A <i>gua</i>B mutant strain of <i>Rhizobium tropici</i> CIAT899 pleiotropically defective in thermal tolerance and symbiosis

<i>Rhizobium tropici</i> strain CIAT899 displays a high intrinsic thermal tolerance, and had been used in this work to study the molecular basis of bacterial responses to high temperature. We generated a collection of <i>R. tropici</i> CIAT899 mutants affected in thermal tole...

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Autores principales: Riccillo, Pablo Miguel, Collavino, Mónica Mariana, Grasso, Daniel Horacio, England, Reg, Bruijn, Frans J. de, Aguilar, Orlando Mario
Formato: Articulo
Lenguaje:Inglés
Publicado: 2000
Materias:
Ciencias Exactas
Biología
guanine biosynthesis
nodule development
prototrophy
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/124099
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-124099
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Biología
guanine biosynthesis
nodule development
prototrophy
spellingShingle Ciencias Exactas
Biología
guanine biosynthesis
nodule development
prototrophy
Riccillo, Pablo Miguel
Collavino, Mónica Mariana
Grasso, Daniel Horacio
England, Reg
Bruijn, Frans J. de
Aguilar, Orlando Mario
A <i>gua</i>B mutant strain of <i>Rhizobium tropici</i> CIAT899 pleiotropically defective in thermal tolerance and symbiosis
topic_facet Ciencias Exactas
Biología
guanine biosynthesis
nodule development
prototrophy
description <i>Rhizobium tropici</i> strain CIAT899 displays a high intrinsic thermal tolerance, and had been used in this work to study the molecular basis of bacterial responses to high temperature. We generated a collection of <i>R. tropici</i> CIAT899 mutants affected in thermal tolerance using Tn5-<i>lux</i>AB mutagenesis and described the characterization of a mutant strain, CIAT899-10T, that fails to grow under conditions of high temperature. Strain CIAT899-10T carries a single transposon insertion in a gene showing a high degree of similarity with the <i>gua</i>B gene of <i>Escherichia coli</i> and other organisms, encoding the enzyme inosine monophosphate dehydrogenase. The <i>gua</i>B strain CIAT899-10T does not require guanine for growth due to an alternative pathway via xanthine dehydrogenase and, phenotypically, in addition to the thermal sensitivity, the mutant is also defective in symbiosis with beans, forming nodules that lack rhizobial content. Guanine and its precursors restore wild-type tolerance to grow at high temperature. Our data show that, in <i>R. tropici</i>, the production of guanine via inosine monophosphate dehydrogenase is essential for growth at extreme temperatures and for effective nodulation.
format Articulo
Articulo
author Riccillo, Pablo Miguel
Collavino, Mónica Mariana
Grasso, Daniel Horacio
England, Reg
Bruijn, Frans J. de
Aguilar, Orlando Mario
author_facet Riccillo, Pablo Miguel
Collavino, Mónica Mariana
Grasso, Daniel Horacio
England, Reg
Bruijn, Frans J. de
Aguilar, Orlando Mario
author_sort Riccillo, Pablo Miguel
title A <i>gua</i>B mutant strain of <i>Rhizobium tropici</i> CIAT899 pleiotropically defective in thermal tolerance and symbiosis
title_short A <i>gua</i>B mutant strain of <i>Rhizobium tropici</i> CIAT899 pleiotropically defective in thermal tolerance and symbiosis
title_full A <i>gua</i>B mutant strain of <i>Rhizobium tropici</i> CIAT899 pleiotropically defective in thermal tolerance and symbiosis
title_fullStr A <i>gua</i>B mutant strain of <i>Rhizobium tropici</i> CIAT899 pleiotropically defective in thermal tolerance and symbiosis
title_full_unstemmed A <i>gua</i>B mutant strain of <i>Rhizobium tropici</i> CIAT899 pleiotropically defective in thermal tolerance and symbiosis
title_sort <i>gua</i>b mutant strain of <i>rhizobium tropici</i> ciat899 pleiotropically defective in thermal tolerance and symbiosis
publishDate 2000
url http://sedici.unlp.edu.ar/handle/10915/124099
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