Immunochemical reactivity of soybean β-conglycinin subunits

The immunochemical reactivity of two polyclonal sera raised against soybean purified αα' and β subunits was analysed. We show that anti-αα' serum mainly reacts with αα' subunits and β' subunit, but it does not recognize β subunit. In addition, β subunit is not able to compete wit...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Petruccelli, Silvana, Chirdo, Fernando Gabriel, Añón, María Cristina
Formato: Articulo
Lenguaje:Inglés
Publicado: 2005
Materias:
Química
β-conglycinin
Recombinant soybean subunits
Immunoreactivity
Soybean 7S globulin
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/123858
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-123858
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
β-conglycinin
Recombinant soybean subunits
Immunoreactivity
Soybean 7S globulin
spellingShingle Química
β-conglycinin
Recombinant soybean subunits
Immunoreactivity
Soybean 7S globulin
Petruccelli, Silvana
Chirdo, Fernando Gabriel
Añón, María Cristina
Immunochemical reactivity of soybean β-conglycinin subunits
topic_facet Química
β-conglycinin
Recombinant soybean subunits
Immunoreactivity
Soybean 7S globulin
description The immunochemical reactivity of two polyclonal sera raised against soybean purified αα' and β subunits was analysed. We show that anti-αα' serum mainly reacts with αα' subunits and β' subunit, but it does not recognize β subunit. In addition, β subunit is not able to compete with αα' subunit for anti-αα' serum recognition sites; therefore the main immunogenic region of αα' subunits is the extension region. Both anti-αα' and anti-β sera were able to recognize E. coli produced α subunit, consequently glycosylation is apparently not required for antigen-antibody interaction. Anti-β subunit serum shows strong reactivity against soybean produced β-subunit and recombinant α subunit, while it shows weak recognition of soybean purified αα' subunits. A mutant of α subunit with the N terminal β barrel region and C terminal α helices deleted, failed to be recognized by anti-β serum. For that reason although N and C terminal domains are structurally equivalent, their immunochemical reactivity is different.
format Articulo
Articulo
author Petruccelli, Silvana
Chirdo, Fernando Gabriel
Añón, María Cristina
author_facet Petruccelli, Silvana
Chirdo, Fernando Gabriel
Añón, María Cristina
author_sort Petruccelli, Silvana
title Immunochemical reactivity of soybean β-conglycinin subunits
title_short Immunochemical reactivity of soybean β-conglycinin subunits
title_full Immunochemical reactivity of soybean β-conglycinin subunits
title_fullStr Immunochemical reactivity of soybean β-conglycinin subunits
title_full_unstemmed Immunochemical reactivity of soybean β-conglycinin subunits
title_sort immunochemical reactivity of soybean β-conglycinin subunits
publishDate 2005
url http://sedici.unlp.edu.ar/handle/10915/123858
work_keys_str_mv AT petruccellisilvana immunochemicalreactivityofsoybeanbconglycininsubunits
AT chirdofernandogabriel immunochemicalreactivityofsoybeanbconglycininsubunits
AT anonmariacristina immunochemicalreactivityofsoybeanbconglycininsubunits
bdutipo_str Repositorios
_version_ 1764820450389524480

Ejemplares similares