First retrospective studies with etiological confirmation of porcine transmissible gastroenteritis virus infection in Argentina

Background: In 2014, a notification of porcine transmissible gastroenteritis virus (TGEV) was made by the National Services of Animal Health of Argentina (SENASA) to the World Organization of Animal Health (OIE). The notification was based on a serological diagnosis in a small farm with a morbidity...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Piñeyro, Pablo Enrique, Lozada, María Inés, Alarcón, Laura Valeria, Sanguinetti, Ramón, Cappuccio, Javier Alejandro, Pérez, Estefanía Marisol, Vannucci, Fabio A., Armocida, Alberto, Madson, Darin M., Perfumo, Carlos Juan, Quiroga, María Alejandra
Formato: Articulo
Lenguaje:Inglés
Publicado: 2018
Materias:
Ciencias Veterinarias
Diarrhea
Mortality
Piglets
Porcine transmissible gastroenteritis virus
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/123763
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-123763
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Diarrhea
Mortality
Piglets
Porcine transmissible gastroenteritis virus
spellingShingle Ciencias Veterinarias
Diarrhea
Mortality
Piglets
Porcine transmissible gastroenteritis virus
Piñeyro, Pablo Enrique
Lozada, María Inés
Alarcón, Laura Valeria
Sanguinetti, Ramón
Cappuccio, Javier Alejandro
Pérez, Estefanía Marisol
Vannucci, Fabio A.
Armocida, Alberto
Madson, Darin M.
Perfumo, Carlos Juan
Quiroga, María Alejandra
First retrospective studies with etiological confirmation of porcine transmissible gastroenteritis virus infection in Argentina
topic_facet Ciencias Veterinarias
Diarrhea
Mortality
Piglets
Porcine transmissible gastroenteritis virus
description Background: In 2014, a notification of porcine transmissible gastroenteritis virus (TGEV) was made by the National Services of Animal Health of Argentina (SENASA) to the World Organization of Animal Health (OIE). The notification was based on a serological diagnosis in a small farm with a morbidity rate of 2.3% without enteric clinical signs. In order to determine if TGEV was circulating before the official report, a retrospective study on cases of neonatal diarrhea was performed. The selection criteria was a sudden increase in mortality in 1- to 21-day-old piglets with watery diarrhea that did not respond to antibiotics. Based on these criteria, three clinical cases were identified during 2010–2015. Results: All animals that were evaluated presented histological lesions consistent with enteric viral infection. The feces and ultrathin sections of intestine that were evaluated by electron microscopy confirmed the presence of round particles of approximately 80 nm in size and characterized by finely granular electrodense nucleoids consistent with complete particles of coronavirus. The presence of the TGEV antigen was confirmed by monoclonal specific immunohistochemistry, and final confirmation of a metabolically-active virus was performed by in situ hybridization to detect a TGE mRNA encoding spike protein. All sections evaluated in this case were negative for PEDV and rotavirus A. Conclusions: This is the first case series describing neonatal mortality with etiological confirmation of TGEV in Argentina. The clinical diagnosis of TGEV infections in endemic regions is challenging due to the epidemiological distribution and coinfection with other enteric pathogens that mask the clinical presentation.
format Articulo
Articulo
author Piñeyro, Pablo Enrique
Lozada, María Inés
Alarcón, Laura Valeria
Sanguinetti, Ramón
Cappuccio, Javier Alejandro
Pérez, Estefanía Marisol
Vannucci, Fabio A.
Armocida, Alberto
Madson, Darin M.
Perfumo, Carlos Juan
Quiroga, María Alejandra
author_facet Piñeyro, Pablo Enrique
Lozada, María Inés
Alarcón, Laura Valeria
Sanguinetti, Ramón
Cappuccio, Javier Alejandro
Pérez, Estefanía Marisol
Vannucci, Fabio A.
Armocida, Alberto
Madson, Darin M.
Perfumo, Carlos Juan
Quiroga, María Alejandra
author_sort Piñeyro, Pablo Enrique
title First retrospective studies with etiological confirmation of porcine transmissible gastroenteritis virus infection in Argentina
title_short First retrospective studies with etiological confirmation of porcine transmissible gastroenteritis virus infection in Argentina
title_full First retrospective studies with etiological confirmation of porcine transmissible gastroenteritis virus infection in Argentina
title_fullStr First retrospective studies with etiological confirmation of porcine transmissible gastroenteritis virus infection in Argentina
title_full_unstemmed First retrospective studies with etiological confirmation of porcine transmissible gastroenteritis virus infection in Argentina
title_sort first retrospective studies with etiological confirmation of porcine transmissible gastroenteritis virus infection in argentina
publishDate 2018
url http://sedici.unlp.edu.ar/handle/10915/123763
work_keys_str_mv AT pineyropabloenrique firstretrospectivestudieswithetiologicalconfirmationofporcinetransmissiblegastroenteritisvirusinfectioninargentina
AT lozadamariaines firstretrospectivestudieswithetiologicalconfirmationofporcinetransmissiblegastroenteritisvirusinfectioninargentina
AT alarconlauravaleria firstretrospectivestudieswithetiologicalconfirmationofporcinetransmissiblegastroenteritisvirusinfectioninargentina
AT sanguinettiramon firstretrospectivestudieswithetiologicalconfirmationofporcinetransmissiblegastroenteritisvirusinfectioninargentina
AT cappucciojavieralejandro firstretrospectivestudieswithetiologicalconfirmationofporcinetransmissiblegastroenteritisvirusinfectioninargentina
AT perezestefaniamarisol firstretrospectivestudieswithetiologicalconfirmationofporcinetransmissiblegastroenteritisvirusinfectioninargentina
AT vannuccifabioa firstretrospectivestudieswithetiologicalconfirmationofporcinetransmissiblegastroenteritisvirusinfectioninargentina
AT armocidaalberto firstretrospectivestudieswithetiologicalconfirmationofporcinetransmissiblegastroenteritisvirusinfectioninargentina
AT madsondarinm firstretrospectivestudieswithetiologicalconfirmationofporcinetransmissiblegastroenteritisvirusinfectioninargentina
AT perfumocarlosjuan firstretrospectivestudieswithetiologicalconfirmationofporcinetransmissiblegastroenteritisvirusinfectioninargentina
AT quirogamariaalejandra firstretrospectivestudieswithetiologicalconfirmationofporcinetransmissiblegastroenteritisvirusinfectioninargentina
bdutipo_str Repositorios
_version_ 1764820450231189504

Ejemplares similares