A simple, robust orthogonal background correction method for two-dimensional liquid chromatography

Background correction is a very important step that must be performed before peak detection or any quantification procedure. When successful, this step greatly simplifies such procedures and enhances the accuracy of quantification. In the past, much effort has been invested to correct drifting basel...

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Autores principales: Filgueira, Marcelo Roberto, Castells, Cecilia Beatriz Marta, Carr, Peter W.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2012
Materias:
Ciencias Exactas
Química
Diode array detector
Background correction
Chromatography
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/124057
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-124057
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Química
Diode array detector
Background correction
Chromatography
spellingShingle Ciencias Exactas
Química
Diode array detector
Background correction
Chromatography
Filgueira, Marcelo Roberto
Castells, Cecilia Beatriz Marta
Carr, Peter W.
A simple, robust orthogonal background correction method for two-dimensional liquid chromatography
topic_facet Ciencias Exactas
Química
Diode array detector
Background correction
Chromatography
description Background correction is a very important step that must be performed before peak detection or any quantification procedure. When successful, this step greatly simplifies such procedures and enhances the accuracy of quantification. In the past, much effort has been invested to correct drifting baseline in one-dimensional chromatography. In fast online comprehensive two-dimensional liquid chromatography (LC×LC) coupled with a diode array detector (DAD), the change in the refractive index (RI) of the mobile phase in very fast gradients causes extremely serious baseline disturbances. The method reported here is based on the use of various existing baseline correction methods of one-dimensional (1D) liquid chromatography to correct the two-dimensional (2D) background in LC×LC. When such methods are applied orthogonally to the second dimension (<sup>2</sup>D), background correction is dramatically improved. The method gives an almost zero mean background level and it provides better background correction than does simple subtraction of a blank. Indeed, the method proposed does not require running a blank sample.
format Articulo
Articulo
author Filgueira, Marcelo Roberto
Castells, Cecilia Beatriz Marta
Carr, Peter W.
author_facet Filgueira, Marcelo Roberto
Castells, Cecilia Beatriz Marta
Carr, Peter W.
author_sort Filgueira, Marcelo Roberto
title A simple, robust orthogonal background correction method for two-dimensional liquid chromatography
title_short A simple, robust orthogonal background correction method for two-dimensional liquid chromatography
title_full A simple, robust orthogonal background correction method for two-dimensional liquid chromatography
title_fullStr A simple, robust orthogonal background correction method for two-dimensional liquid chromatography
title_full_unstemmed A simple, robust orthogonal background correction method for two-dimensional liquid chromatography
title_sort simple, robust orthogonal background correction method for two-dimensional liquid chromatography
publishDate 2012
url http://sedici.unlp.edu.ar/handle/10915/124057
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AT carrpeterw asimplerobustorthogonalbackgroundcorrectionmethodfortwodimensionalliquidchromatography
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