<i>Toxoplasma gondii</i> genotyping from free-range chickens (<i>Gallus gallus domesticus</i>) in a rural area of Rio Grande do Sul, Brazil

Free-range chickens may ingest oocysts of T. gondii present in the environment and consequently harbor virulent strains of this parasite in different tissues, without any clinical signs. Isolation of T. gondii through bioassays on mice and cats from naturally infected chicken tissues has been descri...

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Autores principales: Camillo, Giovana, Machado, M. E. A., Cadore, Gustavo Cauduro, Bräunig, Patricia, Venturini, María Cecilia, Pardini, Lais Luján, Barros, Luiz Daniel de, Garcia, João Luis, Sangioni, L. A., Vogel, F. S. F.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2020
Materias:
Ciencias Veterinarias
Genotyping
Toxoplasmosis
Mouse bioassay
PCR-RFLP
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/124092
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-124092
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Genotyping
Toxoplasmosis
Mouse bioassay
PCR-RFLP
spellingShingle Ciencias Veterinarias
Genotyping
Toxoplasmosis
Mouse bioassay
PCR-RFLP
Camillo, Giovana
Machado, M. E. A.
Cadore, Gustavo Cauduro
Bräunig, Patricia
Venturini, María Cecilia
Pardini, Lais Luján
Barros, Luiz Daniel de
Garcia, João Luis
Sangioni, L. A.
Vogel, F. S. F.
<i>Toxoplasma gondii</i> genotyping from free-range chickens (<i>Gallus gallus domesticus</i>) in a rural area of Rio Grande do Sul, Brazil
topic_facet Ciencias Veterinarias
Genotyping
Toxoplasmosis
Mouse bioassay
PCR-RFLP
description Free-range chickens may ingest oocysts of T. gondii present in the environment and consequently harbor virulent strains of this parasite in different tissues, without any clinical signs. Isolation of T. gondii through bioassays on mice and cats from naturally infected chicken tissues has been described in several countries, demonstrating the importance of free-range chickens in the transmission of this parasite. The aim of this study was the genotypic characterization of T. gondii isolates obtained from naturally infected free-range chickens in a rural area of the state of Rio Grande do Sul, Brazil. Brain and heart tissue from 12 chickens seropositive for T. gondii were processed using peptic digestion technique for parasite isolation. From 12 samples subjected to mouse bioassay, nine isolates were obtained. RFLP-PCR genotypic characterization was performed using 11 genetic markers: SAG1, 5'-3'SAG2, alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1 and Apico. Genetic characterization of the isolates revealed the presence of five atypical genotypes according to ToxoDB (# 11, # 55, # 64, # 140 and # 163). Our results showed a wide genetic diversity of T. gondii in free-range chickens in this region.
format Articulo
Articulo
author Camillo, Giovana
Machado, M. E. A.
Cadore, Gustavo Cauduro
Bräunig, Patricia
Venturini, María Cecilia
Pardini, Lais Luján
Barros, Luiz Daniel de
Garcia, João Luis
Sangioni, L. A.
Vogel, F. S. F.
author_facet Camillo, Giovana
Machado, M. E. A.
Cadore, Gustavo Cauduro
Bräunig, Patricia
Venturini, María Cecilia
Pardini, Lais Luján
Barros, Luiz Daniel de
Garcia, João Luis
Sangioni, L. A.
Vogel, F. S. F.
author_sort Camillo, Giovana
title <i>Toxoplasma gondii</i> genotyping from free-range chickens (<i>Gallus gallus domesticus</i>) in a rural area of Rio Grande do Sul, Brazil
title_short <i>Toxoplasma gondii</i> genotyping from free-range chickens (<i>Gallus gallus domesticus</i>) in a rural area of Rio Grande do Sul, Brazil
title_full <i>Toxoplasma gondii</i> genotyping from free-range chickens (<i>Gallus gallus domesticus</i>) in a rural area of Rio Grande do Sul, Brazil
title_fullStr <i>Toxoplasma gondii</i> genotyping from free-range chickens (<i>Gallus gallus domesticus</i>) in a rural area of Rio Grande do Sul, Brazil
title_full_unstemmed <i>Toxoplasma gondii</i> genotyping from free-range chickens (<i>Gallus gallus domesticus</i>) in a rural area of Rio Grande do Sul, Brazil
title_sort <i>toxoplasma gondii</i> genotyping from free-range chickens (<i>gallus gallus domesticus</i>) in a rural area of rio grande do sul, brazil
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/124092
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