity against trypsin, chymotrypsin, elastase, and subtilisin. Additionally, we report three new subunits for the non-digestible storage protein PcOvo. They are likely products of ancient gene duplication, as their sequences exhibit moderate similarity (30%). To our knowledge, this is the first report of Kazal-type inhibition among invertebrate eggs. Inhibiting varied proteases, PI seems an efficient adaptive trait that limits predator’s capacity to digest egg nutrients.
format	Articulo Preprint
author	Ituarte, Santiago Brola, Tabata Romina Dreon, Marcos Sebastián Sun, Jin Qiu, Jian-Wen Heras, Horacio
author_facet	Ituarte, Santiago Brola, Tabata Romina Dreon, Marcos Sebastián Sun, Jin Qiu, Jian-Wen Heras, Horacio
author_sort	Ituarte, Santiago
title	Non-digestible proteins and protease inhibitors: implications for defense of the colored eggs of the freshwater apple snail Pomacea canaliculata
title_short	Non-digestible proteins and protease inhibitors: implications for defense of the colored eggs of the freshwater apple snail Pomacea canaliculata
title_full	Non-digestible proteins and protease inhibitors: implications for defense of the colored eggs of the freshwater apple snail Pomacea canaliculata
title_fullStr	Non-digestible proteins and protease inhibitors: implications for defense of the colored eggs of the freshwater apple snail Pomacea canaliculata
title_full_unstemmed	Non-digestible proteins and protease inhibitors: implications for defense of the colored eggs of the freshwater apple snail Pomacea canaliculata
title_sort	non-digestible proteins and protease inhibitors: implications for defense of the colored eggs of the freshwater apple snail pomacea canaliculata
publishDate	2019
url	http://sedici.unlp.edu.ar/handle/10915/124301
work_keys_str_mv	AT ituartesantiago nondigestibleproteinsandproteaseinhibitorsimplicationsfordefenseofthecoloredeggsofthefreshwaterapplesnailpomaceacanaliculata AT brolatabataromina nondigestibleproteinsandproteaseinhibitorsimplicationsfordefenseofthecoloredeggsofthefreshwaterapplesnailpomaceacanaliculata AT dreonmarcossebastian nondigestibleproteinsandproteaseinhibitorsimplicationsfordefenseofthecoloredeggsofthefreshwaterapplesnailpomaceacanaliculata AT sunjin nondigestibleproteinsandproteaseinhibitorsimplicationsfordefenseofthecoloredeggsofthefreshwaterapplesnailpomaceacanaliculata AT qiujianwen nondigestibleproteinsandproteaseinhibitorsimplicationsfordefenseofthecoloredeggsofthefreshwaterapplesnailpomaceacanaliculata AT herashoracio nondigestibleproteinsandproteaseinhibitorsimplicationsfordefenseofthecoloredeggsofthefreshwaterapplesnailpomaceacanaliculata
bdutipo_str	Repositorios
_version_	1764820450067611651

Non-digestible proteins and protease inhibitors: implications for defense of the colored eggs of the freshwater apple snail Pomacea canaliculata

Ejemplares similares