Identification and molecular epidemiology of methicillin resistant Staphylococcus pseudintermedius strains isolated from canine clinical samples in Argentina

Staphylococcus pseudintermedius is the leading cause of pyoderma in dogs and the frequent use of antimicrobial treatment is associated to the development of resistance to nearly all classes of antibiotics. Despite S. pseudintermedius significance, our understanding of the molecular mechanism of β-la...

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Autores principales: Gagetti, Paula, Wattam, Alice R., Giacoboni, Gabriela Isabel, De Paulis, Adriana N., Bertona, Eugenia, Corso, Alejandra, Rosato, Adriana E.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2019
Materias:
Zoonosis
Antibiotic resistance
MRSP
Small animals
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/123677
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-123677
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Zoonosis
Antibiotic resistance
MRSP
Small animals
spellingShingle Zoonosis
Antibiotic resistance
MRSP
Small animals
Gagetti, Paula
Wattam, Alice R.
Giacoboni, Gabriela Isabel
De Paulis, Adriana N.
Bertona, Eugenia
Corso, Alejandra
Rosato, Adriana E.
Identification and molecular epidemiology of methicillin resistant Staphylococcus pseudintermedius strains isolated from canine clinical samples in Argentina
topic_facet Zoonosis
Antibiotic resistance
MRSP
Small animals
description Staphylococcus pseudintermedius is the leading cause of pyoderma in dogs and the frequent use of antimicrobial treatment is associated to the development of resistance to nearly all classes of antibiotics. Despite S. pseudintermedius significance, our understanding of the molecular mechanism of β-lactam resistance and its genetic diversity remains limited. We aimed to: i) determine the phenotypic resistance profile of methicillin resistant Staphylococcus pseudintermedius (MRSP) isolated from infected dogs in three different veterinary hospitals in Buenos Aires, Argentina; ii) identify the SCCmec elements and resistance genes; and iii) analyze the clonal relationship between isolates and in regard of dominant lineages found in the world. In addition to the differential levels of β-lactam resistance, MRSP isolates (n = 10) showed resistance to 5–6 families of antibiotics, and were therefore categorized as multidrug-resistant. All the isolates were variant of SCCmec V homologous to S. aureus; additional SCCmecFinder analysis classified five of the genomes as SCCmec type V (5C2&5) with mecA (encodes for PBP2a), mecRI and mecI and all the genes closely related to the reference SCCmec type V S. aureus TSGH17 strain. In the remaining five strains, mecA was present, although other genes associated with SCCmec V including mecR1 and mecI were missing. PBP2a was inducible in low level resistance strains (MRSP 8151), and constitutively expressed in MRSP 8150, suggesting different mecA regulatory mechanisms. MRSP isolates showed significant genetic diversity: eight PFGE clonal types and six multilocus-sequence typing (MLST) sequence types (STs) (339, 649, 919, 920, 921 and 922), including four new STs genetically distinct from STs reported in other geographic areas. Comparative genomics and phylogenetic analyses of the MRSP showed a correlation between the genetic content and the phenotypes, and established the genetic relationship between the isolates. MRSP could be a threat to animal health due to it concerning level of antimicrobial resistance. Our study highlights genetic and epidemiological aspects of multidrug-resistant MRSP strains from Argentina showing high degree of correlation between the resistance genes and the phenotype of the isolates and, furthermore, they appeared evolutionary closer to major worldwide reported ST68 and ST71.
format Articulo
Articulo
author Gagetti, Paula
Wattam, Alice R.
Giacoboni, Gabriela Isabel
De Paulis, Adriana N.
Bertona, Eugenia
Corso, Alejandra
Rosato, Adriana E.
author_facet Gagetti, Paula
Wattam, Alice R.
Giacoboni, Gabriela Isabel
De Paulis, Adriana N.
Bertona, Eugenia
Corso, Alejandra
Rosato, Adriana E.
author_sort Gagetti, Paula
title Identification and molecular epidemiology of methicillin resistant Staphylococcus pseudintermedius strains isolated from canine clinical samples in Argentina
title_short Identification and molecular epidemiology of methicillin resistant Staphylococcus pseudintermedius strains isolated from canine clinical samples in Argentina
title_full Identification and molecular epidemiology of methicillin resistant Staphylococcus pseudintermedius strains isolated from canine clinical samples in Argentina
title_fullStr Identification and molecular epidemiology of methicillin resistant Staphylococcus pseudintermedius strains isolated from canine clinical samples in Argentina
title_full_unstemmed Identification and molecular epidemiology of methicillin resistant Staphylococcus pseudintermedius strains isolated from canine clinical samples in Argentina
title_sort identification and molecular epidemiology of methicillin resistant staphylococcus pseudintermedius strains isolated from canine clinical samples in argentina
publishDate 2019
url http://sedici.unlp.edu.ar/handle/10915/123677
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